Low testosterone levels induce apoptosis via active 3-caspase dependent signaling in the bladder wall of male rats

Lorenzetti, Fábio; Pintarelli, Vitor; Seraphim, Deborah; Dambros, Míriam

doi:10.3109/13685538.2012.716876

Cited by 8 publications

(9 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The increased apoptotic index induced by androgen ablation may explain the decrease in smooth muscle content observed after castration [1] and the decrease in penile size after penile denervation [13] . Although there are strong evidences showing the effects of T withdrawal and replacement on apoptosis regulation [1] , [8] , our results indicate that apoptosis was not influenced by long-term androgen deprivation and replacement, which are in agreement with some studies published so far [17] , [19] , [20] . Nakazawa et al.…”

Section: Discussionsupporting

confidence: 93%

Testosterone replacement maintains smooth muscle content in the corpus cavernosum of orchiectomized rats

Halmenschlager

Rhoden

Motta

et al. 2017

Asian Journal of Urology

View full text Add to dashboard Cite

ObjectiveTo evaluate the effects of testosterone (T) on the maintenance of corpus cavernosum (CC) structure and apoptosis.MethodsAnimals were divided into three groups: sham operation group (n = 8) underwent sham operation; Orchiectomized (Orchiec)+ oily vehicle group (n = 8) underwent bilateral orchiectomy and received a single dose of oily vehicle by intramuscular injection (i.m.) 30 days after orchiectomy; and Orchiec + T group (n = 8) underwent bilateral orchiectomy and received a single dose of T undecanoate 100 mg/kg i.m. 30 days after the surgery. Animals were euthanized 60 days after the beginning of the experiment with an anesthetic overdose of ketamine and xylazine. Blood samples and penile tissue were collected on euthanasia. Azan's trichrome staining was used to evaluate smooth muscle, Weigert's Fucsin-Resorcin staining was used to evaluate elastic fibers and Picrosirius red staining was used to evaluate collagen. Apoptosis was evaluated using TUNEL technique.ResultsT levels decreased in Orchiec + oily vehicle when compared to sham operation and Orchiec + T groups (p < 0.001). T deprivation reduced trabecular smooth muscle content and penile diameter and T replacement maintained both parameters (p = 0.005 and p = 0.001, respectively). No difference was observed in the content of sinusoidal space (p = 0.207), elastic fibers (p = 0.849), collagen (p = 0.216) and in apoptosis (p = 0.095).ConclusionNormal testosterone levels maintain CC smooth muscle content and do not influence elastic fibers, collagen content and apoptotic index. Further studies should be performed in order to investigate the mechanisms by which androgen mediates its effects on CC structure.

show abstract

Section: Discussionsupporting

confidence: 93%

Testosterone replacement maintains smooth muscle content in the corpus cavernosum of orchiectomized rats

Halmenschlager

Rhoden

Motta

et al. 2017

Asian Journal of Urology

View full text Add to dashboard Cite

show abstract

“…The pathophysiological mechanisms of LUTS encompass prostate enlargement, alterations in urethra histological structure bladder fibrosis and alterations in pelvic neuronal and vascular networks, thus contributing to lower urinary tract dysfunction [154155]. Androgen deficiency in the animal model is reported to produce marked alterations in the bladder wall structure as shown by decreased elastic fibers and increased collagen fiber density, a pathology that is reversible by T treatment [159160]. This observation has clinical implication as reported by Yassin et al [145161] in which T therapy was shown to reduce bladder wall thickness interruption of T therapy led to increase in bladder wall thickness.…”

Section: Discussionmentioning

confidence: 99%

Impact of Testosterone Deficiency and Testosterone Therapy on Lower Urinary Tract Symptoms in Men with Metabolic Syndrome

Traish

Johansen

2018

World J Mens Health

View full text Add to dashboard Cite

Lower urinary tract function is modulated by neural, vascular and urethral and bladder structural elements. The pathophysiological mechanisms of lower urinary tract symptoms (LUTS) encompass prostate enlargement, alterations in urethra histological structure bladder fibrosis and alterations in pelvic neuronal and vascular networks, The complex pathophysiological relationship between testosterone (T) deficiency (TD) and the constellations LUTS, and metabolic dysfunction manifested in the metabolic syndrome (Met S) remains poorly understood. TD has emerged as one the potential targets by which Met S may contribute to the onset and development as well as worsening of LUTS. Because it has been recognized that treatment of men with Met S with T therapy ameliorates Met S components, it is postulated that T therapy may represent a therapeutic target in improving LUTS. Furthermore, the effect of TD on the prostate remains unclear, and often debatable. It is believed that T exclusively promotes prostate growth, however recent evidence has strongly contradicted this belief. The true relationship between benign prostatic hyperplasia, TD, and LUTS remains elusive and further research will be required to clarify the role of T in both benign prostatic hypertrophy (BPH) and LUTS as a whole. Although there is conflicting evidence about the benefits of T therapy in men with BPH and LUTS, the current body of literature supports the safety of using this therapy in men with enlarged prostate. As the population afflicted with obesity epidemic continues to age, the number of men suffering from Met S and LUTS together is expected to increase.

show abstract

“…Further research has confirmed that testosterone supplementation exerts both beneficial and pathological effects on apoptotic process. Depletion of androgen below normal plasma levels has been found to increase kainate-induced neuronal loss ( 61 ) and induce apoptosis in the bladder wall of senile male rats ( 62 ), while elevated testosterone has been observed to induce apoptosis in a human neuroblastoma cell line ( 63 ). These findings indicate that the beneficial effects of androgen are obtained when androgen levels are within a specific (nanomolar) physiological range, the regulation of which is crucial for normal muscular functioning and a deviation from which in either direction could be deleterious to muscle health.…”

Section: Cellular and Molecular Mechanisms Underlying Sarcopeniamentioning

confidence: 99%

Sarcopenia and Androgens: A Link between Pathology and Treatment

et al. 2014

View full text Add to dashboard Cite

Sarcopenia, the age-related loss of skeletal muscle mass and function, is becoming more prevalent as the lifespan continues to increase in most populations. As sarcopenia is highly disabling, being associated with increased risk of dependence, falls, fractures, weakness, disability, and death, development of approaches to its prevention and treatment are required. Androgens are the main physiologic anabolic steroid hormones and normal testosterone levels are necessary for a range of developmental and biological processes, including maintenance of muscle mass. Testosterone concentrations decline as age increase, suggesting that low plasma testosterone levels can cause or accelerate muscle- and age-related diseases, as sarcopenia. Currently, there is increasing interest on the anabolic properties of testosterone for therapeutic use in muscle diseases including sarcopenia. However, the pathophysiological mechanisms underlying this muscle syndrome and its relationship with plasma level of androgens are not completely understood. This review discusses the recent findings regarding sarcopenia, the intrinsic, and extrinsic mechanisms involved in the onset and progression of this disease and the treatment approaches that have been developed based on testosterone deficiency and their implications.

show abstract

Low testosterone levels induce apoptosis via active 3-caspase dependent signaling in the bladder wall of male rats

Abstract: We can state that low plasma testosterone levels are related to higher collagen fiber density and active 3-caspase percentages in the bladder walls of orchiectomized and senile rats, respectively.

Cited by 8 publications

References 21 publications

Testosterone replacement maintains smooth muscle content in the corpus cavernosum of orchiectomized rats

Testosterone replacement maintains smooth muscle content in the corpus cavernosum of orchiectomized rats

Impact of Testosterone Deficiency and Testosterone Therapy on Lower Urinary Tract Symptoms in Men with Metabolic Syndrome

Sarcopenia and Androgens: A Link between Pathology and Treatment

Contact Info

Product

Resources

About