Lower expression of sialic acid receptors in the cecum of silky fowl (Gallus gallus domesticus Brisson) compared to white leghorn

Han, Deguo; Hu, Yanxin; Teng, Ke; Deng, Xuemei

doi:10.3382/ps/pew065

Cited by 8 publications

(10 citation statements)

References 25 publications

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“…However, in the present study, the reduced expression of genes was related to the Toll-like and NOD-like receptor pathways, which might result from the proliferation, rather than the differentiation, of B-cells in the BF. Our recent studies also demonstrated that a few immune cells in the cecum of SF were closely related to the reduced expression of sialic acid receptors 27 .…”

Section: Discussionmentioning

confidence: 61%

Transcriptome analyses of differential gene expression in the bursa of Fabricius between Silky Fowl and White Leghorn

Han

Zhang

Chen

et al. 2017

Sci Rep

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Hyperpigmentation in Silky Fowl (SF) results in aberrant immune cell development. However, how melanocytes regulate B-cell proliferation in the bursa of Fabricius (BF) is unclear. To resolve this conundrum, we collected BFs from three-week-old SF and White Leghorn (WL) female chickens for RNA sequencing. The BF development was relatively weaker in SF than in WL. The transcriptome analyses identified 4848 differentially expressed genes, 326 long noncoding RNAs (lncRNAs), and 67 microRNAs in the BF of SF. The genes associated with melanogenesis was significantly higher, but that of the genes associated with the cytokine-cytokine receptor interactions and JAK-STAT signalling pathway was significantly lower in SF than in WL. Crucial biological processes, such as the receptor activity, cell communication, and cellular responses to stimuli, were clustered in SF. The predicted target lncRNAs genes were mainly associated with cell proliferation pathways such as JAK-STAT, WNT, MAPK, and Notch signalling pathways. Except for the above pathways, the target microRNA genes were related to the metabolism, melanogenesis, autophagy, and NOD-like and Toll-like receptor signalling pathways. The lncRNAs and microRNAs were predicted to regulate the JAK2, STAT3, and IL-15 genes. Thus, B-cell development in the BF of SF might be regulated and affected by noncoding RNAs.

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Section: Discussionmentioning

confidence: 61%

Transcriptome analyses of differential gene expression in the bursa of Fabricius between Silky Fowl and White Leghorn

Han

Zhang

Chen

et al. 2017

Sci Rep

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“…Macrophages express Gal-specific receptors (39), which could explain the higher abundance of SAα2,3Gal in the cecum of infected birds relative to controls. Evidence of macrophages expressing Gal-specific receptors are seen in white leghorn chickens, which in one study had a greater abundance of sialic acid receptors than silky fowl because of a higher number of immune cells in the leghorns' cecum (40). The cecum has a unique response to LPAIV infection compared to other intestinal tissues, which warrants further analysis of SAα2,3Gal in this tissue.…”

Section: Two Hypotheses Could Explain the Negative Relationship Betweenmentioning

confidence: 94%

The Association Between SAα2,3Gal Occurrence Frequency and Avian Influenza Viral Load in Mallards (Anas Platyrhynchos) and Blue-Winged Teals (Spatula discors)

Doliński

Jankowski²,

Fair

et al. 2020

Preprint

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Background: Individual heterogeneity in pathogen load can affect disease transmission dynamics; therefore, identifying intrinsic factors responsible for variation in pathogen load is necessary for determining which individuals are prone to be most infectious. Because low pathogenic avian influenza viruses (LPAIV) preferentially bind to alpha-2,3 sialic acid receptors (SAα2,3Gal) in the intestines and bursa of Fabricius in wild ducks (Anas and Spatula spp.), we investigated juvenile mallards (Anas platyrhyncos) and blue-winged teals (Anas discors) orally inoculated with A/northern pintail/California/44221-761/2006 (H5N9) and the virus titer relationship to occurrence frequency of SAα2,3Gal in the intestines and bursa. To test the natural variation of free-ranging duck populations, birds were hatched and raised in captivity from eggs collected from nests of free-ranging birds in North Dakota, USA. Data generated from qPCR were used to quantify virus titers in cloacal swabs, ileum tissue, and bursa of Fabricius tissue, and lectin histochemistry was used to quantify the occurrence frequency of SAα2,3Gal. Linear mixed models were used to analyze infection status, species, and sex-based differences. Multiple linear regression was used to analyze the relationship between virus titer and SAα2,3Gal occurrence frequency. Results: In mallards, we found high individual variation in virus titers significantly related to high variation of SAα2,3Gal in the ileum. In contrast to mallards, individual variation in teals was minimal and significant relationships between virus titers and SAα2,3Gal were not determined. Collectively, teals had both higher virus titers and a higher occurrence frequency of SAα2,3Gal compared to mallards, which may indicate a positive association between viral load and SAα2,3Gal. Statistically significant differences were observed between infected and control birds indicating that LPAIV infection may influence the occurrence frequency of SAα2,3Gal, or vice versa, but only in specific tissues. Conclusions: The results of this study provide quantitative evidence that SAα2,3Gal abundance is related to LPAIV titers; thus, SAα2,3Gal should be considered a potential intrinsic factor influencing variation in LPAIV load.

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“…Macrophages express Galspecific receptors [38], which could explain the higher abundance of SAα2,3Gal in the cecum of infected birds relative to controls. Evidence of macrophages expressing Gal-specific receptors are seen in white leghorn chickens, which in one study had a greater abundance of sialic acid receptors than silky fowl because of a higher number of immune cells in the leghorns' cecum [39]. The cecum has a unique response to LPAIV infection compared to other intestinal tissues, which warrants further analysis of SAα2,3Gal in this tissue.…”

Section: Discussionmentioning

confidence: 94%

The association between SAα2,3Gal occurrence frequency and avian influenza viral load in mallards (Anas platyrhynchos) and blue-winged teals (Spatula discors)

et al. 2020

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Background Individual heterogeneity in pathogen load can affect disease transmission dynamics; therefore, identifying intrinsic factors responsible for variation in pathogen load is necessary for determining which individuals are prone to be most infectious. Because low pathogenic avian influenza viruses (LPAIV) preferentially bind to alpha-2,3 sialic acid receptors (SAα2,3Gal) in the intestines and bursa of Fabricius in wild ducks (Anas and Spatula spp.), we investigated juvenile mallards (Anas platyrhyncos) and blue-winged teals (Anas discors) orally inoculated with A/northern pintail/California/44221–761/2006 (H5N9) and the virus titer relationship to occurrence frequency of SAα2,3Gal in the intestines and bursa. To test the natural variation of free-ranging duck populations, birds were hatched and raised in captivity from eggs collected from nests of free-ranging birds in North Dakota, USA. Data generated from qPCR were used to quantify virus titers in cloacal swabs, ileum tissue, and bursa of Fabricius tissue, and lectin histochemistry was used to quantify the occurrence frequency of SAα2,3Gal. Linear mixed models were used to analyze infection status, species, and sex-based differences. Multiple linear regression was used to analyze the relationship between virus titer and SAα2,3Gal occurrence frequency. Results In mallards, we found high individual variation in virus titers significantly related to high variation of SAα2,3Gal in the ileum. In contrast to mallards, individual variation in teals was minimal and significant relationships between virus titers and SAα2,3Gal were not determined. Collectively, teals had both higher virus titers and a higher occurrence frequency of SAα2,3Gal compared to mallards, which may indicate a positive association between viral load and SAα2,3Gal. Statistically significant differences were observed between infected and control birds indicating that LPAIV infection may influence the occurrence frequency of SAα2,3Gal, or vice versa, but only in specific tissues. Conclusions The results of this study provide quantitative evidence that SAα2,3Gal abundance is related to LPAIV titers; thus, SAα2,3Gal should be considered a potential intrinsic factor influencing variation in LPAIV load.

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Lower expression of sialic acid receptors in the cecum of silky fowl (Gallus gallus domesticus Brisson) compared to white leghorn

Cited by 8 publications

References 25 publications

Transcriptome analyses of differential gene expression in the bursa of Fabricius between Silky Fowl and White Leghorn

Transcriptome analyses of differential gene expression in the bursa of Fabricius between Silky Fowl and White Leghorn

The Association Between SAα2,3Gal Occurrence Frequency and Avian Influenza Viral Load in Mallards (Anas Platyrhynchos) and Blue-Winged Teals (Spatula discors)

The association between SAα2,3Gal occurrence frequency and avian influenza viral load in mallards (Anas platyrhynchos) and blue-winged teals (Spatula discors)

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