Low vitamin D-modulated calcium-regulating proteins in psoriasis vulgaris plaques: S100A7 overexpression depends on joint involvement

Abstract

Psoriasis is an inflammatory skin disease with or without joint involvement. In this disease, the thickened epidermis and impaired barrier are associated with altered calcium gradients. Calcium and vitamin D are known to play important roles in keratinocyte differentiation and bone metabolism. Intracellular calcium is regulated by calcium-sensing receptor (CASR), calcium release-activated calcium modulator (ORAI) and stromal interaction molecule (STIM). Other proteins modulated by vitamin D play important roles in calcium regulation e.g., calbindin 1 (CALB1) and transient receptor potential cation channel 6 (TRPV6). In this study, we aimed to investigate the expression of calcium-regulating proteins in the plaques of patients with psoriasis vulgaris with or without joint inflammation. We confirmed low calcium levels, keratinocyte hyperproliferation and an altered epidermal barrier. The CASR, ORAI1, ORAI3, STIM1, CALB1 and TRPV6 mRNA, as well as the sterol 27-hydroxylase (CYP27A1), 25-hydroxyvitamin D3 1-α-hydroxylase (CYP27B1) and 1,25-dihydroxyvitamin D3 24-hydroxylase (CYP24A1) protein levels were low in the plaques of patients with psoriasis. We demonstrated S100 calcium-binding protein A7 (S100A7) overexpression in the plaques of patients with psoriasis vulgaris with joint inflammation, compared with those without joint involvement. We suggest an altered capacity to regulate the intracellular Ca2+ concentration ([Ca2+]i), characterized by a reduced expression of CASR, ORAI1, ORAI3, STIM1, CALB1 and TRPV6 associated with diminished levels of 1,25-dihydroxyvitamin D3 [1,25(OH)2D3], which may be associated with an altered balance between keratinocyte proliferation and differentiation in the psoriatic epidermis. Additionally, differences in S100A7 expression depend on the presence of joint involvement.

The deregulation of STIM1 and store operative calcium entry impaired aortic smooth muscle cells contractility in aortic medial degeneration

“…Previous reports have indicated that STIM1 has no apparent role in normal ASMCs [7], but recent microarray analysis of clinical samples has provided intriguing evidence that STIM1 may be a factor in AMD [8]. Evidence suggests that vitamin D deficiency suppresses the expression of a number of calcium-regulating proteins, including STIM1 [9,10]. As is well known, AMD is closely related to the process of ageing.…”

Profiling of Sika Deer Antler Proteins at Different Developmental Stages Based on Label-free

“…These immune-related molecules play an important role in the regulation of osteoblast and osteoclast differentiation. S100A7 promotes osteoclast differentiation and activity by enhancing the effects of M-CSF and RANKL [34][35][36] . However, several other factors effectively promote the proliferation and differentiation of osteoblasts and bone formation while inhibiting bone resorption [37][38][39][40][41][42][43][44] .…”

Genetic regulators of mineral amount in Nelore cattle muscle predicted by a new co-expression and regulatory impact factor approach

“…A homolog of psoriasin, a common protein in psoriasis patients, was identified in bovines and had the same antimicrobial and immune response activity as the human protein 44 . Further, Ca and vitamin D play important roles in keratinocyte differentiation and regulate proteins involved in psoriasis 45 and S is used as a known treatment and prevention of recurrence for this disease 46 . Our results suggest the genes whose expressions were correlated to bta-miR-369-3p expression as new candidate targets of this miRNA, linked to immune response and mineral concentration.…”