<p>Long Non-Coding RNA NEAT1 Serves as Sponge for miR-365a-3p to Promote Gastric Cancer Progression via Regulating ABCC4</p>

Abstract

Long non-coding RNA (lncRNA) was reported to be a crucial regulator in cancer. In this work, our purpose is to explore the biological roles of nuclear paraspeckle assembly transcript 1 (NEAT1) in gastric cancer (GC). Methods: Quantitative real-time polymerase chain reaction (qRT-PCR) was performed to detect NEAT1 expression in GC cells and normal cells. GC cell behaviors after NEAT1 overexpression or downregulation were analyzed by Cell Counting Kit-8 assay, colony formation assay, woundhealing assay, and flow cytometry assay. Bioinformatic tools were used to analyze the significance of NEAT1 in GC. The involvement of microRNA-365a-3p (miR-365a-3p) and ATP-binding cassette subfamily C member 4 (ABCC4) in the biological roles of NEAT1 in GC progression was validated by luciferase activity reporter assay and rescue experiments. Results: We found NEAT1 increased expression in both GC tissues and cells and correlated with poorer overall survival of cancer patients. We found NEAT1 overexpression promotes, while its knockdown inhibits GC cell proliferation, colony formation, invasion, and cell cycle progression in vitro. Mechanism analyses showed that NEAT1 serves as a ceRNA to upregulate ABCC4 expression via sponging miR-365a-3p. Conclusion: In this study, we revealed a NEAT1/miR-365a-3p/ABCC4 triplet in GC progression, which may provide novel targeted therapy markers for GC.

Interplay between endoplasmic reticulum stress and non-coding RNAs in cancer

“…In addition, lncRNA nuclear-enriched abundant transcript 1 (NEAT1) is newly discovered and has been localized in cell nuclear paraspeckles. Increasing evidence has revealed that NEAT1 is upregulated in multiple cancers and facilitates cell invasion and migration by being a sponge for miRNAs [137][138][139][140]. Recently, NEAT1 has been proven to be highly expressed in multiple myeloma, and knockdown of NEAT1 inhibited the invasion and metastasis of myeloma cells.…”

LncRNA Nuclear-Enriched Abundant Transcript 1 Regulates Atrial Fibrosis via the miR-320/NPAS2 Axis in Atrial Fibrillation

“…Several existing studies have indicated that lncRNAs participate in the ceRNAs regulatory network to negatively regulate the miRNAs expression ( Yan et al, 2018 ), such as NEAT1. For instance, NEAT1 functions as a sponge for miR-365a-3p to facilitate gastric cancer progression through targeting ABCC4 ( Gao et al, 2020 ); furthermore, NEAT1 sponges miR-129 to regulate renal fibrosis via modulating coll I ( Li et al, 2020 ). To determine the mechanism of NEAT1 regulation in AF, we performed bioinformatics databases and discovered that NEAT1 shared miR-320 response element with NPAS2.…”

LncRNA NEAT1 promotes nucleus pulposus cell matrix degradation through regulating Nrf2/ARE axis

“…LncRNA NEAT1 plays an important role in many diseases, such as gastric cancer [ 24 ], prostate cancer [ 25 ], renal fibrosis [ 26 ], and osteosarcoma [ 27 ]. What is more, Liu et al [ 28 ] found that knockdown of lncRNA NEAT1 inhibit SK-N-SH cells apoptosis, inflammation and cytotoxicity.…”