Lower fluidity of supported lipid bilayers promotes neuronal differentiation of neural stem cells by enhancing focal adhesion formation

Hao, Wangping; Han, Jie; Chu, Yun; Huang, Lei; Zhuang, Yan; Li, Xiaoran; Ma, Hongwei; Chen, Yanyan; Dai, Jianwu

doi:10.1016/j.biomaterials.2018.01.034

Cited by 14 publications

(14 citation statements)

References 44 publications

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“…8 c, d). Vinculin is a highly conserved actin-binding protein that is frequently used as a marker for focal adhesion [ 61 , 62 ]. Vinculin was stained to reveal the cell adhesion (Fig.…”

Section: Resultsmentioning

confidence: 99%

Mussel-Inspired Redox-Active and Hydrophilic Conductive Polymer Nanoparticles for Adhesive Hydrogel Bioelectronics

et al. 2020

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Conductive polymers (CPs) are generally insoluble, and developing hydrophilic CPs is significant to broaden the applications of CPs. In this work, a mussel-inspired strategy was proposed to construct hydrophilic CP nanoparticles (CP NPs), while endowing the CP NPs with redox activity and biocompatibility. This is a universal strategy applicable for a series of CPs, including polyaniline, polypyrrole, and poly(3,4-ethylenedioxythiophene). The catechol/quinone contained sulfonated lignin (LS) was doped into various CPs to form CP/LS NPs with hydrophilicity, conductivity, and redox activity. These CP/LS NPs were used as versatile nanofillers to prepare the conductive hydrogels with long-term adhesiveness. The CP/LS NPs-incorporated hydrogels have a good conductivity because of the uniform distribution of the hydrophilic NPs in the hydrogel network, forming a well-connected electric path. The hydrogel exhibits long-term adhesiveness, which is attributed to the mussel-inspired dynamic redox balance of catechol/quinone groups on the CP/LS NPs. This conductive and adhesive hydrogel shows good electroactivity and biocompatibility and therefore has broad applications in electrostimulation of tissue regeneration and implantable bioelectronics.

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“…8 c, d). Vinculin is a highly conserved actin-binding protein that is frequently used as a marker for focal adhesion [ 61 , 62 ]. Vinculin was stained to reveal the cell adhesion (Fig.…”

Section: Resultsmentioning

confidence: 99%

Mussel-Inspired Redox-Active and Hydrophilic Conductive Polymer Nanoparticles for Adhesive Hydrogel Bioelectronics

et al. 2020

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“…The FAK‐MEK/ERK signaling pathway can be subsequently activated and induce neuronal differentiation. [ 48 ] In addition, Cho and co‐workers reported that expression of a voltage‐gated Ca 2+ channel in a neural stem/progenitor cell increased on conductive hydrogels made of carbon nanotubes and hyaluronic acid gels. [ 45 ] The authors suggested that influx of a large amount of Ca 2+ into cells through Ca 2+ channels can activate several signaling pathways involved in neuronal differentiation.…”

Section: Discussionmentioning

confidence: 99%

Electrically Conductive Hydrogel Nerve Guidance Conduits for Peripheral Nerve Regeneration

Park

Jeon

Kim

et al. 2020

Adv Funct Materials

169

111

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Peripheral nerve injuries are serious conditions, and surgical treatment has critical limitations. Therefore, nerve guidance conduits (NGCs) are proposed as an alternative. In this study, multifunctional NGCs are fabricated for the regeneration of injured peripheral nerves. Graphene oxide (GO) and gelatin-methacrylate (GelMA) are polymerized and chemically reduced to form reduced (GO/GelMA) (r(GO/GelMA)). The prepared materials present good electrical conductivity, flexibility, mechanical stability, and permeability, which are suitable for use as NGCs. In vitro studies show 2.1-and 1.4-fold promotion of neuritogenesis of PC12 neuronal cells on r(GO/GelMA) compared to GelMA and unreduced GO/GelMA, respectively. Animal studies using a rat sciatic nerve injury model with a 10 mm gap between the proximal and distal regions of the defect reveal that r(GO/GelMA) NGCs significantly enhance peripheral nerve regeneration, indicated by improved muscle weight increase, electro-conduction velocity, and sciatic nerve function index. Specifically, r(GO/GelMA) NGCs are utilized to potentiate regrowth with myelination in rat sciatic nerves followed by histological, immunohistological, and morphometrical analyses. This study successfully shows the feasibility of electrically conductive hydrogel NGCs as functional conduits for improved nerve regeneration in a preclinical study, where these NGCs can not only mimic nerve tissues but also strongly promote nerve regeneration.

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“…Hao et al reported the effect of the mechanical properties of an ECM-SLB on the differentiation of neural stem cells (NSCs) (Hao et al, 2018). SLBs were used to as a culture platform to mimic the dynamic characteristics of the fluid ECM.…”

Section: Extracellular Matrix-supported Lipid Bilayer Systemsmentioning

confidence: 99%

Construction of Cell–Extracellular Matrix Microenvironments by Conjugating ECM Proteins on Supported Lipid Bilayers

Huang

2019

Front. Mater.

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The cell membrane is an organized and fluid structure that modulates cellular activities in response to specific extracellular signals, and maintains the critical communication, integration, and homeostasis between the cytosol and the extracellular matrix (ECM). In recent years, tissue engineering and cell biology research has been rapidly progressed by a remarkable understanding of cell and ECM interfaces. In this review, the design of new biomimetic platforms based on the conjugation of ECM proteins on solid supported lipid bilayers (SLBs) will be summarized. The platforms provide a better system to evaluate cellular responses to specific recognition events, gradient, mechanical property, nanostructures, and inter-and intra-molecular interactions of ECM proteins on a non-fouling and fluid membrane. Moreover, the findings from the molecular interactions and cellular activities will be highlighted to look into the cell-materials mechanisms.

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Lower fluidity of supported lipid bilayers promotes neuronal differentiation of neural stem cells by enhancing focal adhesion formation

Cited by 14 publications

References 44 publications

Mussel-Inspired Redox-Active and Hydrophilic Conductive Polymer Nanoparticles for Adhesive Hydrogel Bioelectronics

Mussel-Inspired Redox-Active and Hydrophilic Conductive Polymer Nanoparticles for Adhesive Hydrogel Bioelectronics

Electrically Conductive Hydrogel Nerve Guidance Conduits for Peripheral Nerve Regeneration

Construction of Cell–Extracellular Matrix Microenvironments by Conjugating ECM Proteins on Supported Lipid Bilayers

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