&lt;p&gt;&lt;em&gt;Hsa-MiR-590-3p&lt;/em&gt; Promotes the Malignancy Progression of Pancreatic Ductal Carcinoma by Inhibiting the Expression of p27 and PPP2R2A via G1/S Cell Cycle Pathway&lt;/p&gt;

Shi, Xiasheng; Sheng, Weiwei; Chen, Jia; Tang, Jingtong; Dong, Ming

doi:10.2147/ott.s260499

Cited by 9 publications

(5 citation statements)

References 31 publications

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“…The hsa-mir-590-3p is an important target in the TF-miRNA co-regulatory network. Previous studies have reported that the hsa-mir-590-3p may play a significant role in pancreatic cancer [ 54 ], colorectal cancer [ 55 ], prostate cancer [ 56 ], and also breast cancer [ 57 ] as a therapeutic biomarker. No study has yet reported that hsa-mir-590-3p may play any role in OC.…”

Section: Discussionmentioning

confidence: 99%

Exploring the Novel Computational Drug Target and Associated Key Pathways of Oral Cancer

Akhter

Kahtani

Sambawa

et al. 2022

CIMB

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Oral cancer (OC) is a serious health concern that has a high fatality rate. The oral cavity has seven kinds of OC, including the lip, tongue, and floor of the mouth, as well as the buccal, hard palate, alveolar, retromolar trigone, and soft palate. The goal of this study is to look into new biomarkers and important pathways that might be used as diagnostic biomarkers and therapeutic candidates in OC. The publicly available repository the Gene Expression Omnibus (GEO) was to the source for the collection of OC-related datasets. GSE74530, GSE23558, and GSE3524 microarray datasets were collected for analysis. Minimum cut-off criteria of |log fold-change (FC)| > 1 and adjusted p < 0.05 were applied to calculate the upregulated and downregulated differential expression genes (DEGs) from the three datasets. After that only common DEGs in all three datasets were collected to apply further analysis. Gene ontology (GO) and pathway analysis were implemented to explore the functional behaviors of DEGs. Then protein–protein interaction (PPI) networks were built to identify the most active genes, and a clustering algorithm was also implemented to identify complex parts of PPI. TF-miRNA networks were also constructed to study OC-associated DEGs in-depth. Finally, top gene performers from PPI networks were used to apply drug signature analysis. After applying filtration and cut-off criteria, 2508, 3377, and 670 DEGs were found for GSE74530, GSE23558, and GSE3524 respectively, and 166 common DEGs were found in every dataset. The GO annotation remarks that most of the DEGs were associated with the terms of type I interferon signaling pathway. The pathways of KEGG reported that the common DEGs are related to the cell cycle and influenza A. The PPI network holds 88 nodes and 492 edges, and CDC6 had the highest number of connections. Four clusters were identified from the PPI. Drug signatures doxorubicin and resveratrol showed high significance according to the hub genes. We anticipate that our bioinformatics research will aid in the definition of OC pathophysiology and the development of new therapies for OC.

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Section: Discussionmentioning

confidence: 99%

Exploring the Novel Computational Drug Target and Associated Key Pathways of Oral Cancer

Akhter

Kahtani

Sambawa

et al. 2022

CIMB

View full text Add to dashboard Cite

show abstract

“…The hsa-mir-590-3p is an important target in the TF-miRNA co-regulatory network. Previous studies have reported that the hsa-mir-590-3p may play a significant role in pancreatic cancer [54], colorectal cancer [55], prostate cancer [56], and also breast cancer [57] as a therapeutic biomarker. There is no study that has been exposed yet that hsa-mir-590-3p may play any role in OC.…”

Section: Discussionmentioning

confidence: 99%

Explore Novel Biomarkers and Associated Key Pathways to Perform as Potential Prognostic Biomarkers and Therapeutic Targets in Oral Cancer

Akhter¹,

Kahtani²,

Sambawa³

et al. 2022

Preprint

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Background: Oral cancer (OC) is serious health concerning issue that has a high fatality rate. The oral cavity has seven kinds of OC, including the lip, tongue, and floor of the mouth, as well as the buccal, hard palate, alveolar, retromolar trigone, and soft palate. The goal of this study is to look into new biomarkers and important pathways that might be used as diagnostic biomarkers and therapeutic candidates in OC. Methods: Publicly available repository the Gene Expression Omnibus (GEO) was responsible to collect OC-related datasets. GSE74530, GSE23558, and GSE3524 microarray datasets were collected to apply analysis. Minimum cut-off criteria of |log fold-change (FC)| > 1 and adjusted p < 0.05 were applied to figure out the up-regulated and down-regulated differential expression genes (DEGs) from the three datasets. After that only common DEGs in all three datasets were collected to apply further analysis. Gene ontology (GO) and Pathway analysis were implemented to explore the functional behaviors of DEGs. Then protein-protein interaction (PPI) networks were built to identify the most performed genes, clustering algorithm was also implemented to identify complex parts of PPI. TF-miRNA networks were also constructed to study deeply about OC-associated DEGs. Finally, top gene performers from PPI networks were used to apply drug signature analysis. Results: After applying filtration and cut-off criteria 2508, 3377, and 670 DEGs were found for GSE74530, GSE23558, and GSE3524 respectively, and 166 common DEGs were found in every dataset. The GO annotation remarks that most of the DEGs were associated with the terms of type I interferon signaling pathway. The pathways of KEGG reported that the common DEGs are related to the Cell cycle and Influenza A. The PPI network holds 88 nodes and 492 edges and CDC6 had the highest number of connections. 4 clusters were identified from the PPI. Drug signatures doxorubicin and resveratrol showed high significance according to the hub genes. We anticipate that our bioinformatics research will aid in the definition of the pathophysiology and the development of new therapies for OC.

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“…Increasing evidences found that many miRNAs associated with the cryopreservation of sperm in mammals are related with fertility, the development of early embryo, and pregnancy. Among the 11 DEmiRs that we identified in human frozen-thawed sperm, hsa-miR-106b-5p ( Wei et al, 2017 ; He et al, 2020 ), hsa-miR-590-3p ( Shi X. et al, 2020 ), hsa-miR-328-3p ( Bai et al, 2021 ), and hsa-miR-140-5p ( Nie et al, 2019 ; Jiao et al, 2020 ) may cause a decreased fertilization rate in IVF and ICSI cases when using cryopreserved sperm since these miRNAs are all related to cell cycle and mitosis. miR-106b-5p is a key miRNA morula and blastocyst developmental state, having potential interactions with embryo-expressed genes ( Sanchez et al, 2021 ).…”

Section: Discussionmentioning

confidence: 99%

Effect of Sperm Cryopreservation on miRNA Expression and Early Embryonic Development

Zhang

et al. 2021

Front. Cell Dev. Biol.

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Although sperm preservation is a common means of personal fertility preservation, its effects on embryonic development potential need further investigation. The purpose of this study was to identify key microRNA (miRNA) in cryopreserved sperm and determine the changes of these miRNAs and their target genes during embryonic development using cryopreserved sperm. Moreover, the embryonic development potential of cryopreserved sperm was estimated in assisted reproductive technology (ART), where key miRNAs and target genes were validated in sperm and subsequent embryos. Clinical data of embryonic development from cryopreserved sperm indicated a significant decrease in fertilization rate in both in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) cases, as well as a reduction in blastocyst formation rate in ICSI cases. Meanwhile there was a significant increase in blocked embryo ratio of Day1, Day2, and Day3.5 embryos when frozen-thawed mouse sperm was used, compared with fresh mouse sperm, suggesting a potential negative effect of sperm cryopreservation on embryonic development. From frozen-thawed and fresh sperm in humans and mice, respectively, 21 and 95 differentially expressed miRNAs (DEmiRs) were detected. miR-148b-3p were downregulated in both human and mouse frozen-thawed sperm and were also decreased in embryos after fertilization using cryopreserved sperm. Target genes of miR-148b-3p, Pten, was identified in mouse embryos using quantitative real-time PCR (qRT-PCR) and Western blot (WB). In addition, common characters of cryopreservation of mouse oocytes compared with sperm were also detected; downregulation of miR-148b-3p was also confirmed in cryopreserved oocytes. In summary, our study suggested that cryopreservation of sperm could change the expression of miRNAs, especially the miR-148b-3p across humans and mice, and may further affect fertilization and embryo development by increasing the expression of Pten. Moreover, downregulation of miR-148b-3p induced by cryopreservation was conserved in mouse gametes.

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<p><em>Hsa-MiR-590-3p</em> Promotes the Malignancy Progression of Pancreatic Ductal Carcinoma by Inhibiting the Expression of p27 and PPP2R2A via G1/S Cell Cycle Pathway</p>

Cited by 9 publications

References 31 publications

Exploring the Novel Computational Drug Target and Associated Key Pathways of Oral Cancer

Exploring the Novel Computational Drug Target and Associated Key Pathways of Oral Cancer

Explore Novel Biomarkers and Associated Key Pathways to Perform as Potential Prognostic Biomarkers and Therapeutic Targets in Oral Cancer

Effect of Sperm Cryopreservation on miRNA Expression and Early Embryonic Development

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