Low vitamin E in diet reduces stress resistance of gilthead seabream (Sparus aurata) juveniles

Montero, Daniel; Tort, L.; Robaina, L.; Vergara, J.M.; Izquierdo, M.S.

doi:10.1006/fsim.2000.0324

Cited by 130 publications

(107 citation statements)

References 50 publications

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“…The increase recorded 4 hours after intraperitoneal injection is in contrast with previous research showing the progressive decrease in haemagglutination activity in gilthead seabream juveniles exposed to repeated acute stress consisting in chasing with a net for 5 minutes over 14 days [7,71]. In the same species, chronic stress induced by crowding caused immunodepression, as suggested by decreases in haemagglutination titre observed after 9 days [72].…”

Section: Discussioncontrasting

confidence: 81%

Haematological and Immunological Responses in Juvenile Sea Bass (Dicentrarchus labrax L.) After Short-Term Acute Stress

Maricchiolo¹,

Caruso²,

Genovese³

2008

TOFISHSJ

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Abstract:The physiological effects of acute stress (induced by an intraperitoneal injection) on the haematocrit, haemoglobin, serum cortisol and glucose, haemagglutinins and lysozyme were investigated in juvenile sea bass Dicentrarchus labrax. A total of 40 fish were subjected to an intraperitoneal injection. Eight fish were sampled at 0, 2, 4, 24 and 48 h after acute stress and compared to unstressed fish (controls) sacrificed at the same times. Intraperitoneal injection resulted in significant changes in cortisol and glucose 4 and 2 hours after exposure to stress, respectively, but not in haematocrit and haemoglobin levels. No significant differences between stressed and control fish were recorded for lysozyme content and haemagglutinating activity throughout the experiment. In juvenile sea bass acute stress caused by intraperitoneal injection was mostly associated with the increase in common stress indicators (cortisol and glucose), while no clear response in terms of non-specific immune parameters was evidenced.

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Section: Discussioncontrasting

confidence: 81%

Haematological and Immunological Responses in Juvenile Sea Bass (Dicentrarchus labrax L.) After Short-Term Acute Stress

Maricchiolo¹,

Caruso²,

Genovese³

2008

TOFISHSJ

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“…In the present study, HSD induced chronic stress that was responsible for elevation of plasma cortisol, blood glucose and erythrocytes. This corroborated the observation by Abdel-Tawwab (2012) and Montero et al (2001), in which elevated stocking density-related stress, raised levels of cortisol, glucose and erythrocytes in Nile tilapia and Gilthead Seabream Sparus aurata. However, the stocking density used in this study was higher than that used by Abdel-Tawwab (2012) and as such it elicited higher values of plasma cortisol, blood glucose and erythrocytes in this study as compared to the corresponding values in the former study.…”

Section: Effect Of Stocking Density On Plasma Cortisol Level Blood Gsupporting

confidence: 90%

“…However, the erythrocytes counts obtained from HSD and LSD groups fell within erythrocyte count range provided by Bittencourt et al (2003) for Nile tilapia. The rise in red blood cell numbers in fish held at HSD is similar to patterns described in previous studies (Montero et al 2001;Bittencourt et al 2003;Dobšikova et al 2009;Abdel-Tawwab 2012) in which stress enhanced the numbers of erythrocytes. Falcon et al (2007) observed that stress lead to elevation of the levels of cortisol that in turn lead to increased erythropoietin concentration stimulating erythrocytes production.…”

Section: Effect Of Stocking Density On Plasma Cortisol Level Blood Gsupporting

confidence: 88%

“…High stocking density (HSD) is one such method that has been used to increase fish productivity (Chakraborty et al 2010;Salama et al 2006). However, HSD causes chronic stress to fish (Montero et al 2001;El-Khaldi 2010) leading to decreased growth, fin erosion, disruption of reproductive capacities, and increase in disease susceptibility and mortality (Pickering 1993;Schreck 1996).…”

Section: Introductionmentioning

confidence: 99%

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Effect of stocking density on the expression of glucose transporter protein 1 and other physiological factors in the Lake Victoria Nile tilapia, Oreochromis niloticus (L.)

et al. 2014

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Fish farmers have a tendency of employing high stocking density (HSD) as a means of increasing productivity. However, HSD is a chronic stressor that is likely to lower profitability of fish farming if not implemented properly. HSD induces stress which in turn elevates sequentially the levels of plasma cortisol and glucose. The resultant glucose is distributed to various tissues by glucose transporter protein (GLUTs) to restore normalcy. GLUT 1, transmembrane protein found in erythrocytes, is responsible for import and export of glucose in red blood cells. However, knowledge on how chronic stress impact glucose and GLUT 1 protein in fish subjected to HSD is still unclear. In this study, effect of HSD on the expression of GLUT 1 in Nile tilapia was investigated in an attempt to elucidate the role of GLUT 1 in glucose metabolism during chronic stress. Fish were reared for 4 weeks at 1.5 and 4.5 kg/m 3 for low stocking density (LSD) and HSD, respectively. Four physiological parameters were determined from the blood samples obtained from fish at the end of experiment. At p \ 0.05, there were significant differences between fish reared at HSD and LSD in plasma cortisol level (72.1 ± 5.9 ng/ml and 37.5 ± 4.6 ng/ml); blood glucose level (136.00 ± 1.3 mg/dL and 70.2 ± 1.0 mg/dL); erythrocytes count (7.2 ± 0.5 9 106 mm -3 and 2.1 ± 0.4 9 106 mm -3 ); and plasma GLUT 1 level (1.40 ± 0.17 rbi and 0.81 ± 0.07 rbi), respectively. HSD induced elevation of plasma cortisol level, blood glucose level, erythrocytes count and GLUT 1 level. These elevated physiological factors and particularly GLUT 1 can be used as a cellular stress biomarker in fish farming and aquaculture.

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“…Amar et al (2000) observaram aumento da resposta imune de trutas alimentadas com ração suplementada com ß-caroteno. As vitaminas C e E são consideradas moderadoras de estresse em peixes (MERCHIE et al, 1997;MONTERO et al, 2001;KOLKOVSKI et al, 2000;ORTUÑO et al, 2003).…”

Section: Introductionunclassified

Licopeno no bem-estar de juvenis de tilápia do Nilo (Oreochromis niloticus): efeito sobre desempenho e parâmetros bioquímicos

Oliveira¹

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AGRADECIMENTOSÀ Deus, por tudo... À minha orientadora, Profa. Dra. Mariza Pires de Melo, pela acolhida, paciência, empenho e dedicação; à minha co-orientadora Profa. Dra. Elyara Maria Pereira-daSilva, pela ajuda e acolhida e as duas por acreditar em mim além da amizade, Aos meus pais e irmã, que continuaram acreditando e me apoiando neste novo desafio, Ao Paulitico, meu namorado que acabou esposo no decorrer desta tese, obrigada pela compreensão e cumplicidade em todos os momentos, pela ajuda nas horas precisas e por torcer por mim e a sua e agora minha família, pelos bons momentos compartilhados, A família da minha saudosa amiga, Adriana A. Cuel Barone, Dri, agora minhas novas famílias, que tive a sorte de conquistar aqui em Pirassununga, Cuel -Assis - ABSTRACTThe aim of this study was to evaluate the hypothesis that the inclusion of lycopene in the commercial diet reduces the negative effects of stress and improves performance of juvenile Nile tilapia, Oreochromis niloticus. To this end was accomplished, first, a study that evaluated the effect of supplementation with lycopene (100, 200, 400 and 800 mg/kg of diet) on the attraction and palatability of the diet. In a second step, the effect of daily supplementation of lycopene (800 mg/kg of diet) for 60 days, to promote the welfare of juveniles subjected to restraint stress for 90 minutes.Zootechnical parameters were evaluated as weight gain, feed conversion, condition factor and specific growth rate, consumption and physiological and biochemical parameters as the plasma cortisol level, the content of lipid peroxidation, instrumental color of the fillet and the activity of antioxidant enzymes catalase (CAT) and glutathione reductase (GR) and activity of lactate dehydrogenase (LDH) in white muscle (fillet) and red muscle. The results were submitted to ANOVA and in case of significant effect between treatments, means were compared by Tukey test (p<0.05).The inclusion of lycopene did not affect the attraction and palatability of commercial feed in any of the concentrations studied. Supplementation with lycopene (800 mg/kg diet) did not alter any of the zootechnical parameters evaluated: weight gain, feed conversion, condition factor and specific growth rate. Likewise, did not alter the level of lipid peroxidation evaluated by thiobarbituric acid reactive substance (TBARS) and instrumental color of the fillet of fish. There was a reduction (34%) in catalase activity in white muscle of fish supplemented with lycopene, compared to the control group (no supplementation). However, catalase activity in red muscle was not altered by supplementation with lycopene, as well as GR activity and LDH in white muscle and red. It was demonstrated that restraint stress increased the plasma cortisol level (53%), level of lipid peroxidation of the fillet (28%) and activity of CAT, GR and LDH in white muscle (31%, 32% and 29%, respectively) and red muscle (48%, 46% and 44%, respectively) of animals subjected to stress. However, dietary supplementation with lycopene abolished the neg...

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Low vitamin E in diet reduces stress resistance of gilthead seabream (Sparus aurata) juveniles

Cited by 130 publications

References 50 publications

Haematological and Immunological Responses in Juvenile Sea Bass (Dicentrarchus labrax L.) After Short-Term Acute Stress

Haematological and Immunological Responses in Juvenile Sea Bass (Dicentrarchus labrax L.) After Short-Term Acute Stress

Effect of stocking density on the expression of glucose transporter protein 1 and other physiological factors in the Lake Victoria Nile tilapia, Oreochromis niloticus (L.)

Licopeno no bem-estar de juvenis de tilápia do Nilo (Oreochromis niloticus): efeito sobre desempenho e parâmetros bioquímicos

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