LRH-1 high expression in the ovarian granulosa cells of PCOS patients

Abstract

Purpose ： Polycystic ovary syndrome (PCOS) is considered as one of the most common endocrine disorder with heterogeneity. There are also reports that liver receptor homolog 1 [LRH-1 or nuclear receptor subfamily 5 group A member 2 (NR5A2)] plays an important role in the reproductive system. But up to now, there are no reports related to the link with PCOS and LRH-1. In this study, we aimed to detect the LRH-1 expression in the ovarian granulosa cell of PCOS patients and explore the potential relationship between LRH-1 and PCOS. Methods：146 follicular fluid sample were collected in this study, including 72 from PCOS patients and 74 from control patients who underwent intracytoplasmic sperm injection (ICSI) or in vitro fertilization-embryo transfer (IVF-ET). The ovarian granulosa cells were extracted from the patient's follicular fluid by magnetic-activated cell sorting (MACS) method, and the real-time quantitative PCR (qRT-PCR) was used to measure the expression of LRH-1 in ovarian granulosa cells. Then we analyzed the correlation between the expression level of LRH-1 and the clinical characteristics of patient by using Pearson Correlation analysis. Results：The expression of LRH-1 was significantly higher in PCOS patients ovarian granulosa cells than that in the control patients [vs(1.38±0.47)vs(1.03±0.32), t=5.327, p<0.0001], and it was positively correlated with antral follicles counting (AFC) (r=0.3607, p<0.0001)and the serum AMH(r=0.2662, p=0.0012)\LH(r=0.2518, p=0.0022)\T(r=0.2516, p=0.0022) in all patients. No statistical significance between LRH-1 and BMI, FSH, HOMA-IR, DHE-S, progesterone.Conclusions ： Compared with the control group, we found that LRH-1 was highly expressed in the ovarian granulosa cells of PCOS patients. Our study has revealed the relationship between the LRH-1 expression and PCOS, which suggested that LRH-1 may play an important role in ovulation disorders.While this finding provided new ideas for the study of the pathogenesis, it also provided a theoretical basis for the clinical diagnosis and treatment for PCOS.

Is Higher Docetaxel Clearance in Prostate Cancer Patients Explained by Higher CYP3A? An In Vivo Phenotyping Study with Midazolam

“…34 Finally, several studies have investigated the relationship between androgens, such as testosterone, and Lrh-1; however, the exact relationship remains to be elucidated. [35][36][37][38][39] In the current study, no correlation was observed between testosterone levels and midazolam CL. Moreover, a comparison in oral docetaxel pharmacokinetics between patients with mCRPC and newly diagnosed hormonesensitive prostate cancer found no differences between the 2 groups.…”

“…Accordingly, Lrh‐1 knockout mice demonstrated increased docetaxel C max and AUC in plasma with lowered hepatic docetaxel concentrations 34 . Finally, several studies have investigated the relationship between androgens, such as testosterone, and Lrh‐1; however, the exact relationship remains to be elucidated 35‐39 . In the current study, no correlation was observed between testosterone levels and midazolam CL.…”