&lt;title&gt;Waveguide scattering microscopy in air and water&lt;/title&gt;

Thoma, Frank; Armitage, John C.; Trembley, Hugues; Menges, Bernhard; Langbein, U.; Mittler‐Neher, Silvia

doi:10.1117/12.323536

Cited by 7 publications

(4 citation statements)

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“…A digital camera (Diagnostic Instruments Inc, USA) and imaging software (Image Pro Express, Media Cybernetics) were used for image capture over a range of exposure times and for image analysis. A schematic of the set up can be found in [24].…”

Section: The Waveguide Evanescent Field Scattering Microscopy Set-upmentioning

confidence: 99%

“…The waveguide evanescent field scattering technique was developed by Thoma et al [23,24] for technical structures on surfaces using ion exchanged waveguides. Later, Waveguide Evanescent Field Fluorescence (WEFF) microscopy was developed [25][26][27][28][29] as a straightforward alternative to (TIRF) microscopy for imaging ultrathin films and cell-substrate interaction using fluorescence dyes located in the plasma membrane.…”

Section: Introductionmentioning

confidence: 99%

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Waveguide evanescent field scattering microscopy: bacterial biofilms and their sterilization response via UV irradiation

Nahar

Fleißner

Shuster

et al. 2013

Journal of Biophotonics

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Waveguide Evanescent Field Scattering (WEFS) microscopy is introduced as a new and simple tool for label-free, high contrast imaging of bacteria and bacteria sensors. Bacterial microcolonies and single bacteria were discriminated both by their bright field images and by their evanescent scattering intensity. By comparing bright field images with WEFS images, the proportion of planktonic: sessile (i.e., "floating": attached) bacteria were measured. Bacteria were irradiated with UV light, which limited their biofilm forming capability. A quantitative decrease in attachment of individual, sessile bacteria and in attached, microcolony occupied areas was easily determined within the apparent biofilms with increasing UV dose. WEFS microscopy is an ideal tool for providing rapid quantitative data on biofilm formation.

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Section: The Waveguide Evanescent Field Scattering Microscopy Set-upmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Waveguide evanescent field scattering microscopy: bacterial biofilms and their sterilization response via UV irradiation

Nahar

Fleißner

Shuster

et al. 2013

Journal of Biophotonics

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“…A scheme of the experimental setup can be found in Refs. 16 and 25. The accuracy in measuring the synchronous angles using this method is about Ϯ1Ј.…”

Section: M-line Spectroscopy With Prism Couplingmentioning

confidence: 99%

“…We have established an ion-exchange protocol to fabricate waveguides with a defined number of modes. With the help of m-line spectroscopy, 16,17 we characterized the fabricated waveguides with respect to the effective refractive indices, the effects of exchange-time and temperature, and the diffusion profiles and the temperature-dependent diffusion coefficient of the silver ions. The penetration depths of the evanescent field for different modes were calculated.…”

Section: Introductionmentioning

confidence: 99%

Optical waveguides formed by silver ion exchange in Schott SG11 glass for waveguide evanescent field fluorescence microscopy: evanescent images of HEK293 cells

et al. 2010

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Planar glass waveguides with a specific number of modes were fabricated by Ag(+)-Na(+) exchange in Schott SG11 glass. The effective refractive indices were determined using m-line spectroscopy in both s- and p-polarization. By using the reversed Wentzel-Kramers-Brillouin approximation, the index profiles were described by a nonlinear diffusion equation. The diffusion coefficients for Ag(+) were established, as well as the penetration depth of the evanescent field in an aqueous environment for the different modes. The integrals of \E\(2) fields for the evanescent-guided fields were investigated. These are important when evanescent fields are used for illumination in interface microscopy, an alternative method to total internal reflection fluorescence (TIRF) microscopy. The photoluminescent behavior of the waveguides was investigated as a function of ion exchange time and excitation wavelengths. Comparable images were obtained of fluorescently labeled HEK293 cells using TIRF microscopy and waveguide evanescent field fluorescence microscopy. Imaging was performed using HEK293 cells, delivering similar images and information.

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Imaging of Thin Films, and Its Application in the Life Sciences

Mittler

2010

Nanotechnologies for the Life Sciences

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This chapter summarizes the fabrication, patterning, microscopy, and applications of thin films and, notably, their applications in the life sciences. Because the field is enormous, only organic thin films on metal and glass substrates are described. Details of optical microscopy technologies are discussed in detail, based mainly on evanescent technology, while near‐field methods and electron microscopy are referred to only briefly. The applications described incorporate only a small fraction of those used in thin film technology and engineering in the life sciences.

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<title>Waveguide scattering microscopy in air and water</title>

Cited by 7 publications

References 7 publications

Waveguide evanescent field scattering microscopy: bacterial biofilms and their sterilization response via UV irradiation

Waveguide evanescent field scattering microscopy: bacterial biofilms and their sterilization response via UV irradiation

Optical waveguides formed by silver ion exchange in Schott SG11 glass for waveguide evanescent field fluorescence microscopy: evanescent images of HEK293 cells

Imaging of Thin Films, and Its Application in the Life Sciences

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