Lpar2b Controls Lateral Line Tissue Size by Regulating Yap1 Activity in Zebrafish

Abstract

LPA signaling plays important roles during cell migration and proliferation in normal and pathological conditions. However, its role during sensory organ development remains unknown. Here we show a LPA receptor Lpar2b is expressed in the posterior lateral line primordium (pLLP) and mechanosensory organs called neuromasts (NMs) in zebrafish embryos. Lpar2b loss-of-function significantly reduces the number of NMs and hair cells in the posterior lateral line (pLL). Further analysis reveals that Lpar2b regulates the patterning and tissue size of the pLLP. Interestingly, we show that knocking down a Hippo effector Yap1 phenocopies the result of Lpar2b depletion, and Lpar2b regulates the phosphorylation and activity of Yap1 in the pLLP. Importantly, a phosphorylation-resistant Yap1 rescues pLLP size and NM number in Lpar2b-depleted embryos. Our results indicate Lpar2b controls primordium size and NM number by regulating Yap1 activity in the lateral line system.

Role of Hippo-YAP Signaling in Osseointegration by Regulating Osteogenesis, Angiogenesis, and Osteoimmunology

“…What’s more, some extracellular lipids or hormone signals can bond to G-protein-coupled receptors (GPCRs) and regulates Hippo-YAP signaling mediated by F-actin signaling ( Moya and Halder, 2019 ). Based on a series of researches on the crosstalk between lysophosphatidic acid (LPA) and Hippo-YAP signaling, it is suggested that LPA is an upstream signal of Hippo pathway, binding to GPCRs and regulating the activity of the downstream effector YAP, which further mediates gene expression and cell behaviors ( Yu et al, 2012 ; Moroishi et al, 2015 ; Park et al, 2016 ; Wang X. et al, 2018 ). In addition, Wnt signaling, cell polarity and metabolic property also enrich the transduction mechanism of Hippo-YAP signaling pathway ( Totaro et al, 2018b ; Xiang et al, 2018 ; Moya and Halder, 2019 ).…”