LRRC75A antisense lncRNA1 knockout attenuates inflammatory responses of bovine mammary epithelial cells

Jing

Oxidative Medicine and Cellular Longevity

et al. 2021

Mastitis is a common disease in the dairy industry that causes huge economic losses worldwide. Exosomes (carrying proteins, miRNA, lncRNA, etc.) play a vital role in the regulation of immune response. lncRNA can play a variety of regulatory roles by combining with protein, RNA, and DNA. The expression of mRNA and lncRNA in exosomes derived from bovine mammary epithelial cells infected by S. aureus is rarely understood. To explore this issue, RNA sequencing analysis was performed on exosomes derived from S. aureus-infected and noninfected MAC-T cells. Analysis of the sequencing results showed that there were 186 differentially expressed genes, 431 differentially expressed mRNAs and 19 differentially expressed lncRNAs in the exosomes derived from S. aureus-infected and noninfected MAC-T cells. By predicting lncRNA target genes, it was found that 19 differentially expressed lncRNAs all acted on multiple mRNAs in cis and trans. GO analysis revealed that differentially expressed genes and lncRNA target genes played significant roles in such metabolism (reactive oxygen species metabolic processes), transmembrane transport, cellular response to DNA damage stimulus, and response to cytokines. KEGG enrichment indicated that lncRNA target genes gathered in the TNF pathway, Notch pathway, MAPK pathway, NF-kappa B pathway, Hippo pathway, p53 pathway, reactive oxygen species metabolic processes, and longevity regulating pathway. In summary, all data indicated that differentially expressed gene, mRNA, and lncRNA in transcriptional profiling of exosomes participated in bacterial invasion and adhesion, oxidative stress, inflammation, and apoptosis-related signaling pathway. The data obtained in this study would provide valuable resource for understanding the lncRNA information in exosomes derived from dairy cow mammary epithelial cells and conduced to the study of S. aureus infection in dairy cow mammary glands.

Section: Introductionmentioning

confidence: 99%

Transcriptional Profiling of Exosomes Derived from Staphylococcus aureus‐Infected Bovine Mammary Epithelial Cell Line MAC‐T by RNA‐Seq Analysis

Jing

Oxidative Medicine and Cellular Longevity

et al. 2021

“…They can interact with coding genes in both cis and trans manners to fulfill their functions [ 40 ]. It was found that a new lncRNA, LRRC75A-AS1, could protect its maternal gene ( LRRC75A ) from being degraded and promote inflammation [ 41 ]. Therefore, we performed KEGG and GO analyses to predict the functions of lncRNAs based on the functions of the maternal genes.…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Analysis of LncRNA in Bovine Mammary Epithelial Cell Injuries Induced by Escherichia Coli and Staphylococcus Aureus

Lin

Zhu

Hao

et al. 2021

IJMS

Escherichia coli and Staphylococcus aureus are two common pathogenic microorganisms that cause mastitis in dairy cows. They can cause clinical mastitis and subclinical mastitis. In recent studies, lncRNAs have been found to play an important role in the immune responses triggered by microbial inducers. However, the actions of lncRNAs in bovine mastitis remain unclear. The purpose of this study was to investigate the effects of bovine mammary epithelial cell injuries induced by treatment with E. coli and S. aureus, and to explore the lncRNA profile on cell injuries. The lncRNA transcriptome analysis showed a total of 2597 lncRNAs. There were 2234 lncRNAs differentially expressed in the E. coli group and 2334 in the S. aureus group. Moreover, we found that the E. coli and S. aureus groups of maternal genes targeted signaling pathways with similar functions according to KEGG and GO analyses. Two lncRNA–miRNA–mRNA interaction networks were constructed in order to predict the potential molecular mechanisms of regulation in the cell injuries. We believe that this is the first report demonstrating the dysregulation of lncRNAs in cells upon E. coli and S. aureus infections, suggesting that they have the potential to become important diagnostic markers and to provide novel insights into controlling and preventing mastitis.

“…Emerging reports has revealed that LRRC75A-AS1 was involved in several biological processes through modulation of signaling pathway, Jeong et al [13] reported that LRRC75A-AS1 can regulate the vascular calcification negatively, and might act as a possible target in the treatment of vascular calcification. Wang et al [14] found that LRRC75A-AS1 can regulate the expression of tight junction (TJ) proteins through LRRC75A, affecting the inflammatory responses of bovine mammary epithelial cells. Leavey K et al [15] show that LRRC75A is abnormally expressed in the process of normal villous maturation.…”

Section: Discussionmentioning

confidence: 99%

The Association Between lncRNA LRRC75A-AS1 and The Clinical Characteristics in Neuroblastoma

Wang¹,

Wang

et al. 2020

BJSTR

Background: Neuroblastoma is the highest mortality rate extracranial soild tumor in childhood. Accumulating evidence indicated that long noncoding RNAs (lncRNAs) are widely expressed in neuroblastoma, and playing an important role in the development and progression.Methods: RNA sequencing was conducted to identify differentially expressed ln-cRNAs in four Ⅲ phase and four Ⅳ phase tumor tissues of neuroblastoma. RT-qPCR was carried out to validate the result of sequencing. Clinical information was reviewed to analyze the relationship between lncRNA and clinical characteristics. The public database R2 was used to analyze prognosis.Result: Differentially expressed lncRNAs were identified. LRRC75A-AS1 was the overexpressed lncRNA in Ⅳ phase patients. RT-qPCR was conducted in tumor tissues, confirming the tendency with sequencing. And higher expression of LRRC75A-AS1 was associated with N-MYC (p < 0.001), advanced stage (p = 0.029), Risk group (p = 0.027). Furthermore, LRRC75A-AS1 was correlated with Shimada classification(p = 0.046), LDH level (r = 0.390, p = 0.003), D-Dimer level (r = 0.338, p = 0.012) , and NSE level (r = 0.284, p = 0.05). The neuroblastoma dataset shows that patients with overexpressed LRRC75A-AS1 have a worse prognosis than down-expressed. Conclusion:LRRC75A-AS1 is associated with clinical characteristics of neuroblastoma and may function as a prognostic predictor or a therapeutic target.