Low-voltage lab-on-chip for micro and nanoparticles manipulation and detection: experimental results

IEEE Trans. Biomed. Circuits Syst.

Auclair²,

Srasra³

2015

Self Cite

In this paper, we present a new rapid prototyping platform dedicated to dielectrophoretic microfluidic manipulation and capacitive cell sensing. The proposed platform offers a reconfigurable design including 4 independently programmable output channels to be distributed across 64 electrodes. Although its range of frequency covers up to 3.4 MHz, signal amplitude accuracy ( +/-10%) was demonstrated for frequencies up to 1 MHz and channel-to-channel phase shift setting was stable up to 1.5 MHz. A test of maximum resistive load showed a 10% attenuation of a 12 V peak-to-peak signal with a 22 Ω load. The platform has an advanced capacitive sensor to measure capacitance variation between in-channel electrodes with a sampling frequency up to 5 kH z. Experimental data of capacitive sensor showed a sensitivity of 100 fF. The sensor can be extended to 4 parallel measurements with lower frequency. We also present a new assembly technique for reusable microfluidic chip based on anisotropic adhesive conductive film, epoxy and PDMS. The proposed platform provides a wide range of control signals depending on the type of manipulation as sine, rectangular or square wave. The frequency range is extendible up to 3.4 MHz, in addition to a programmable phase shift circuit with a minimum phase step of 3.6(°) for each signal.

mentioning

confidence: 55%

mentioning

confidence: 55%

Reconfigurable Prototyping Microfluidic Platform for DEP Manipulation and Capacitive Sensing

IEEE Trans. Biomed. Circuits Syst.

Auclair²,

Srasra³

2015

Self Cite

“…, 4 and 8 electrodes for the first and second presented architectures respectively [33,35]. The latter cannot provide the exact 3D position of particles in the micro-channel.…”

Section: Simulation and Experimental Resultsmentioning

confidence: 99%

Hybrid Modeling Method for a DEP Based Particle Manipulation

Gagne

2013

Sensors

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In this paper, a new modeling approach for Dielectrophoresis (DEP) based particle manipulation is presented. The proposed method fulfills missing links in finite element modeling between the multiphysic simulation and the biological behavior. This technique is amongst the first steps to develop a more complex platform covering several types of manipulations such as magnetophoresis and optics. The modeling approach is based on a hybrid interface using both ANSYS and MATLAB to link the propagation of the electrical field in the micro-channel to the particle motion. ANSYS is used to simulate the electrical propagation while MATLAB interprets the results to calculate cell displacement and send the new information to ANSYS for another turn. The beta version of the proposed technique takes into account particle shape, weight and its electrical properties. First obtained results are coherent with experimental results.

“…We developed an LoC architecture and we validated the functionality of achieved device with 15 µm algae cells. The proposed LoC can be used for different applications as shown in our previous works [6], [7]. In this paper our emphasis is more particularly on fresh water monitoring.…”

Section: Introductionmentioning

confidence: 94%

Reconfigurable Lab-on-Chip platform for algae cell manipulation

2014 IEEE International Symposium on Circuits and Systems (ISCAS)

2014

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In this paper, we present a new dielectrophoretic microfluidic technique in a modular Lab-on-Chip (LoC) platform. The proposed LoC has a reconfigurable topology. It generates a wide range of signals depending on the analyzed liquid with a variable frequency up to 1.2 MHz. In addition, a programmable phase shift circuit with a minimum phase step of 3.6 • for each signal is implemented. The amplitude of each signal can be adjusted independently, and the latter can be distributed through 64 bidirectional electrodes. Each electrode can be enabled or disabled individually. Moreover, device includes capacitive sensing stage for the measurement of the in-channel capacitance change induced by algae. Furthermore, The architecture of the proposed system is versatile and can be adjusted to different cell manipulation applications. The presented LoC was tested with 15 µm algae cells.