Lung Endothelial Transcytosis

Jones, Joshua H.; Minshall, Richard D.

doi:10.1002/cphy.c190012

Cited by 21 publications

(22 citation statements)

References 345 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the case of nulls, the primary change may be above the band, whereas in mode-changes it is likely to be outside the observable band in a region of larger Φ ref with only secondary changes above the band. Periodicity is the only difference from the mode-change and null mechanisms described previously (Jones 2018(Jones , 2020. Pulse sequences observed by Yan et al have a well-defined LRFS frequency but do not appear as a high-Q system.…”

Section: Short Time-scalesmentioning

confidence: 77%

“…Hence, in general, the outward flux of accelerated particles is of protons, with ions making up any deficit in charge density to the Goldreich-Julian value. We refer to Jones (2010Jones ( , 2020 for more complete details.…”

Section: The Symmetric Polar Capmentioning

confidence: 99%

“…Some evaluations of the model have been published previously (Jones 2020) and have shown how the existence of τp gives an understanding of stochastic behaviour with short null lengths 10 2 s in normal pulsars. Recently, Mahajan et al (2018) have observed short-lived mode-changes with an average length of 1.7 s in MSP B1957+20.…”

Section: Short Time-scalesmentioning

confidence: 99%

“…Periodicity defined by τp is therefore very plausible but unfortunately the cellular evaluation of the model described by Jones (2020) is not suitable for its demonstration. This is also true in the case of subpulse drift, a phenomenon which has been described previously (Jones 2014) but only in outline within the ion-proton model.…”

Section: Short Time-scalesmentioning

confidence: 99%

“…The ion-proton polar cap with positive corotational charge density is a model open magnetosphere with the merit of supporting a common source of coherent radio emission in normal pulsars, millisecond pulsars (MSP) and longperiod pulsars such as J2144-3933. It also shows how nulls occur with varying rotation period and whole-surface temperature (Jones 2020). In these regards it has advantages over the canonical electron-positron pair models for which it is not even possible to show that pair creation of adequate multiplicity occurs in the majority of pulsars (Hibschman & Arons 2001;Harding & Muslimov 2002) unless special assumptions are made about multipole fields, which are implausible in the MSP and in the 8.5 s pulsar J2144-3933 (Young, Manchester & Johnston 1999).…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Complex emission patterns: fluctuations and bistability of polar-cap potentials

Jones

2020

Monthly Notices of the Royal Astronomical Society

View full text Add to dashboard Cite

Development of the ion-proton pulsar model extends it to the limit of large unscreened polar-cap potentials, for example, as in the Vela pulsar, in which ion charges differ only by small increments from their complete screening values. It is shown that the atomic number Z of an ion following its passage from the canonical Z 0 = 26 value through the electromagnetic shower region to the surface is not necessarily timeindependent but can vary between fixed limits in an irregular or quasi-periodic way in a characteristic time of the order of 10 4 s. Thus at a certain Z the system may transition to an unstable state of higher electric potential and it is argued that this is the physical basis for mode-changes, long-term nulls, periodic or otherwise. The model requires an orientation of magnetic dipole moment relative to rotational spin giving a positive corotational charge density. Success of the model would fix the particle composition of the remaining parts of the magnetosphere, including the Y-point and is therefore relevant to X-ray and γ-ray emission processes.

show abstract

Section: Short Time-scalesmentioning

confidence: 77%

Section: The Symmetric Polar Capmentioning

confidence: 99%

Section: Short Time-scalesmentioning

confidence: 99%

Section: Short Time-scalesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Complex emission patterns: fluctuations and bistability of polar-cap potentials

Jones

2020

Monthly Notices of the Royal Astronomical Society

View full text Add to dashboard Cite

show abstract

The menace of severe adverse events and deaths associated with viral gene therapy and its potential solution

Kachanov,

Kostyusheva,

Brezgin

et al. 2024

Medicinal Research Reviews

View full text Add to dashboard Cite

Over the past decade, in vivo gene replacement therapy has significantly advanced, resulting in market approval of numerous therapeutics predominantly relying on adeno‐associated viral vectors (AAV). While viral vectors have undeniably addressed several critical healthcare challenges, their clinical application has unveiled a range of limitations and safety concerns. This review highlights the emerging challenges in the field of gene therapy. At first, we discuss both the role of biological barriers in viral gene therapy with a focus on AAVs, and review current landscape of in vivo human gene therapy. We delineate advantages and disadvantages of AAVs as gene delivery vehicles, mostly from the safety perspective (hepatotoxicity, cardiotoxicity, neurotoxicity, inflammatory responses etc.), and outline the mechanisms of adverse events in response to AAV. Contribution of every aspect of AAV vectors (genomic structure, capsid proteins) and host responses to injected AAV is considered and substantiated by basic, translational and clinical studies. The updated evaluation of recent AAV clinical trials and current medical experience clearly shows the risks of AAVs that sometimes overshadow the hopes for curing a hereditary disease. At last, a set of established and new molecular and nanotechnology tools and approaches are provided as potential solutions for mitigating or eliminating side effects. The increasing number of severe adverse reactions and, sadly deaths, demands decisive actions to resolve the issue of immune responses and extremely high doses of viral vectors used for gene therapy. In response to these challenges, various strategies are under development, including approaches aimed at augmenting characteristics of viral vectors and others focused on creating secure and efficacious non‐viral vectors. This comprehensive review offers an overarching perspective on the present state of gene therapy utilizing both viral and non‐viral vectors.

show abstract

The Glycocalyx and Pressure-Dependent Transcellular Albumin Transport

Dull

Chignalia

2020

Cardiovasc Eng Tech

View full text Add to dashboard Cite

Purpose Acute increases in hydrostatic pressure activate endothelial signaling pathways that modulate barrier function and vascular permeability. We investigated the role the glycocalyx and established mechanotransduction pathways in pressure-induced albumin transport across rat lung microvascular endothelial cells. Methods Rat lung microvascular endothelial cells (RLMEC) were cultured on Costar Snapwell chambers. Cell morphology was assessed using silver nitrate staining. RLMEC were exposed to zero pressure (Control) or 30 cmH2O (Pressure) for 30 or 60 min. Intracellular albumin uptake and transcellular albumin transport was quantified. Transcellular transport was reported as solute flux (Js) and an effective permeability coefficient (Pe). The removal of cell surface heparan sulfates (heparinase), inhibition of NOS (L-NAME) and reactive oxygen species (apocynin, Apo) was investigated. Results Acute increase in hydrostatic pressure augmented albumin uptake by 30–40% at 60 min and Js and Pe both increased significantly. Heparinase increased albumin uptake but attenuated transcellular transport while L-NAME attenuated both pressure-dependent albumin uptake and transport. Apo interrupted albumin uptake under both control and pressure conditions, leading to a near total lack of transcellular transport, suggesting a different mechanism and/or site of action. Conclusion Pressure-dependent albumin uptake and transcellular transport is another component of endothelial mechanotransduction and associated regulation of solute flux. This novel albumin uptake and transport pathway is regulated by heparan sulfates and eNOS. Albumin uptake is sensitive to ROS. The physiological and clinical implications of this albumin transport are discussed.

show abstract

Lung Endothelial Transcytosis

Cited by 21 publications

References 345 publications

Complex emission patterns: fluctuations and bistability of polar-cap potentials

Complex emission patterns: fluctuations and bistability of polar-cap potentials

The menace of severe adverse events and deaths associated with viral gene therapy and its potential solution

The Glycocalyx and Pressure-Dependent Transcellular Albumin Transport

Contact Info

Product

Resources

About