<p>High Frequency Pulsatile Electromagnetic Fields and Ultrasound Pulsatile Fields Impact on Germination Dynamic at <i>Ocimum basilicum</i> L. and <i>O. basilicum</i> var. <i>purpurascens</i> Benth., Observed with Open Source Software’s</p>

Abstract

Basil (Ocimum sp.) is generally known as "king of herbs" due to its pharmaceutical and culinary properties. Even though Ocimum sp. are considered to have fast germination velocity index (GVI), short mean germination time (MGT) and increased seedling vigor index (SVI) values, their experimental monitoring is usually done with specialized software's that are expensive. Low cost scientific solutions are keen on open source software germination protocols. For observing the accuracy of open source, C.A.D. softwares were used for Ocimum sp. seeds which were submitted to high frequency pulsatile electromagnetic fields (300 impulses/3 peak penetrance -293 W) and ultrasound pulsatile fields (1/2 pulses by 0.5 W/cm 2 ). All sequence images where taken using a positive/negative photo filter enhancing the differences in seed development. At the end of the experiment, all images where transformed into vector formats (dwg). On the dwg extension, selective free plug-ins such as Face Centroid and Area Properties helped to collect data like seed development on X/Y scale, area, perimeter, no. of germinated seeds, length of seedling root, hypocotyl length. Mean germination time (MGT) was in agreement with the accepted theory of seed germination that enounced a diametric position between values of GVI and MGT, meaning that all the experimental variants with high values of GVI recorded low values of MGT. All the experimental variants influenced by physical stimulants recorded improved contents of therapeutical terpenes.

Total and Potentially Active Bacterial Communities Entrapped in a Late Glacial Through Holocene Ice Core From Scarisoara Ice Cave, Romania

“…Scarisoara Ice Cave (Romania), the most extensively studied ice cave (Perşoiu et al, 2017), hosts the world’s oldest and largest perennial ice block (Holmlund et al, 2005). Over the last century, study of this underground ice block disentangled the climatic and glaciological associated processes in this cave (Racovita and Onac, 2000; Perşoiu et al, 2011b) and resulted in the successful reconstruction of climatic and environmental changes in the region (Onac et al, 2007; Feurdean et al, 2011; Perşoiu and Pazdur, 2011; Perşoiu et al, 2017). An early report revealed the presence of nitrifying bacteria in limestone sediments from this cave (Pop, 1949).…”