<p>Plasma Exosomal Long Noncoding RNA lnc-SLC2A12-10:1 as a Novel Diagnostic Biomarker for Gastric Cancer</p>

Abstract

Exosomes participate in cellular communications by transmitting active molecules, including long noncoding RNAs (lncRNAs) and are regarded as suitable candidates for disease diagnosis. This study aimed to identify gastric cancer (GC)-specific exosomal lncRNA and investigate the potential diagnostic value of plasma exosomal lncRNA in GC. Patients and Methods: Exosomes from the culture media (CM) of four GC cells (GCCs) and human gastric epithelial cells were isolated. Exosomal RNA was extracted, and lncRNA microarray assay was performed to identify GC-specific exosomal lncRNAs. The expression levels of the candidate exosomal lncRNAs were validated in 120 subjects via quantitative reverse transcription PCR (qRT-PCR). The receiver operating characteristic (ROC) curve and area under curve were used to estimate the diagnostic capacity. We investigated the potential relationship between plasma exosomal lncRNA expression and the clinicopathological parameters of GC. Results: A total of 199 exosomal lncRNAs were expressed at considerable higher levels in GCCs than those in normal controls, among which the top 10 upregulated lncRNAs were selected for further validation in cell, CM, and plasma. qRT-PCR revealed that lnc-SLC2A12-10:1 was remarkably upregulated in exosomes derived from patients with GC and GCCs. The area under the ROC curve was 0.776, which was higher than the diagnostic accuracies of CEA, CA 19-9, and CA72-4. The expression level of exosomal lnc-SLC2A12-10:1 was also significantly correlated with tumor size, TNM stage, lymph node metastasis, and degree of differentiation. The postoperative expression levels of exosomal lnc-SLC2A12-10:1 were lower compared with those of preoperative levels. Conclusion: Our study suggested that exosomal lnc-SLC2A12-10:1 may be a potential noninvasive biomarker for the diagnosis and prognosis monitoring of GC. Further large-scale studies are necessary to validate its performance in GC progression.

Insights Into Exosomal Non-Coding RNAs Sorting Mechanism and Clinical Application

“…Serum exosomal miRNA profiles of steroid-induced osteonecrosis of the femoral head (SONFH) and hsa-miR-135b-5p may be a unique diagnostic biomarker for SONFH (131). Zheng et al found that the expression level of exosomal lnc-SLC2A12-10:1 was significantly correlated with tumor size, TNM stage, lymph node metastasis, and degree of differentiation, suggesting that exosomal lnc-SLC2A12-10:1 may be a potential noninvasive biomarker for the diagnosis and prognosis monitoring of gastric cancer (132). In summary, ncRNAs derived from exosomes are considered potential new biomarkers for various diseases, especially cancer, and can be easily detected in liquid biopsies.…”

Role of Exosomal Non-coding RNAs in Gastric Cancer: Biological Functions and Potential Clinical Applications

“…Their AUCs were 0.849 and 0.776, respectively, which were higher than the diagnostic efficiencies of traditional diagnostic markers (CEA, CA19-9, and CA72-4). Furthermore, their expression levels were significantly correlated with the TNM stage (173,174).…”

<p>Exosomal Transfer of Macrophage-Derived miR-223 Confers Doxorubicin Resistance in Gastric Cancer</p>

“…The morphology and size distribution of the isolated exosomes were determined using transmission electron microscopy (TEM) and nanoparticle tracking analysis (NTA) following our previous description. 25 , 26 …”

“…The morphology and size distribution of the isolated exosomes were determined using transmission electron microscopy (TEM) and nanoparticle tracking analysis (NTA) following our previous description. 25,26 Cell Viability and Apoptosis Assay GC cells were seeded in a 96-well plate at 1500-3000 cells per well and incubated with doxorubicin for 48 h. Cell viability was detected with Cell Counting Kit-8 (Dojindo Laboratories, Japan). The optical density at 450 nm was measured on a multiwall plate reader (FLX800, Bio-TEK).…”