Luminescent Iridium(III)-Containing Block Copolymers: Self-Assembly into Biotin-Labeled Micelles for Biodetection Assays

Abstract

Supporting information Contents 1. Experimental section: Reagents, methods and equipment 2. Synthesis of PEG-Biotin ROMP monomer 5 3. General procedure for polymerisation 4. Synthesis and characterization of Polymer 6 : (PEG) 3 -(Ir) 10 -(nBu) 10 5. Synthesis and characterization of Polymer 7 : (Bio) 1 -(PEG) 3 -(Ir) 10 -(nBu) 10 6. Synthesis and characterization of Polymer 8 : (BioPEG) 3 -(Ir) 10 -(nBu) 10 7. TEM images of micelles from polymers 6, 7 and 8 8. AFM images of micelles from polymers 6 9. Quantitation of amount of polymer bound to Streptavidin-coated magnetic beads 10. Scheme for microcontact printing of Streptavidin onto glass surfaces 1. Experimental section: Reagents, methods and equipment Reagents and supplies.

Structure, function, self-assembly, and applications of bottlebrush copolymers

“…A series of bottlebrush polymers and block copolymers with different exterior and interior structures has been demonstrated: bottlebrushes with hydrophilic exteriors and hollow interiors 95,124 , bottlebrushes with covalently attached drug molecules 125,126,136 , and Janustype amphiphilic bottlebrushes 128 . Bottlebrush polymers and copolymers have been shown to provide on-demand drug release 137 with the capability for targeting through molecular recognition 133 .…”

“…Fluorescence intensity was significantly higher for bottlebrushes with the dye attached by a long side-chain compared with a short tether, and hydrophilic targeting agents were found to be more effective compared with hydrophobic targeting agents 41 . Sleiman et al prepared biotin-functionalized, luminescent bottlebrush copolymer assemblies for biodetection assays 132,133 . The bottlebrush copolymers reported contained a hydrophilic PEG bottlebrush block, biotin functionality for binding to streptavidin, and luminescent ruthenium, iridium, or osmium polypyridine complexes (Fig 13B).…”

“…These bottlebrush block copolymers self-assembled to form spherical micelles in solution with the luminescent metals in the core and hydrophilic PEG in the corona along with biotin groups for detection. Sleiman et al showed that placing biotin functionality on the periphery of the sidechains was necessary to get significant binding to streptavidin immobilized on beads or surfaces 133 .…”

Bottom‐Up Characterization and Self‐Assembly of Electrogenerated Chemiluminescence Active Ruthenium Nanospheres

“…Grubbs catalyst's living polymerization facilitates the creation of homogeneous regions of monomers, i. e. blocks, that are ideally suited for probing the effects of the polymer environment on luminophore emission . The tunability of the block's size and functionality enables readily altering the overall polymer structure . The integration of monomers containing hydrophobic and hydrophilic functional groups to block copolymers has been shown to thermodynamically drive the copolymers to self‐assemble when exposed to aqueous environments …”

Síntese de monômeros luminescentes com norborneno e suas polimerizações via metátese

“…9 Essas características permitiram que se desenvolvesem uma variedade de complexos alterando sua esfera de coordenação de maneira a modular suas propriedades eletroquímica e óptica, levando a aplicações como fotosenssibilizador para conversão de energia solar, sondas biológicas, fotocatálise e etc. 10,11 Graztel e colaboradores na década de 90 desenvolveram celulas solares, usando …”