Low Temperature Treatment Affects Concentration and Distribution of Chrysanthemum Stunt Viroid in Argyranthemum

Zhang, Zhibo; Lee, YeonKyeong; Sivertsen, Astrid; Skjeseth, Gry; Haugslien, Sissel; Clarke, Jihong Liu; Wang, Qiao‐Chun; Blystad, Dag‐Ragnar

doi:10.3389/fmicb.2016.00224

Cited by 2 publications

(1 citation statement)

References 43 publications

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“…Both high temperature, e.g., 37-40 • C, for control of Grapevine leafroll-associated virus 1 (GLRaV-1) and Grapevine rupestris stem pitting-associated virus (GRSPaV), a difficult to eradicate virus, from grapevine "Agiorgitiko" [17], as well as low temperature, approx. 5 • C, in chrysanthemum production free from Chrysanthemum stunt viroid (CSVd) are applied [18].…”

Section: Selected Physical Methods Of Plant Protection Useful In Storagementioning

confidence: 99%

Physical Crop Postharvest Storage and Protection Methods

et al. 2021

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Sustainable and organic plant production uses natural products and natural self-regulation processes occurring in the ecosystem. The awareness is growing and the demands of consumers are higher and higher. One solution is to use various methods, as an alternative to pesticides. It is also very important to care for the stored crops after harvesting especially using non-chemical methods. The physical method of plant protection consists in treating the harmful organism with physical factors such as temperature, its same light and radiation, controlled atmosphere, special packaging, pressure, various sounds, ozone, and low-temperature plasma. The availability of effective application techniques opens up new possibilities for the storage of crops in order to maintain their health and quality for a long time. This review focuses on the analysis of physical methods of postharvest protection, especially the latest methods using ozone and low-temperature plasma. As a result, consumers of agricultural crops will be able to consume food free of insects, mycotoxins and pesticide residues.

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Section: Selected Physical Methods Of Plant Protection Useful In Storagementioning

confidence: 99%

Physical Crop Postharvest Storage and Protection Methods

et al. 2021

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The Elimination of Viroids through In Vitro Thermotherapy and a Meristem Tip Culture from a New Limonime Hybrid (Citrus x limon var. limon (L.) Burm. f. x Citrus latifolia var. latifolia)

Sarropoulou,

Grigoriadou,

Maliogka

et al. 2024

BioTech

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Viruses and viroids pose a significant challenge in citriculture, and their control is crucial for plant health. This study evaluated the effectiveness of in vitro thermotherapy combined with a meristem tip culture for eliminating citrus exocortis viroid (CEVd) and hop stunt viroid (HSVd) from a new limonime hybrid (Citrus x limon var. limon x Citrus latifolia var. latifolia). The elimination success was confirmed by RT-PCR assays. The in vitro elimination rate for CEVd during the shoot proliferation stage (43%) was higher than for HSVd (21%). Accordingly, in the subsequent rooting stage, the in vitro elimination rate for CEVd (50%) was higher than for HSVd (33%). Successful CEVd and HSVd eradication at a 100% rate was confirmed in the ex vitro acclimatized plants in the greenhouse. The study also established an efficient micropropagation protocol. The optimal treatment for in vitro shoot induction was 0.5–2 mg L−1 benzyladenine (BA) + 0.5 mg L−1 gibberellic acid (GA3) + 0.25 mg L−1 naphthalene acetic acid (NAA), while for shoot elongation, it was 0.5 mg L−1 BA + 0.5 mg L−1 kinetin (KIN) + 0.5 mg L−1 GA3 + 0.25 mg L−1 NAA. Rooting was best promoted by 1 mg L−1 NAA. This study provides valuable insights for the mass production of viroid-free propagation material in this new lemon x lime hybrid, contributing to the conservation of genetic resources in citrus breeding programs through the combined application of in vitro thermotherapy and an in vitro meristem tip culture, a novel and highlighted achievement reported for the first time in this study.

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Low Temperature Treatment Affects Concentration and Distribution of Chrysanthemum Stunt Viroid in Argyranthemum

Cited by 2 publications

References 43 publications

Physical Crop Postharvest Storage and Protection Methods

Physical Crop Postharvest Storage and Protection Methods

The Elimination of Viroids through In Vitro Thermotherapy and a Meristem Tip Culture from a New Limonime Hybrid (Citrus x limon var. limon (L.) Burm. f. x Citrus latifolia var. latifolia)

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