LOX-1 receptor: A potential link in atherosclerosis and cancer

Balzan, Silvana; Lubrano, Valter

doi:10.1016/j.lfs.2018.02.024

Cited by 114 publications

(92 citation statements)

References 109 publications

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“…These markers promote the influx and activation of other inflammatory cells, as T‐lymphocytes, and mediate their retention in the plaque that increases the inflammatory process around it . Besides that, the NO induces high production of peroxynitrite and consequent cell toxicity …”

Section: Discussionsupporting

confidence: 81%

“…[60,61] Besides that, the NO induces high production of peroxynitrite and consequent cell toxicity. [62] DTP and digested glutelin were the most effective to reduce the expression of NF-κB and iNOS, and the secretion of TNF-α, PGE-2, MCP-1, and IL-6, which are the inflammatory markers in the atherosclerosis process. These results showed the effectiveness of digested protein from chia seed, like others studies with bean hydrolysates [43] and extruded amaranth hydrolysate.…”

Section: Discussionmentioning

confidence: 98%

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Chia (Salvia hispanica L.) Seed Total Protein and Protein Fractions Digests Reduce Biomarkers of Inflammation and Atherosclerosis in Macrophages In Vitro

Grancieri

Martino

Mejía

2019

Molecular Nutrition Food Res

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Scope The objectives are to evaluate the anti‐inflammatory and anti‐atherosclerotic effects of digested total protein and digested protein fractions from chia seed in macrophages in vitro. Methods and results Total protein and protein fractions (albumin, globulin, glutelin, and prolamin) are isolated from chia seed and digested using simulated gastrointestinal conditions, resulting in digested total protein (DTP) and digested protein fractions (DPF). DTP and DPF are applied (1.0 mg mL−1) in RAW 264.4 macrophages stimulated with LPS (1 µg mL−1) for inflammation or ox‐LDL (80 µg mL−1) for atherosclerosis. In the inflammatory process, DTP and DPF reduce p‐NF‐κB, iNOS, p‐JNK, and AP‐1. Digested glutelin reduces the secretion of nitric oxide (65.1%), reactive oxygen species (19.7%), prostaglandins (34.6%), TNF‐α (24.1%), MCP‐1 (18.9%), IL‐6 (39.6%), and IL‐10 (68.7%). DTP and DPF reduce the NF‐κB translocation to nuclei. DTP and digested glutelin reduce iCAM expression (86.4%, 80.8%), LOX‐1 (37.3%, 35.7%), iNOS (67.0%, 42.2%), and NF‐κB (57.5%, 71.1%). DTP is effective in reducing secretion of nitric oxide (43.4%), lipid accumulation (41.9%), prostaglandins (41.9%), TNF‐α (43.3%), MCP‐1 (47.6%), and IL‐6 (50.5%). Peptides from chia DTP and DPF are also characterized. Conclusion DTP and digested glutelin from chia seed reduce expression and secretion of markers related to inflammation and atherosclerosis pathways.

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Section: Discussionsupporting

confidence: 81%

Section: Discussionmentioning

confidence: 98%

Chia (Salvia hispanica L.) Seed Total Protein and Protein Fractions Digests Reduce Biomarkers of Inflammation and Atherosclerosis in Macrophages In Vitro

Grancieri

Martino

Mejía

2019

Molecular Nutrition Food Res

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“…However, above the dose of 10 ng/mL, TNF-α reduced about 10% of cell viability (Figure 1a). Because MMPs break down components of ECM, which is a crucial role in the process of VSMC migration [7,8], the effect of TNF-α on MMP activities was then tested by gelatin zymography in serum-free conditioned medium to identify the contribution of MMP-2 or MMP-9 to the pro-migratory ability of TNF-α. As shown in Figure 1b, MMP-9 activity was tremendously increased by TNF-α in a concentration-dependent manner, whereas MMP-2 activity was less affected.…”

Section: Non-cytotoxic Doses Of Hlp Inhibits Tnf-α-induced Cell Viabimentioning

confidence: 99%

Anti-Atherosclerotic Effect of Hibiscus Leaf Polyphenols against Tumor Necrosis Factor-alpha-Induced Abnormal Vascular Smooth Muscle Cell Migration and Proliferation

Chou¹,

Wang

Chen

et al. 2019

Antioxidants

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The proliferation and migration of vascular smooth muscle cells (VSMCs) are major events in the development of atherosclerosis following stimulation with proinflammatory cytokines, especially tumor necrosis factor-alpha (TNF-α). Plant-derived polyphenols have attracted considerable attention in the prevention of atherosclerosis. Hibiscus leaf has been showed to inhibit endothelial cell oxidative injury, low-density lipoprotein oxidation, and foam cell formation. In this study, we examined the anti-atherosclerotic effect of Hibiscus leaf polyphenols (HLPs) against abnormal VSMC migration and proliferation in vitro and in vivo. Firstly, VSMC A7r5 cells pretreated with TNF-α were demonstrated to trigger abnormal proliferation and affect matrix metalloproteinase (MMP) activities. Non-cytotoxic doses of HLPs abolished the TNF-α-induced MMP-9 expression and cell migration via inhibiting the protein kinase PKB (also known as Akt)/activator protein-1 (AP-1) pathway. On the other hand, HLP-mediated cell cycle G0/G1 arrest might be exerted by inducing the expressions of p53 and its downstream factors that, in turn, suppress cyclin E/cdk2 activity, preventing retinoblastoma (Rb) phosphorylation and the subsequent dissociation of Rb/E2F complex. HLPs also attenuated reactive oxygen species (ROS) production against TNF-α stimulation. In vivo, HLPs improved atherosclerotic lesions, and abnormal VSMC migration and proliferation. Our data present the first evidence of HLPs as an inhibitor of VSMC dysfunction, and provide a new mechanism for its anti-atherosclerotic activity.

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“…A constitutive expression of LOX-1 is observed in vascular endothelial cells in the physiological state. In addition, macrophages and platelets show inducible LOX-1 expression stimulated by inflammatory cytokines as a result of ischemia and vascular injury [18]. The binding of several ligands to LOX-1 induces superoxide generation, inhibits NO production, and enhances the endothelial adhesiveness of leukocytes.…”

Section: Discussionmentioning

confidence: 99%

Increased Tissue Expression of Lectin-Like Oxidized LDL Receptor-1 (LOX-1) Is Associated with Disease Severity in Chronic Rhinosinusitis with Nasal Polyps

et al. 2020

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Background: The oxidative stress, induced by both environmental and intrinsic stimuli, underlies the onset and persistency of chronic rhinosinusitis (CRS). Scavenger receptors (SRs) are a broad family of transmembrane receptors involved in a dysfunctional host–environment interaction through a reaction with reactive oxygen species (ROS) production. Objective: We hypothesized possible roles of two major SRs in CRS pathology that can translate to clinical phenotypes or histological subtypes: lectin-like oxidized low-density lipoproteins (LDL) receptor-1 (LOX-1) and scavenger receptor class B type 1 (SR-B1). Patients and Methods: We collected ethmoid sinus mucosa specimens and blood samples from patients with CRS with nasal polyps (CRSwNP; n = 31) or CRS without NP (CRSsNP; n = 13) and 19 control subjects. We performed an RT-PCR analysis, ELISA assay, and immunostaining to determine the expressions and distributions of LOX-1 and SR-B1. Results: The CRSwNP group showed a significant increase in LOX-1 mRNA expression compared to the control group. There was no significant difference in SR-B1 mRNA levels among the three groups. The LOX-1 mRNA levels were positively correlated with the sinus computed tomography (CT) scores. Sinus tissue, but not serum samples, showed elevated concentrations of LOX-1 protein in the CRSwNP group versus the control group. The LOX-1 protein distribution was localized in inflammatory cells and vascular endothelial cells. Conclusion: LOX-1 is a major receptor for oxidized low-density lipoprotein produced by oxidative stress. This is the first study to report alterations in LOX-1 expression and production triggered by persistent inflammatory processes in CRSwNP patients. Our findings reveal complex but important roles for SRs that may contribute to the onset of different CRS phenotypes.

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LOX-1 receptor: A potential link in atherosclerosis and cancer

Cited by 114 publications

References 109 publications

Chia (Salvia hispanica L.) Seed Total Protein and Protein Fractions Digests Reduce Biomarkers of Inflammation and Atherosclerosis in Macrophages In Vitro

Chia (Salvia hispanica L.) Seed Total Protein and Protein Fractions Digests Reduce Biomarkers of Inflammation and Atherosclerosis in Macrophages In Vitro

Anti-Atherosclerotic Effect of Hibiscus Leaf Polyphenols against Tumor Necrosis Factor-alpha-Induced Abnormal Vascular Smooth Muscle Cell Migration and Proliferation

Increased Tissue Expression of Lectin-Like Oxidized LDL Receptor-1 (LOX-1) Is Associated with Disease Severity in Chronic Rhinosinusitis with Nasal Polyps

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