<i>Astraglaus polysaccharide</i> protects diabetic cardiomyopathy by activating NRG1/ErbB pathway

Abstract

Diabetic cardiomyopathy (DCM) is one of the main cardiac complications among diabetic patients. According to previous studies, the pathogenesis of DCM is associated with oxidative stress, apoptosis and proliferation of local cardiac cells. It showed, NRG1 can improve the function of mitochondria, and thereby, increasing proliferation and decreasing apoptosis of cardiac muscle cell via ErbB/AKT signaling, also, exert antioxidative function. Besides, NRG1/ErbB pathway was impaired in the DCM model which suggested this signaling played key role in DCM. Astraglaus polysaccharide (APS), one of the active components of Astragalus mongholicus, showed striking antioxidative effect. Here, in this study, our data showed that APS can promote proliferation and decrease apoptosis in AGE-induced DCM cell model, besides, APS can decrease intracellular ROS level, increase activity of SOD, GSH-Px and lower level of MDA and NO in DCM cell model, indicating APS exerted antioxidative function in DCM model cells. Besides, western blot results revealed APS induced NRG1 expressing and the phosphorylation level of ErbB2/4. In addition, the elevated NRG1 promoted AKT and PI3k phosphorylation which indicated APS may exert its function by NRG1/ErbB and the downstream AKT/PI3K signaling. Canertinib is ErbB inhibitor. The effect of APS on proliferation, apoptosis, antioxidation and NRG1/ErbB pathway was partly abolished after the cells were co-treated with APS and canertinib. Taken together, these results suggested APS may display its protective function in DCM cells by activating NGR1/ErbB signaling pathway. And our study increased potential for prevention and therapy to DCM.

Coriolus versicolor alleviates diabetic cardiomyopathy by inhibiting cardiac fibrosis and NLRP3 inflammasome activation

“…In addition, our previous studies revealed that polysaccharides were the main active compounds of CV aqueous extract. Numerous studies have reported that polysaccharides protect against DCM by inhibiting oxidative stress or apoptosis or by activating heart chymase or the NRG1/ErbB pathway (Chang, Lu, Wang, Lv, & Fu, ; Chen et al, ; Sun et al, ). These findings suggest that CV extract has a functional role in protecting heart function in DCM.…”

Shenqi Fuzheng Injection impairs bile duct ligation-induced cholestatic liver injury in vivo

“…In addition, Chu et al have reported that Codonopsis displayed an anti-oxidative effect by decreasing MDA levels and increasing GSH-PX in a mouse model of chronic obstructive pulmonary disease [16]. Chang et al also showed that Astragalus decreased intracellular ROS levels, increased the activity of SOD, and lowered the levels of MDA [17]. Therefore, since SFI was prepared from Codonopsis and Astragalus , prior studies suggested that the observed therapeutic effects of SFI might be related to the anti-inflammatory and anti-oxidative activities of both of those traditional Chinese medicinal herbs.…”

A Systematic Review of Phytochemistry, Pharmacology and Pharmacokinetics on Astragali Radix: Implications for Astragali Radix as a Personalized Medicine

“…Moreover, NRG1/ErbB can improve glucose tolerance in healthy and diabetic rodents [103] and regulate the oxidative capacity of myocyte [104,105]. It was confirmed that APS could increase proliferation, inhibit apoptosis and improve antioxidative function including reducing intracellular ROS level, elevating activity of GSH-Px and SOD and lowering the level of MDA and NO by activating the NRG1/ErbB pathway [106] (Figure 3⑧). What is more, substantial reports suggested that the diabetic heart is characterized by reduced glucose metabolism and enhanced fatty acid utilization [107].…”