Low temperature tolerance, cold hardening and acclimation in tadpoles of the neotropical túngara frog ( Engystomops pustulosus )

Vo, Pacific; Gridi‐Papp, Marcos

doi:10.1016/j.jtherbio.2017.03.012

Cited by 10 publications

(14 citation statements)

References 59 publications

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“…Further work is required to understand the potential for environ- Knowles & Weigl, 1990;Putnam & Bennett, 1981;Renaud & Stevens, 1983;Rome, 1983;Whitehead, Puckridge, Leigh, & Seymour, 1989;Wilson & Franklin, 2000), aquatic life stages are more capable (Vo & Gridi-Papp, 2017;Wilson & Franklin, 1999).…”

Section: F I G U R Ementioning

confidence: 99%

Cooler temperatures slow the repair of DNA damage in tadpoles exposed to ultraviolet radiation: Implications for amphibian declines at high altitude

Morison

Cramp

Alton

et al. 2019

Global Change Biology

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Ultraviolet B radiation (UVBR) damages the DNA of exposed cells, causing dimers to form between adjacent pyrimidine nucleotides. These dimers block DNA replication, causing mutations and apoptosis. Most organisms utilize biochemical or biophysical DNA repair strategies to restore DNA structure; however, as with most biological reactions, these processes are likely to be thermally sensitive. Tadpoles exposed to elevated UVBR at low environmental temperatures have significantly higher rates of mortality and developmental deformities compared with tadpoles exposed to the same levels of UVBR at higher environmental temperatures. We hypothesized that low environmental temperatures impair the primary enzymatic (photolyase) DNA repair pathway in amphibians, leading to the accumulation of DNA damage. To test this hypothesis, we compared DNA repair rates and photolyase gene expression patterns in Limnodynastes peronii. Tadpoles were acutely exposed to UVBR for 1 hr at either 20 or 30°C, and we measured DNA damage and photolyase expression levels at intervals following this exposure. Temperature had a significant effect on the rate of DNA repair, with repair at 30°C occurring twice as fast as repair at 20°C. Photolyase gene expression (6‐4 PP and CPD) was significantly upregulated by UVBR exposure, with expression levels increasing within 6 hr of UVBR exposure. CPD expression levels were not significantly affected by temperature, but 6‐4 PP expression was significantly higher in tadpoles in the 30°C treatment within 12 hr of UVBR exposure. These data support the hypothesis that DNA repair rates are thermally sensitive in tadpoles and may explain why enigmatic amphibian declines are higher in montane regions where UVBR levels are naturally elevated and environmental temperatures are lower.

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Section: F I G U R Ementioning

confidence: 99%

Cooler temperatures slow the repair of DNA damage in tadpoles exposed to ultraviolet radiation: Implications for amphibian declines at high altitude

Morison

Cramp

Alton

et al. 2019

Global Change Biology

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“…The acclimation ability of túngara frogs protects them from climatic changes providing a tolerance flexibility range of 1.1°C toward high and 1.7°C toward low temperatures. They can also respond within an hour to a brief cold exposure with rapid cold hardening, lowering their CTMin by 1.18°C (Vo & Gridi‐Papp ). This plasticity in CTMax and CTMin is important for the prediction of the adaptive capacity of túngara frog because it expands the range of temperatures to which the animal can be exposed without harm.…”

Section: Discussionmentioning

confidence: 99%

“…Their CTMin shows that they can tolerate cold temperatures 11.5°C (cooling tolerance) lower than the minimum environmental temperature. Another indication of adaptation capacity to cold in this species is its cold hardening ability (Vo & Gridi‐Papp ). Although CTMin data for other species in the same latitude are not available for comparison, a study on 27 tropical lowland species from South America (13°54′S to 15°24′S, Gutiérrez‐Pesquera et al .…”

Section: Discussionmentioning

confidence: 99%

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Thermal sensitivity of a Neotropical amphibian (Engystomops pustulosus) and its vulnerability to climate change

Oyamaguchi

Grewal

et al. 2017

Biotropica

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A species’ thermal sensitivity and its exposure to climate variation are key components in the prediction of its vulnerability to climate change. We tested the thermal sensitivity of a tropical amphibian that lives in a mild constant climate in which the thermal tolerance range is expected to closely match the experienced environmental temperature. The air temperature that this species is exposed to varies between 21.9 and 31.6°C with an annual mean of 27.2°C. We estimated the microhabitat water temperature variation under vegetation shade, which buffers the temperature by 1.8°C in relation to that of the air, and with open canopy, where the water was 1.9°C warmer than the air temperature. With broods of tadpoles split into five treatments (15°C, 21°C, 28°C, 31°C, and 33°C), we estimated the critical thermal maximum (CTMax) and critical thermal minimum (CTMin) after at least 7 days of acclimation. Both CTMax (42.3°C) and CTMin (11.8°C) were more extreme than the temperature range estimated for the field. We estimated the optimum temperature (To = 28.8°C) and the thermal performance breadth (range: 23.3–34.1°C) based on growth rate (g/day). The animals were able to acclimate more extensively to cold than to warm temperatures. These performance curve traits closely matched the air temperature. The estimated vulnerability varied according to the microhabitat prediction model used. The combination of tadpole data on thermal sensitivity and macro‐ and microhabitat variation provides a necessary framework to understand the effects of climate change on tropical amphibians.

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“…For example, in the cane toad Rhinella marina acclimation to 12°C for 12 h reduces the critical thermal minimum (CT min ) (see Glossary) by ∼2°C, compared with those maintained at 24°C (McCann et al, 2014). Also, in tadpoles of the neotropical túngara frog Engystomops pustulosus a prior induction of chill coma (see Glossary) by cooling at 1.0°C min −1 slightly depresses CT min during a subsequent trial (Vo and Gridi-Papp, 2017). Similarly, some species of fish (Hazel and Landrey, 1988), salamanders (Layne and Claussen, 1987) and turtles (Muir et al, 2010) exhibit RHC-like responses.…”

Section: Ecological Relevance Of Rch the Rch Response Is Widespread Amentioning

confidence: 99%

Rapid cold hardening: ecological relevance, physiological mechanisms and new perspectives

Teets

Gantz

Kawarasaki

2020

Journal of Experimental Biology

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Rapid cold hardening (RCH) is a type of phenotypic plasticity that allows ectotherms to quickly enhance cold tolerance in response to brief chilling (lasting minutes to hours). In this Review, we summarize the current state of knowledge of this important phenotype and provide new directions for research. As one of the fastest adaptive responses to temperature known, RCH allows ectotherms to cope with sudden cold snaps and to optimize their performance during diurnal cooling cycles. RCH and similar phenotypes have been observed across a diversity of ectotherms, including crustaceans, terrestrial arthropods, amphibians, reptiles, and fish. In addition to its well-defined role in enhancing survival to extreme cold, RCH also protects against nonlethal cold injury by preserving essential functions following cold stress, such as locomotion, reproduction, and energy balance. The capacity for RCH varies across species and across genotypes of the same species, indicating that RCH can be shaped by selection and is likely favored in thermally variable environments. Mechanistically, RCH is distinct from other rapid stress responses in that it typically does not involve synthesis of new gene products; rather, the existing cellular machinery regulates RCH through post-translational signaling mechanisms. However, the protective mechanisms that enhance cold hardiness are largely unknown. We provide evidence that RCH can be induced by multiple triggers in addition to low temperature, and that rapidly induced tolerance and cross-tolerance to a variety of environmental stressors may be a general feature of stress responses that requires further investigation.

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Low temperature tolerance, cold hardening and acclimation in tadpoles of the neotropical túngara frog ( Engystomops pustulosus )

Cited by 10 publications

References 59 publications

Cooler temperatures slow the repair of DNA damage in tadpoles exposed to ultraviolet radiation: Implications for amphibian declines at high altitude

Cooler temperatures slow the repair of DNA damage in tadpoles exposed to ultraviolet radiation: Implications for amphibian declines at high altitude

Thermal sensitivity of a Neotropical amphibian (Engystomops pustulosus) and its vulnerability to climate change

Rapid cold hardening: ecological relevance, physiological mechanisms and new perspectives

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