LSPR immuno-sensing based on iso-Y nanopillars for highly sensitive and specific imidacloprid detection

Vestri, Ambra; Rippa, Massimo; Marchesano, Valentina; Sagnelli, Domenico; Margheri, Giancarlo; Zhou, Jun; Petti, Lucia

doi:10.1039/d1tb01344k

Cited by 19 publications

(11 citation statements)

References 53 publications

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“…As previously described, 18 the spectral shifts of the LSPR peaks were measured using an experimental setup consisting of a halogen lamp that provides unpolarized white light to the nanostructured pattern by the use of a 10X (N.A. 0.25) objective (a circular light spot of about 400 μm).…”

Section: Methodsmentioning

confidence: 99%

“…The plasmonic properties of nanoscale systems and their sensing performance are jointly determined by physicochemical factors such as dielectric properties and the material composition as much as geometric factors. For this reason, the shape, size, period, and symmetry of the engineered plasmonic nanostructure are designed to obtain enhanced field distribution at the desired wavelength or even the effective sensing volume available for a particular analyte. − Over the years, our team had also empirically observed that some patterns of specific pitches provide superior performance for a particular analyte, and so we have developed LSPR- and SERS-based sensors of molecules, biomacromolecules, and viruses, e.g., Thiram pesticide on Thue-Morse arranged triangular nanopillars, bovine serum albumin and imidacloprid insecticide on iso-Y-shaped nanopillars, , rotavirus on Achiral Octupolar arranged triangular nanopillars, and hepatitis A on pyramidal nanoholes . In this study, we explore the functionality and limitations of AAL on a 2D plasmonic photonic crystal–glass/ITO substrate with gold triangular pillars arranged in Achiral Octupolar pattern with a pitch of 25, 50, 100, and 263 nm (AchOct 25/50/100/263).…”

Section: Introductionmentioning

confidence: 99%

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DNA Antiadhesive Layer for Reusable Plasmonic Sensors: Nanostructure Pitch Effect

et al. 2022

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A long-term reusable sensor that provides the opportunity to easily regenerate the active surface and minimize the occurrence of undesired absorption events is an appealing solution that helps to cut down the costs and improve the device performances. Impressive advances have been made in the past years concerning the development of novel cutting-edge sensors, but the reusability can currently represent a challenge. Direct shielding of the sensor surface is not always applicable, because it can impact the device performance. This study reports an antiadhesive layer (AAL) made of 90 mg/mL DNA sodium salt from salmon testes (ssstDNA) for passivating gold plasmonic sensor surfaces. Our gold two-dimensional (2D) nanostructured plasmonic metasurfaces modified with AAL were used for DNA quantification. AAL is thin enough that the plasmonic sensor remains sensitive to subsequent deposition of DNA, which serves as an analyte. AAL protects the gold surface from unwanted nonspecific adsorption by enabling wash-off of the deposited analyte after analysis and thus recovery of the LSPR peak position (rLSPR). The calibration curve obtained on a single nanostructure (Achiral Octupolar, 100 nm pitch) gave an LOD = 105 ng/mL and an extraordinary dynamic range, performances comparable or superior to those of commercial UV–vis spectrometers for acid nucleic dosage. Two different analytes were tested: ssstDNA (∼2000 bp) in deionized water and double-strand DNA (dsDNA) of 546–1614 bp in 100 mM Tris buffer and 10 mM MgCl 2 . The two nanostructures (Achiral Octupolar 25 and 100) were found to have the same sensitivity to DNA in deionized water but different sensitivity to DNA in a salt/buffer solution, opening a potential for solute discrimination. To the best of our knowledge, this is the first report on the use of AAL made of several kilobase-pairs-long dsDNA to produce a reusable plasmonic sensor. The working principle and limitations are drawn based on the LSPR and SERS study.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

DNA Antiadhesive Layer for Reusable Plasmonic Sensors: Nanostructure Pitch Effect

et al. 2022

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“…The array was fabricated by EBL lithography, its optical response was numerically evaluated, and a sensitivity of 412 nm/RIU was measured. The proposed platform was functionalized with IgG and allowed for the detection of a pesticide (namely, the imidacloprid) in the dynamic range of 1-1000 ng/mL achieving an LOD of 1 ng/mL [106]. Chen et al proposed instead a multiplexed detection based on IgGs using large-scale plasmonic nanoarrays based on thermal dewetting of gold films of 10 nm thickness.…”

Section: Lspr-based Plasmonic Nanoarraysmentioning

confidence: 99%

Plasmonic Nanosensors: Design, Fabrication, and Applications in Biomedicine

et al. 2022

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Current advances in the fabrication of smart nanomaterials and nanostructured surfaces find wide usage in the biomedical field. In this context, nanosensors based on localized surface plasmon resonance exhibit unprecedented optical features that can be exploited to reduce the costs, analytic times, and need for expensive lab equipment. Moreover, they are promising for the design of nanoplatforms with multiple functionalities (e.g., multiplexed detection) with large integration within microelectronics and microfluidics. In this review, we summarize the most recent design strategies, fabrication approaches, and bio-applications of plasmonic nanoparticles (NPs) arranged in colloids, nanoarrays, and nanocomposites. After a brief introduction on the physical principles behind plasmonic nanostructures both as inherent optical detection and as nanoantennas for external signal amplification, we classify the proposed examples in colloid-based devices when plasmonic NPs operate in solution, nanoarrays when they are assembled or fabricated on rigid substrates, and nanocomposites when they are assembled within flexible/polymeric substrates. We highlight the main biomedical applications of the proposed devices and offer a general overview of the main strengths and limitations of the currently available plasmonic nanodevices.

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“…The protein layers can be identified from observing the difference in the diffraction pattern of the array before and after functionalization with the protein layer, which is a label-free technique that provides miniaturization and easier integration. With appropriate functionalization, gold nanoparticles can even provide high specificity to particular analytes [ 97 ].…”

Section: Plasmonic Materialsmentioning

confidence: 99%

“… Versatility of gold nanoparticles; (from left to right) nanospheres [ 60 ], nanostars [ 88 ], nanorods [ 89 ], nanocages [ 92 ], nanodiscs [ 90 ], nanoholes [ 93 ], and iso-Y [ 97 ], to support plasmonic resonance all over the visible range. Nanostars and nanodiscs can support multiple resonances.…”

Section: Figurementioning

confidence: 99%

Materials Perspectives of Integrated Plasmonic Biosensors

et al. 2022

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With the growing need for portable, compact, low-cost, and efficient biosensors, plasmonic materials hold the promise to meet this need owing to their label-free sensitivity and deep light–matter interaction that can go beyond the diffraction limit of light. In this review, we shed light on the main physical aspects of plasmonic interactions, highlight mainstream and future plasmonic materials including their merits and shortcomings, describe the backbone substrates for building plasmonic biosensors, and conclude with a brief discussion of the factors affecting plasmonic biosensing mechanisms. To do so, we first observe that 2D materials such as graphene and transition metal dichalcogenides play a major role in enhancing the sensitivity of nanoparticle-based plasmonic biosensors. Then, we identify that titanium nitride is a promising candidate for integrated applications with performance comparable to that of gold. Our study highlights the emerging role of polymer substrates in the design of future wearable and point-of-care devices. Finally, we summarize some technical and economic challenges that should be addressed for the mass adoption of plasmonic biosensors. We believe this review will be a guide in advancing the implementation of plasmonics-based integrated biosensors.

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LSPR immuno-sensing based on iso-Y nanopillars for highly sensitive and specific imidacloprid detection

Abstract: A label-free sensitive and specific LSPR immunosensor for imidacloprid detection based on a 2D nanostructured metasurface.

Cited by 19 publications

References 53 publications

DNA Antiadhesive Layer for Reusable Plasmonic Sensors: Nanostructure Pitch Effect

DNA Antiadhesive Layer for Reusable Plasmonic Sensors: Nanostructure Pitch Effect

Plasmonic Nanosensors: Design, Fabrication, and Applications in Biomedicine

Materials Perspectives of Integrated Plasmonic Biosensors

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