<p>Long noncoding RNA DANCR contributes to docetaxel resistance in prostate cancer through targeting the miR-34a-5p/JAG1 pathway</p>

Abstract

Background: Chemotherapy is one of the available options for prostate cancer (PC). However, the acquisition of chemoresistance has become a major cause of chemotherapy failure. The long noncoding RNA DANCR is demonstrated to serve as an oncogene in various human cancers, including PC. However, the potential role of DANCR in docetaxel (DTX) resistance of PC and its underlying mechanism remains unclear. Methods: The abundance of DANCR, miR-34a-5p, and JAG1 mRNA was examined by quantitative reverse transcription PCR. The Cell Counting Kit-8 (CCK8) was used to determine the 50% inhibitory concentration value. Cell viability was evaluated by CCK8 and colony-formation assays. Transwells were utilized to analyze cell migration and invasion ability. The protein levels of LRP, P-gp, MRP1, and JAG1 were measured by Western blot assay. The target relationship between DANCR and miR-34a-5p, as well as miR-34a-5p and JAG1, was demonstrated by dual-luciferase, RNA immunoprecipitation, and RNA pull-down analysis. Tumor xenograft was undertaken to confirm the effect of DANCR on DTX resistance in PC. Results: DANCR and JAG1 were significantly upregulated, but miR-34a-5p was downregulated in DTX-resistant PC. Silencing of DANCR improved the DTX efficacy in DTX-resistant PC cells. DANCR served as a competing endogenous RNA of miR-34a-5p, leading to the derepression of miR-34a-5p target JAG1, which eventually triggered the resistance to DTX in DTX-tolerated PC. Conclusion: The DANCR/miR-34a-5p axis enhanced DTX resistance of PC via targeting JAG1, providing a novel insight to improve chemotherapy for PC.

lncRNA 00312 Attenuates Cell Proliferation and Invasion and Promotes Apoptosis in Renal Cell Carcinoma via miR-34a-5p/ASS1 Axis

“…The interbinding function of lncRNA 00312 and miR-34a-5p was also confirmed by luciferase report assay. The miR-34a-5p has been proved to have important biological functions in many tumors, including RCC [23,26,27]. In addition, we further demonstrated that miR-34a-5p is involved in the inhibitory role of lncRNA 00312 on invasion and proliferation of RCC.…”

DARS-AS1 accelerates the proliferation of cervical cancer cells via miR-628-5p/JAG1 axis to activate Notch pathway

“…For instance, JAG1 is modulated by MALAT1/miR-125 signaling to promote tongue cancer development [ 39 ]. Besides, the discovery of DANCR/miR-34a-5p/JAG1 axis provides a novel perspective on the treatment of prostate cancer [ 40 ].…”

<p>Long Non-Coding Small Nucleolar RNA Host Genes (SNHGs) in Endocrine-Related Cancers</p>

“…150 In addition, stimulation of the DANCR/miR-34a-5p axis enhanced docetaxel-resistance in prostate cancer via targeting JAG1, which in turn activates the Notch signaling pathway. 13 Finally, SNHG14, 151 SNHG15, 152 and SNHG20 153 may all act as oncogenes in prostate cancer via targeting miR-613, miR-338-3p, and miR-6516-5p to promote cell proliferation, migration, and invasion.…”

“…12 Meanwhile, SNHG13 serves as a competing endogenous RNA (ceRNA) of miR-34a-5p, leading to the derepression of Jagged 1 (JAG1) expression, which eventually triggers resistance to docetaxel in prostate cancer. 13 This review aims to provide an overview on the current understanding of the regulation and function of SNHGs in endocrine-related cancers that arise from the endocrine glands or neuroendocrine tissues, including thyroid cancer, breast cancer, pancreatic cancer, ovarian cancer, and prostate cancer. 14…”