Lox-dependent gene expression in transgenic plants obtained via Agrobacterium-mediated transformation

Shcherbak, N. L.; Kishchenko, Olena; Sakhno, L. O.; Komarnytsky, I. K.; Кучук, М. В.

doi:10.3103/s0095452713030079

Cited by 3 publications

(3 citation statements)

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“…Agrobacterium -mediated transformation of lettuce ‘Odeskyj curling’; with the vector pNMD46772 containing the cma gene was carried out as described in Shcherbak et al. (2013) .…”

Section: Methodsmentioning

confidence: 99%

“…Agrobacterium-mediated transformation of lettuce 'Odeskyj curling'; with the vector pNMD46772 containing the cma gene was carried out as described in Shcherbak et al (2013). After 48 h of co-cultivation with diluted Agrobacterium suspension supplemented with 100 mM acetosyringone, explants were placed on modified B5 (Gamborg et al, 1968) regeneration medium (B5 medium salts with 2.5% sucrose, 3 mg/L kinetin, 0.5 mg/L NAA) supplemented with 5 mg/L phosfinothricin (PPT) for selection.…”

Section: Plant Transformation and Regenerationmentioning

confidence: 99%

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Accumulation of colicin M protein and its biological activity in transgenic lettuce and mizuna plants

Shcherbak,

Prochaska,

Lystvan

et al. 2023

Front. Plant Sci.

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Food-borne illnesses caused by pathogenic Escherichia coli strains, especially enterohaemorrhagic E. coli (EHEC), are a serious public health problem, as debilitating disease and even death from such food poisonings have been repeatedly reported. Colicin M (ColM), a non-antibiotic antimicrobial protein produced by some strains of E. coli, has shown promising activity in controlling multiple enteropathogenic strains of E. coli and related pathogens. As contaminated green leafy vegetables are a frequent source of pathogenic E. coli infections, we genetically modified (GM) two edible crops, lettuce (Lactuca sativa L.) and mizuna (Brassica rapa subsp. nipposinica var. laciniata), to stably express the ColM gene and assessed the antibacterial activity of tissue extracts from these plants against selected E. coli strains in vitro. Transgenic plants of these species were developed using Agrobacterium-mediated transformation with a vector containing the ColM-coding gene (cma) under the control of the 35S promoter. Western blot analysis of recombinant ColM protein was performed in selected transgenic plants to confirm cma gene expression and quantify ColM accumulation. Extracts of transgenic plants expressing ColM showed significant activity against two major strains of EHEC (O157:H7 and O104:H4) as well as E. coli strains resistant to beta-lactam- and carbapenem-class antibiotics. Importantly, the antibacterial activity persisted in several subsequent generations of transgenic lettuce and mizuna plants that stably expressed the ColM gene. In addition, our results also show that the antibacterial activity of dried (up to 40°C) biomass of transgenic plants remained stable without a decrease for at least three months.

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“…Agrobacterium -mediated transformation of lettuce ‘Odeskyj curling’; with the vector pNMD46772 containing the cma gene was carried out as described in Shcherbak et al. (2013) .…”

Section: Methodsmentioning

confidence: 99%

Section: Plant Transformation and Regenerationmentioning

confidence: 99%

Accumulation of colicin M protein and its biological activity in transgenic lettuce and mizuna plants

Shcherbak,

Prochaska,

Lystvan

et al. 2023

Front. Plant Sci.

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“…Such genes hardly expressed in the same position when the genetic constructs were transferred into plants via bombardment. The phenomenon of lox-mediated expression was observed in a number the transgenic species, such as African tobacco, canola, lettuce, potato, and sugar beets [21,25,26].…”

mentioning

confidence: 99%

Cell genetic engineering: Transmission genetics of plants

Кучук

2017

Cytol. Genet.

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Abstract⎯Achievements of cell and genetic engineering that led to formation of a new genetics chaptertransmission genetics-have been described. Results have been analyzed and new opportunities in the field of transgenomic somatic hybrids and cybrid obtaining, production of transgenic plants with agronomic pharmaceutical application, development of transplastomic plants, and accumulation of recombinant proteins by using the transient expression of foreign genes in plants have been shown.

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Antioxidant activity in Brassica napus L. plants expressing lox-dependent BAR gene

Sakhno¹,

Lystvan²

1970

Fakt. eksp. evol. org.

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Aim. The study of possible unintended biochemical peculiarities of newly obtained herbicide-resistant canola (Brassica napus L.) plants expressing the lox-dependent BAR gene was the aim. Methods. Total soluble protein content, total free radical scavenging activity, and superoxide dismutase activity have been investigated using Bradford’s, DPPH, and nitroblue tetrazolium assays, respectively. Fresh weight of plants grown on media with or without phosphinothricin was also measured. Results. The antioxidant activity of leaf extracts of untransformed plants under in vitro growth condi-tions had no significant differences in comparison with ones of phospinothricin-resistant plants in the third generation. No significant changes in parameters investigated were observed in transgenic plants cultivated on media with herbicide addition compared to ones grown on media without it. Fresh weight and total soluble protein content were similar in transgenic and untransformed canola plants under growth without phosphinothricin. Conclusions. Lox-dependent BAR gene introduction and expression resulted in no significant differences in leaf antioxidant activity in transgenic canola plants comparing to untransformed controls.Keywords: Brassica napus, antioxidant activity, DPPH, glufosinate, superoxide dismutase.

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Lox-dependent gene expression in transgenic plants obtained via Agrobacterium-mediated transformation

Cited by 3 publications

References 48 publications

Accumulation of colicin M protein and its biological activity in transgenic lettuce and mizuna plants

Accumulation of colicin M protein and its biological activity in transgenic lettuce and mizuna plants

Cell genetic engineering: Transmission genetics of plants

Antioxidant activity in Brassica napus L. plants expressing lox-dependent BAR gene

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