&lt;b&gt;Evaluation of quantitative parameters of Leydig cell in diabetic adults rats

Silva, Alluanan Adelson do Nascimento; Oliveira, Jessica Santana de; Oliveira, Rodrigo Ribeiro de; Moraes, Sílvia Regina Arruda de; Júnior, Valdemiro Amaro da Silva; Melo, Elizabeth Neves de

doi:10.4025/actascibiolsci.v36i4.22455

Cited by 7 publications

(5 citation statements)

References 21 publications

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“…for 60 days. GSI is inversely proportional to the reproductive efficiency of the rats[ 57 ] and testicular coefficient (TCT) value, which is directly proportional to reproductive toxicity in testes. [ 58 ] A marginal increase in the GSI in group E4D and E7D was observed after the treatment with piperine for 60 days.…”

Section: Resultsmentioning

confidence: 99%

Reversible testicular toxicity of piperine on male albino rats

Chinta¹,

Coumar²,

Periyasamy³

2017

Phcog Mag

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Background:Piperine was widely used in traditional medicine for inducing sterility and abortion.Objective:To evaluate the effect of the piperine on testis of male albino ratsMaterials and Methods:Adult male rats were divided into four groups (n = 12). Group I (control): Rats were given vehicle p.o. i.e. 0.5% carboxymethyl cellulose in normal saline daily for 60 days, Group II (ED): Rats received piperine at a dose of 10 mg/kg body weight (b.w.) daily, Group III (E4D): Rats received piperine at a dose of 10 mg/kg b.w. on every 4th day, Group IV (E7D): Rats received piperine at a dose of 10 mg/kg b.w. on every 7th day. Half of the animals from each group were sacrificed after the treatment period (60 days), and the remaining were kept for drug-free withdrawal period (60 days) and then sacrificed.Results:Piperine significantly decreased the reproductive organ weights in groups ED and E4D. Piperine induced hormonal imbalance by altering the serum levels of follicle-stimulating hormone, luteinizing hormone, sex hormone binding globulin, serum, and testicular testosterone in groups ED and E4D. Furthermore, piperine decreased the activity of germ cell markers and Leydig cellular steroidogenic enzymes in the groups ED and E4D after 60 days. All the above-altered values returned to normal levels after withdrawal period. Histopathological findings also supported the above findings.Conclusion:From the above data, it can be concluded that piperine could be a good lead molecule for the development of reversible oral male contraceptive.SUMMARY Piperine was employed for the contraceptive purposes in traditional medicinePiperine significantly impaired the spermatogenesis by decreasing the testicular hormone synthesis in groups ED and E4DPiperine disrupted the testicular antioxidant system by promoting the ROS production and hydroxyl radical generation in rat testis in groups ED and E4DHistopathological evidence supported the disruption of spermatogenesis by piperineAll the effects of piperine after the treatment period (i.e. 60 days) were back to normal after the withdrawal period (i.e., after 120 days).

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Section: Resultsmentioning

confidence: 99%

Reversible testicular toxicity of piperine on male albino rats

Chinta¹,

Coumar²,

Periyasamy³

2017

Phcog Mag

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“…A reduced testicular weight and GSI (a better way to assess the damage to the testes in relation to the body) in experimental rats is most likely due to decreased levels of male sex hormones and androgen especially plays a pivotal role in sex organs [31]. GSI is inversely proportional to the reproductive efficiency of the rats [32] and a pertinent value to be accessed in reproductive studies. When compared to the treated groups, the GSI was higher in the control group, suggesting a reduction in the animals' commitment to spermatic production after receiving the concentrated extract.…”

Section: Discussionmentioning

confidence: 99%

Effects of aqueous methanolic extract of Bridella ferruginea stem bark on the spermatogenesis, accessory reproductive organs, and organosomatic indices in pubertal male Rattus Norvegicus

Engel¹,

Ahur²,

Agu³

et al. 2023

GSC Biol. Pharm. Sci.

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There is an increasing trend in the usage of medicinal plants in livestock production with some significantly toxic effects especially to vital organs such as reproductive tissues which necessitates this search. Twenty (20) matured male rats weighing 150-239g randomly assigned into 4 groups (n=5) were used for the sub-acute toxicity. Group 1 rats were treated with distilled water while groups 2-4 were treated with 100, 200, and 400mg/kg respectively of the aqueous methanolic extract of Bridelia ferruginea stem bark (AMEBFSB) for 30 days. Animals were terminated on day 31 and some of the reproductive organs were collected and weighed. The Cauda epididymis served as a semen source for sperm analysis and testes were collected for histological examination. There was a significant decrease (p<0.05) in the final body weights, average weights of testes, epididymis, vas deferens, seminal vesicles, and prostate gland of the treated groups as compared to the control. The semen evaluated showed a significant decrease (p<0.05) in sperm motility, sperm count, and percent normal sperm cell morphology in the 400mg/kg group, no significant difference (p<0.05) in the sperm viability and acrosome integrity as compared to control. Photomicrograph revealed loss and thinning of germinal epithelium and widened seminiferous tubule lumen. In conclusion, the oral administration of AMEBFSB for 30 days in male rats showed that the plant has antispermatogenic effects on reproductive functions.

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“…Cytoplasmic and nuclear volumes were then calculated (cytoplasmic volume = % cytoplasm × nuclear volume/% nucleus; cellular volume = nuclear volume + cytoplasmic volume). The Leydig cell population per testis and gram of testis were calculated by using the volume of one Leydig cell and the total volume of Leydig cells in the testis 12,47 …”

Section: Methodsmentioning

confidence: 99%

“…Besides, in the same animals, Leydig cells had decreased cytoplasmic volume, cellular volume and population. Several studies show alterations in number, 73,74,78,82 volume 47 and ultrastructure 79,83 of Leydig cells in the offspring of diabetic females and adult diabetic According to Kianifard et al, 83 reductions in the cytoplasmic and cellular volume of Leydig cells may indicate an involution of the smooth endoplasmic reticulum (sER), which is the most abundant organelle in this cell. Besides, since the sER is responsible for Leydig cell function, its involution may reflect altered steroid hormone production.…”

Section: Componentsmentioning

confidence: 99%

Histomorphometric and oxidative evaluation of the offspring's testis from type 2 diabetic female rats treated with metformin and pentoxifylline

Oliveira

Silva

Dias

et al. 2022

Int J Experimental Path

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Type 2 diabetes mellitus (T2D) during pregnancy is characterized by high levels of reactive oxygen species and pro-inflammatory factors in the placenta. Once these reactive species reach the foetus, they trigger physiological adaptations that allow the foetus to survive, but programme the organism to develop metabolic disorders in adulthood. The male reproductive system is highly susceptible to foetal programming. This study aimed to investigate the effects of intrauterine exposure to T2D on testicular histomorphometry and redox homeostasis of adult rats and evaluate the effects of maternal treatment with metformin and pentoxifylline. Female rats were induced to T2D, then treated with metformin and pentoxifylline, or co-treated with both drugs. The females were mated, the male offspring were sacrificed on postnatal day 90, and the testicles were collected for analysis. Metformin protected the tubular compartment, with the maintenance of the Sertoli cell population and daily sperm production. Pentoxifylline attenuated the effects of diabetes on Leydig cells, in addition to stimulating testosterone production and lowering lipid peroxidation. Intrauterine exposure to T2D results in important testicular alterations that compromise gonadal function, and the cotreatment with metformin and pentoxifylline may represent a promising therapy that attenuates these effects by combining the positive influences in both the tubular and interstitial compartments of the testicular parenchyma.

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<b>Evaluation of quantitative parameters of Leydig cell in diabetic adults rats

Cited by 7 publications

References 21 publications

Reversible testicular toxicity of piperine on male albino rats

Reversible testicular toxicity of piperine on male albino rats

Effects of aqueous methanolic extract of Bridella ferruginea stem bark on the spermatogenesis, accessory reproductive organs, and organosomatic indices in pubertal male Rattus Norvegicus

Histomorphometric and oxidative evaluation of the offspring's testis from type 2 diabetic female rats treated with metformin and pentoxifylline

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