Lung Disease Diagnostic Model Through IgG Sensitization to Microbial Extracellular Vesicles

Yang, Jinho; Hong, Goohyeon; Kim, Youn Seup; Seo, Ho-Chan; Kim, Sung-Won; McDowell, Andrea; Lee, Won Hee; Kim, You Sun; Oh, Yeon Mok; Cho, You Sook; Choi, Young Woo; Kim, You Young; Jee, Young Koo; Kim, Yoon Keun

doi:10.4168/aair.2020.12.4.669

Cited by 19 publications

(26 citation statements)

References 50 publications

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“…This information should contribute to knowledge of the involvement of lung microbiome in the development of COPD. Yang et al [ 39 ] demonstrated that the levels of antibacterial EV IgG antibodies were higher in patients with asthma and COPD compared to the healthy controls and we provided a lung disease diagnostic model based on antibacterial EV antibody titers.…”

Section: Summary and Discussionmentioning

confidence: 96%

“…Yang et al [ 39 ] performed microbiome analysis of indoor dust EVs isolated from mattresses in apartments and hospitals. We developed diagnostic models based on the bacterial EVs antibodies detected in serum samples via enzyme-linked immunosorbent assay.…”

Section: Main Findingsmentioning

confidence: 99%

“…Extracellular miRNAs may be useful as biomarkers for allergic disease, with the ability to classify disease subtype or activity, and that biologically relevant extracellular miRNAs may contribute to the pathogenesis of allergic inflammation and asthma [ 57 ]. Yang et al [ 39 ] provided a lung disease diagnostic model based on antibacterial EV antibody titers.…”

Section: Main Findingsmentioning

confidence: 99%

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Importance of microbial extracellular vesicle in the pathogenesis of asthma and chronic obstructive pulmonary disease and its diagnostic potential

Kim

Joh

Lee

2020

Asia Pacific Allergy

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There are rising evidences of the human microbiome as a potentially influential player that is actively engaged in shaping the pathogenetic processes and other unresolved issues both in asthma and other chronic respiratory diseases, particularly of the airways. The biological components such as microbiome in inhaled air can induce immune dysfunction and inflammation, leading to inflammatory pulmonary disorders such as asthma and chronic obstructive pulmonary disease (COPD). Microbe-derived extracellular vesicles (EVs) with biologically active information or functions can reprogram their respective target cells and EV may have a role for the development of asthma and COPD. To evaluate the role of microbederived EV in the pathogenesis of asthma and COPD and its role in diagnosis, the PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-Analyses) statement method was used for the study. An electronic search was performed using PubMed, PubMed Central, and Embase up to 2020. EVs serve as an intercellular transporter of miRNAs for cell-to-cell communication in the lungs. Bacteria-derived EVs have distinctive characteristics in the lungs of patients with asthma and COPD compared to healthy controls. Furthermore, bacterial EV IgG antibody titers in serum were significantly higher in patients with asthma and COPD than in healthy controls, suggesting that antibacterial EV antibodies titers can be used as a diagnostic tool for lung disease. Taken together, microbial EVs and miRNAs have important roles in the pathogenesis of asthma and COPD and they can provide novel diagnostic biomarkers for asthma and COPD.

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Section: Summary and Discussionmentioning

confidence: 96%

Section: Main Findingsmentioning

confidence: 99%

Section: Main Findingsmentioning

confidence: 99%

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Importance of microbial extracellular vesicle in the pathogenesis of asthma and chronic obstructive pulmonary disease and its diagnostic potential

Kim

Joh

Lee

2020

Asia Pacific Allergy

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“…Four studies [31][32][33][34] investigated whether bacteria-derived EVs were related to COPD (Table 5a). Three research articles [32][33][34] identi ed carried out ex vivo human studies and one study [31] was done in vivo using a mice model and additionally in vitro assays. Kim, Y.S., et al [31] determined that EVs derived from bacteria resulted in emphysema due to neutrophilic in ammation.…”

Section: Bacterial Evs and Copdmentioning

confidence: 99%

“…Three [32][33][34] of the studies were assessed and scores ranged from 0.50 to 0.64. Breakdown of their assessment can be noted in table 5b.…”

Section: Bacterial Evs and Copdmentioning

confidence: 99%

Extracellular Vesicles and Chronic Obstructive Pulmonary Disease (COPD): A Systematic Review

Gómez¹,

James²,

Onion³

et al. 2021

Preprint

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Background: Chronic Obstructive Pulmonary Disease (COPD) is a common inflammatory disease of the airways characterized by irreversible airflow limitation, ranking the third highest cause of death worldwide. Extracellular vesicles (EVs) are important intercellular communication mediators released by cells into their extracellular environment with the capacity to transfer biological signals. EVs involved in COPD hold great potential to understand disease pathogenesis and identify important biomarkers. This systematic review aims to examine all available research on EVs in the pathogenesis and diagnosis of COPD to identify existing knowledge and support further research within the field. Methods: Publications were searched using PubMed and EMBASE with the search terms (Exosomes or extracellular vesicles or microvesicles or microparticles or ectosomes) AND (chronic obstructive pulmonary disease or COPD or emphysema or bronchitis). Results: Initial search yielded 358 papers of which 94 were manually selected for review and 27 were eligible for analyses. The studies were divided into groups according to the role of EVs in pathogenesis, EV origin and cargo, their role in COPD exacerbations and their diagnostic utility. EVs were found to be involved in the mechanism of pathogenesis of COPD, derived from various cell types, as well as containing modified levels of miRNAs. EVs also varied according to the pathophysiological status of disease, therefore presenting a possible method for COPD diagnosis and progress monitoring. Conclusion: The current findings show the limited but good quality research looking at the role of EVs in COPD, demonstrating the need for more studies to better define and provide further insight into the functional characteristics of EV in COPD pathogenesis.

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Immunoregulatory effects of Lactococcus lactis‐derived extracellular vesicles in allergic asthma

Lee

Park

Lee³

et al. 2022

Clinical & Translational All

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Background Probiotics have been shown to prevent various allergic diseases by producing extracellular vesicles (EVs). However, the role of EVs in allergic asthma has not yet been completely determined. Methods Gut microbial composition, mainly genera related to probiotics, was investigated in allergic asthmatic mice. Moreover, EVs were isolated from Lactococcus lactis (L. lactis, a selected bacterium) and EV proteins were identified by peptide mass fingerprinting. EV functions in immune responses were evaluated in vivo or ex vivo. Furthermore, the levels of specific IgG antibodies (an alternative marker for EV quantification) to L. lactis‐EVs were measured by ELISA in the sera of 27 asthmatic patients and 26 healthy controls. Results Allergic asthmatic mice showed a lower proportion of Lactococcus compared to healthy mice. L. lactis was cultured and its EVs abundantly contained pyruvate kinase. When allergic asthmatic mice were intranasally treated with EVs, airway hyperresponsiveness, eosinophil number, cytokine secretion, and mucus production were significantly decreased. Moreover, L. lactis‐EV treatment shifted immune responses from Th2 to Th1 by stimulating dendritic cells to produce IL‐12. In addition, significantly lower levels of serum specific IgG4 (but not IgG1) to L. lactis‐EVs were noted in asthmatic patients than in healthy controls. A positive correlation between the levels of EV‐specific IgG4 and FEV1 (%), but a negative correlation between the levels of EV‐specific IgG4 and IL‐13 were observed. Conclusion These findings suggest that L. lactis‐EVs may have immune‐regulating effects on airway inflammation mediated by dendritic cell activation, providing a potential benefit for allergic asthma.

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Lung Disease Diagnostic Model Through IgG Sensitization to Microbial Extracellular Vesicles

Cited by 19 publications

References 50 publications

Importance of microbial extracellular vesicle in the pathogenesis of asthma and chronic obstructive pulmonary disease and its diagnostic potential

Importance of microbial extracellular vesicle in the pathogenesis of asthma and chronic obstructive pulmonary disease and its diagnostic potential

Extracellular Vesicles and Chronic Obstructive Pulmonary Disease (COPD): A Systematic Review

Immunoregulatory effects of Lactococcus lactis‐derived extracellular vesicles in allergic asthma

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