Luminescent bacterial sensor for cadmium and lead

Tauriainen, Sisko; Karp, Matti; Wu, Chang; Virta, Marko

doi:10.1016/s0956-5663(98)00027-x

Cited by 225 publications

(157 citation statements)

References 32 publications

Supporting

Mentioning

153

Contrasting

Unclassified

Order By: Relevance

“…Experiments showed that after a 2-h induction, the biosensor produced an obvious increase of RLU at 0.05 μM As (LOD), which was below the WHO permissible level of 10 μg/L (equivalent to 0.13 μM) in potable water (Nordstrom 2002). In addition, other factors such as the host strain, concentration of host strain, culture medium, and pH could also affect the LOD of a biosensor (Golding et al 2008;Rasmussen et al 1997;Riether et al 2001;Tauriainen et al 1998).…”

Section: Application Of Biosensor To Assess the Bioavailable As In DImentioning

confidence: 99%

The impacts of different long-term fertilization regimes on the bioavailability of arsenic in soil: integrating chemical approach with Escherichia coli arsRp::luc-based biosensor

Hou

et al. 2014

Appl Microbiol Biotechnol

View full text Add to dashboard Cite

An Escherichia coli arsRp::luc-based biosensor was constructed to measure the bioavailability of arsenic (As) in soil. In previous induction experiments, it produced a linear response (R 2 =0.96, P<0.01) to As from 0.05 to 5 μmol/L after a 2-h incubation. Then, both chemical sequential extraction, Community Bureau of Reference recommended sequential extraction procedures (BCR-SEPs) and E. coli biosensor, were employed to assess the impact of different long-term fertilization regimes containing N, NP, NPK, M (manure), and NPK+ M treatments on the bioavailability of arsenic (As) in soil. Per the BCR-SEPs analysis, the application of M and M+NPK led to a significant (P<0.01) increase of exchangeable As (2-7 times and 2-5 times, respectively) and reducible As (1.5-2.5 times and 1.5-2.3 times, respectively) compared with the no fertilization treated soil (CK). In addition, direct contact assay of E. coli biosensor with soil particles also supported that bioavailable As in manure-fertilized (M and M+NPK) soil was significantly higher (P<0.01) than that in CK soil (7 and 9 times, respectively). Organic carbon may be the major factor governing the increase of bioavailable As. More significantly, E. coli biosensor-determined As was only 18.46-85.17 % of exchangeable As and 20.68-90.1 % of reducible As based on BCR-SEPs. In conclusion, NKP fertilization was recommended as a more suitable regime in As-polluted soil especially with high As concentration, and this E. coli arsRp::luc-based biosensor was a more realistic approach in assessing the bioavailability of As in soil since it would not overrate the risk of As to the environment.

show abstract

Section: Application Of Biosensor To Assess the Bioavailable As In DImentioning

confidence: 99%

The impacts of different long-term fertilization regimes on the bioavailability of arsenic in soil: integrating chemical approach with Escherichia coli arsRp::luc-based biosensor

Hou

et al. 2014

Appl Microbiol Biotechnol

View full text Add to dashboard Cite

show abstract

“…Novel lead-specific biosensors have been developed (22)(23)(24)(25)(26)(27). Lu et al (22,(26)(27)(28) developed a new biosensor for lead by combining the high selectivity of a DNAzyme with a molecular beacon strategy to achieve sensitive and quantitative fluorescent detection of Pb 2+ over a wide concentration range from 10 nM to 10 µM.…”

Section: Introductionmentioning

confidence: 99%

Miniaturized Lead Sensor Based on Lead-Specific DNAzyme in a Nanocapillary Interconnected Microfluidic Device

Chang

Tulock

Liu

et al. 2005

Environ. Sci. Technol.

124

View full text Add to dashboard Cite

A miniaturized lead sensor has been developed by combining a lead-specific DNAzyme with a microfabricated device containing a network of microfluidic channels that are fluidically coupled via a nanocapillary array interconnect. A DNAzyme construct, selective for cleavage in the presence of Pb 2+ and derivatized with fluorophore (quencher) at the 5′ (3′) end of the substrate and enzyme strands, respectively, forms a molecular beacon that is used as the recognition element. The nanocapillary array membrane interconnect is used to manipulate fluid flows and deliver the small-volume sample to the beacon in a spatially confined detection window where the DNAzyme is interrogated using laser-induced fluorescence detection. A transformed log plot of the fluorescent signal exhibits a linear response (r 2 ) 0.982) over a Pb 2+ concentration range of 0.1-100 µM, and a detection limit of 11 nM. The sensor has been applied to the determination of Pb 2+ in an electroplating sludge reference material, the result agreeing with the certified value within 4.9%. Quantitative measurement of Pb 2+ in this complex sample demonstrates the selectivity of this sensor scheme and points favorably to the application of such technologies to analysis of environmental samples. The unique combination of a DNAzyme with a microfluidic-nanofluidic hybrid device makes it possible to change the DNAzyme to select for other compounds of interest, and to incorporate multiple sensing systems within a single device for greater flexibility. This work represents the initial steps toward creation of a robust field sensor for lead in groundwater or drinking water.

show abstract

“…3. Regulation of the pzntA promoter by the ZntR protein pMOL28 and pMOL30 plasmids was the object of an insertion of the transposon Tn4431 containing the luxAB genes (Corbisier et al, 1993;Tauriainen et al, 1998). The detection limit of the strain obtained was 1mM of Pb 2+ against 30nM of Pb 2+ for mechanisms resulting from the less resistant bacteria such as Escherichia coli and Staphylococcus aureus.…”

Section: 3mentioning

confidence: 99%

“…Various developments are proposed or were proposed on the market such as: the BIOMET® kit developed from transcriptional fusions between the luxCDABE operon and various resistance systems to heavy metals of the Ralstonia metallidurans strain (Vito, Belgium, http://www.vito.be/). One other kit using the inducible bioluminescent strains for arsenic or mercury (Biologically Heavy metal Assay kit) is distributed by Aboatox company following the works of Tauriainen et al (1997Tauriainen et al ( , 1998. The team of S.Daunert (Lexinton, Irvine, the USA) developed an original microfluidic and analysis system integrated in a compact disc allowing arsenic detection with the arsenicsensitive E.coli pSD10 (parsR:: arsR:: gfpuv) (Rothert et al, 2005 (Riether, Dollard et al, 2001) DL E.coli pJAMA-arsR (Stocker et al, 2003) Cd …”

Section: 3mentioning

confidence: 99%

Bacterial Bioluminescent Biosensor Characterisation for On-line Monitoring of Heavy Metals Pollutions in Waste Water Treatment Plant Effluents

Charrier¹,

Durand²,

Affi³

et al. 2010

Biosensors

View full text Add to dashboard Cite

Luminescent bacterial sensor for cadmium and lead

Cited by 225 publications

References 32 publications

The impacts of different long-term fertilization regimes on the bioavailability of arsenic in soil: integrating chemical approach with Escherichia coli arsRp::luc-based biosensor

The impacts of different long-term fertilization regimes on the bioavailability of arsenic in soil: integrating chemical approach with Escherichia coli arsRp::luc-based biosensor

Miniaturized Lead Sensor Based on Lead-Specific DNAzyme in a Nanocapillary Interconnected Microfluidic Device

Bacterial Bioluminescent Biosensor Characterisation for On-line Monitoring of Heavy Metals Pollutions in Waste Water Treatment Plant Effluents

Contact Info

Product

Resources

About