Non-thermal dielectric barrier discharge (DBD) plasma is an innovative and emerging field combining plasma physics, life science and clinical medicine for a wide-range of biological applications. Plasma techniques are applied in treating surfaces, materials or devices to realize specific qualities for subsequent special medical applications, plant seeds to improve the production and quality of crops, and living cells or tissues to realize therapeutic effects. Several studies that are summarized within this review show that non-thermal DBD plasma technique has potential biological applications in soybean sprout growth, chicken embryonic development and postnatal growth rate, and even male chicken reproductive capacity. The current developments in the non-thermal DBD plasma technique may be beneficial to improve plant and poultry productivity.