<p>Curcumin-loaded nanostructured lipid carriers prepared using Peceol™ and olive oil in photodynamic therapy: development and application in breast cancer cell line</p>

Abstract

Purpose To potentiate the anticancer activity of curcumin (CUR) by improving its cell penetration potentials through formulating it into nanostructured lipid carriers (NLCs) and using the prepared NLCs in photodynamic therapy. Methods A 3×4 factorial design was used to obtain 12 CUR-NLCs using two factors on different levels: (1) the solid lipid type at four levels and (2) the solid to liquid lipid ratio at three levels. Olive oil, Tween 80 and lecithin were chosen as liquid lipid, surfactant and co-surfactant, respectively. CUR-NLCs prepared by high shear hot homogenization method were evaluated by determination of particle size (PS), polydispersity index, zeta potential (ZP), entrapment efficiency percent, drug loading percent and in vitro drug release. Optimization was based on the evaluation results using response surface modeling (RSM). Optimized formulae were tested for their in vitro release pattern and for dark and photo-cytotoxic anticancer activity on breast cancer cell line in comparison to free CUR. Results Evaluation tests showed the appropriateness of NLCs prepared from glyceryl monooleate and Geleol™ helped choosing two optimized formulae, PE3 and GE3. PE3 (prepared using glyceryl monooleate) showed enhanced release rates compared to GE3 (prepared from Geleol) and superior cytotoxic anticancer activity compared to both GE3 and free CUR under both light and dark conditions. The small mean PS, spherical shape as well as the negative ZP enhanced the internalization of the NLCs within cells. Modulation and inhibition of P-glycoprotein expression by glyceryl monooleate synergized the cytotoxic activity of CUR. Conclusion CUR loading in NLCs enhanced its cell penetration and cytotoxic anticancer properties both in dark and in light conditions.

<p>^99mTc Radiolabeled HA/TPGS-Based Curcumin-Loaded Nanoparticle for Breast Cancer Synergistic Theranostics: Design, in vitro and in vivo Evaluation</p>

“…[2][3][4][5][6][7][8][9] In the past decades, nanocarriers have been rapidly developed for drug delivery to increase solubility, prolong duration in vivo, improve tumor-selective cytotoxicity, and reduce drug exposure to normal tissues including polymeric nanoparticles, inorganic nanoparticles, and liposomes. [10][11][12][13][14][15][16][17][18] Polymeric nanocarriers may be composed of a synthetic polymer or natural polymer, both of which have exhibited good biocompatibility, an easily manipulated chemical structure, and stimuli-responsiveness. [19][20][21][22] Hyaluronic acid, also called hyaluronan (HA), a natural polysaccharide, consists of repeating disaccharide units and has been widely used for its biocompatibility.…”

Innovative Delivery Systems Loaded with Plant Bioactive Ingredients: Formulation Approaches and Applications

“…Increased anticancer activity was noticed under both dark and light conditions [ 155 ]. In vitro digestion and release studies were performed for curcumin-loaded NLCs that were found to exhibit controlled release while the system was found to be stable under the simulated digestion conditions for 2 h [ 156 ]. For the increase in the (low) antiplasmodial activity of free curcumin due to its low bioavailability, NLCs were developed and the results were found to be promising for the treatment of malaria [ 157 ].…”

New Peceol™/Span™ 60 Niosomes Coated with Chitosan for Candesartan Cilexetil: Perspective Increase in Absolute Bioavailability in Rats

“…GMO is a lyotropic liquid which can form different crystalline phases in water (cubic, hexagonal, and lamellar), depending on its critical packing parameter (CPP) alone or in combination with other materials. 5,6 It possessed a beneficial role in improving the drug absorption via lowering P-gp activity. 6 Peceol™ also played a vital role in drug lymphatic transport.…”