Lung Organoids in Smoking Research: Current Advances and Future Promises

Agraval, Hina; Chu, Hong Wei

doi:10.3390/biom12101463

Cited by 10 publications

(5 citation statements)

References 184 publications

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“…Distal airways are the major site of pathology in deployment-related respiratory disease (3,29). However, cell culture and mouse models do not fully represent the microenvironment in human distal lungs or cannot reflect the (30)(31)(32)(33). By using this unique PCLS model, we found that PMa alone significantly increased AHR that is consistent with previous animal studies on the health effects of PM (27,34).…”

Section: Discussionsupporting

confidence: 88%

“…However, cell culture and mouse models do not fully represent the microenvironment in human distal lungs or cannot reflect the complexity of human physiological responses. The human lung PCLS model offers several major advantages over other models including intact tissue/organ architecture and native microenvironments ( 30–33 ). By using this unique PCLS model, we found that PMa alone significantly increased AHR that is consistent with previous animal studies on the health effects of PM ( 27 , 34 ).…”

Section: Discussionmentioning

confidence: 99%

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Desert particulate matter from Afghanistan increases airway obstruction in human distal lungs exposed to type 2 cytokine IL-13

et al. 2023

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IntroductionDeployment related asthma-like symptoms including distal airway obstruction have been described in U.S. military personnel who served in Iraq and Afghanistan. The mechanisms responsible for the development of distal airway obstruction in deployers exposed to desert particulate matter (PM) is not well understood. We sought to determine if respiratory exposure to PM from Afghanistan (PMa) increases human distal airway hyperresponsiveness (AHR) with or without exposures to IL-13, a type 2 cytokine. We further tested whether mitochondrial dysfunction, such as ATP signaling and oxidative stress, may contribute to PMa- mediated AHR.MethodsPrecision-cut lung slices from donors without a history of lung disease, tobacco smoking, or vaping were pre-treated with IL-13 for 24 h. This was followed by exposure to PMa or PM from California (PMc, control for PMa) for up to 72 h. The role of hydrogen peroxide and ATP in AHR was assessed using the antioxidant enzyme catalase or an ATP receptor P2Y13 antagonist MRS2211. AHR in response to methacholine challenges as well as cytokine IL-8 production were measured.ResultsPMa alone, but not PMc alone, trended to increase AHR. Importantly, the combination of PMa and IL-13 significantly amplified AHR compared to control or PMc+IL-13. PMa alone and in combination with IL-13 increased IL-8 as compared to the control. PMa increased H2O2 and ATP. MRS211 and catalase reduced AHR in PCLS exposed to both PMa and IL-13.DiscussionOur data suggests that PMa in a type 2 inflammation-high lung increased AHR in part through oxidative stress and ATP signaling.

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Section: Discussionsupporting

confidence: 88%

Section: Discussionmentioning

confidence: 99%

Desert particulate matter from Afghanistan increases airway obstruction in human distal lungs exposed to type 2 cytokine IL-13

et al. 2023

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“…Exposure to cigarette smoke remains a high-risk factor for COPD (22). It is reported that oxidative stress, such as that induced by CSE, plays an important role in the development of corticosteroid resistance in COPD (12,23).…”

Section: Discussionmentioning

confidence: 99%

Salidroside restores glucocorticoid sensitivity in chronic obstructive pulmonary disease by activating Nrf2/HO-1 pathway

Deng

Xie

Zheng

et al. 2023

Preprint

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Corticosteroid resistance is the essential obstacle for effective treatment of chronic obstructive pulmonary disease (COPD). Oxidative stress and persistent chronic inflammation caused by cigarette smoke are likely to induce this corticosteroid insensitivity. Salidroside (Sal) is a main ingredient of traditional Chinese medicine rhodiola, which has the effects of anti-oxidation and anti-inflammatory. We aimed to investigate the role of Sal in the corticosteroid sensitivity of COPD. Collecting peripheral blood mononuclear cells (PBMC) from healthy volunteers (HV) and patients with COPD, and human monocytic U937 cells stimulated by normal culture and cigarette smoke extract (CSE), corticosteroid sensitivity was determined as the dexamethasone concentration causing 30% inhibition of tumor necrosis factor alpha (TNF-α)-induced IL-8 production (Dex-IC ) in these cells. Compared with PBMC from HV and normally cultured U937, in PBMC from COPD patients and U937 cells stimulated by CSE, the inhibitory effect of dexamethasone on IL-8 was weakened and the levels of nuclear factor erythroid 2‐related factor 2 (Nrf2) and histone deacetylase‐2 (HDAC2) was reduced. In conclusion, Sal restored dexamethasone inhibition of IL‐8 production, accompanied by raising the expression of Nrf2 and HDAC2 levels. Sal may upregulate HDAC2 expression by activating the Nrf2/HO-1 signaling pathway, and then restore corticosteroid sensitivity in COPD.

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“…Lebih dari 50 zat yang terkandung di dalamnya dapat menyebabkan berbagai macam penyakit paru. 4,5 Jumlah kematian akibat merokok diperkirakan akan terus bertambah bahkan setelah tingkat penggunaan tembakau menurun, hal ini dikarenakan penyakit yang terkait dengan tembakau membutuhkan waktu yang lama untuk sembuh.6 Bahaya rokok telah banyak diketahui oleh masyarakat umum, namun tidak terjadi penurunan jumlah perokok di Indonesia, bahkan ada peningkatan di setiap tahunnya. Jumlah perokok yang semakin meningkat dan usia perokok semakin lama semakin muda.…”

Section: Pendahuluanunclassified

Pengetahuan Penyakit Pernapasan Kronik pada Perokok Aktif

Laksono,

Suryadinata,

Lorensia

2023

comphi

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Cigarettes are one of the addictive substances in the form of nicotine, which can cause dependence for the wearer.In Indonesia, the number of smokers continues to increase every year. The prevalence of adult smokers consistedof men (67.4%), women (4.5%) from 36.1% of the population. The mortality rate due to smoking is expected tocontinue to increase because there is a significant relationship between the degree of smoking and the severity ofCOPD. COPD is a disease that attacks the lungs, one of the causes of which is cigarette smoke. The higher the levelof knowledge of active smokers, the lower the risk of a person getting COPD. Therefore, research is neededregarding the level of knowledge of chronic respiratory diseases related to active smokers. This study aims todetermine the level of knowledge of chronic respiratory disease in active smokers in Pungging Mojokerto District.The method used is a quantitative cross-sectional design with purposive sampling and analyzed descriptively. Theresults showed that the level of knowledge of active smokers in the District of Pungging Mojokerto on chronicrespiratory disease there are 3 levels of categories. Of the three categories, the highest level of knowledge wasobtained in the moderate category (50.82%), high category (44.26%), and low category (3.28%).

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Lung Organoids in Smoking Research: Current Advances and Future Promises

Cited by 10 publications

References 184 publications

Desert particulate matter from Afghanistan increases airway obstruction in human distal lungs exposed to type 2 cytokine IL-13

Desert particulate matter from Afghanistan increases airway obstruction in human distal lungs exposed to type 2 cytokine IL-13

Salidroside restores glucocorticoid sensitivity in chronic obstructive pulmonary disease by activating Nrf2/HO-1 pathway

Pengetahuan Penyakit Pernapasan Kronik pada Perokok Aktif

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