Lupeol acetate production and antioxidant activity of a cell suspension culture from Cnidoscolus chayamansa leaves

Pérez-González, Mariana Z.; Nieto-Trujillo, Aurelio; Gutiérrez-Rebolledo, Gabriel Alfonso; García-Martínez, Ignacio; Estrada-Zúñiga, María Elena; Bernabé-Antonio, Antonio; Jiménez-Arellanes, María Adelina; Cruz-Sosa, Francisco

doi:10.1016/j.sajb.2019.06.030

Cited by 20 publications

(9 citation statements)

References 39 publications

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“…Oxidative Medicine and Cellular Longevity to the potential of A. aculeata extracts in the antioxidant assays, as already reported in some studies with other plants [36,48,[53][54][55]. GC-MS analysis also identified campesterol, stigmasterol, and β-sitosterol, as well as the triterpenes lupeol and lupeol acetate, which are known to possess antioxidant properties [56] and anti-inflammatory effects [57]. These molecules have been investigated with pharmacological purposes in cancer, diabetes, and cardiovascular diseases [58].…”

mentioning

confidence: 56%

Acrocomia aculeata(Jacq.) Lodd. ex Mart. Leaves Increase SIRT1 Levels and Improve Stress Resistance

Alfredo

Matafome

Iacia

et al. 2020

Oxidative Medicine and Cellular Longevity

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Oxidative stress is a metabolic disorder linked with several chronic diseases, and this condition can be improved by natural antioxidants. The fruit pulp of the palm Acrocomia aculeata (Jacq.) Lodd. ex Mart. is widely used in the treatment of various illnesses, but as far as we know, there are no reports regarding the properties of its leaves. Thus, we aimed to evaluate the antioxidant activity of A. aculeata leaf extracts obtained with water (EA-Aa), ethanol (EE-Aa), and methanol (EM-Aa) solvents. The extracts were chemically characterized, and their antioxidant activity was assessed through the scavenging of the free radicals DPPH and ABTS. EE-Aa and EM-Aa showed the highest amounts of phenolic compounds and free radical scavenging activity. However, EA-Aa was more efficient to protect human erythrocytes against AAPH-induced hemolysis and lipid peroxidation. Thus, we further show the antioxidant effect of EA-Aa in preventing AAPH-induced protein oxidation, H2O2-induced DNA fragmentation, and ROS generation in Cos-7 cells. Increased levels of Sirt1, catalase, and activation of ERK and Nrf2 were observed in Cos-7 treated with EA-Aa. We also verify increased survival in nematodes C. elegans, when induced to the oxidative condition by Juglone. Therefore, our results showed a typical chemical composition of plants for all extracts, but the diversity of compounds presented in EA-Aa is involved in the lower toxicity and antioxidant properties provided to the macromolecules tested, proteins, DNA, and lipids. This protective effect also proven in Cos-7 and in C. elegans was probably due to the activation of the Sirt1/Nrf2 pathway. Altogether, the low toxicity and the antioxidant properties of EA-Aa showed in all the experimental models support its further use in the treatment of oxidative stress-related diseases.

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confidence: 56%

Acrocomia aculeata(Jacq.) Lodd. ex Mart. Leaves Increase SIRT1 Levels and Improve Stress Resistance

Alfredo

Matafome

Iacia

et al. 2020

Oxidative Medicine and Cellular Longevity

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“…In addition, sterols have been found between 10.2% to 13.0%, mainly β -sitosterol at 6.6% to 12.1% [ 40 ]. In a study, we found that in extracts with a mixture of chloroform and methanol (1:1) of Cnidoscolus chayamansa cell suspension cultures, lupeol acetate was obtained (38.1 mg/g extract), and the extracts had antibacterial activity [ 41 ]. Nonanedioic acid, β -sitosterol, stigmasterol, stigmasta-3,5-dien-7-one, β -amyrin, ( Z,Z )-9,12-octadecadienoic acid, D-pinitol, hexadecanoic acid, and other compounds have been reported for Eysenhardtia genus and some Fabaceae species [ 10 , 11 , 42 ]; however, in this work, the phytochemical screening by GC‒MS analysis of plants and cell suspension culture extracts from E. platycarpa is reported for the first time.…”

Section: Resultsmentioning

confidence: 99%

Establishment of a Cell Suspension Culture of Eysenhardtia platycarpa: Phytochemical Screening of Extracts and Evaluation of Antifungal Activity

Bernabé-Antonio

Sánchez-Sánchez

Romero-Estrada

et al. 2021

Plants

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Eysenhardtia platycarpa (Fabaceae) is a medicinal plant used in Mexico. Biotechnological studies of its use are lacking. The objective of this work was to establish a cell suspension culture (CSC) of E. platycarpa, determine the phytochemical constituents by spectrophotometric and gas chromatography‒mass spectrometry (GC‒MS) methods, evaluate its antifungal activity, and compare them with the intact plant. Friable callus and CSC were established with 2 mg/L 1-naphthaleneacetic acid plus 0.1 mg/L kinetin. The highest total phenolics of CSC was 15.6 mg gallic acid equivalents (GAE)/g dry weight and the total flavonoids content ranged from 56.2 to 104.1 µg quercetin equivalents (QE)/g dry weight. The GC‒MS analysis showed that the dichloromethane extracts of CSC, sapwood, and heartwood have a high amount of hexadecanoic acid (22.3‒35.3%) and steroids (13.5‒14.7%). Heartwood and sapwood defatted hexane extracts have the highest amount of stigmasterol (~23.4%) and β-sitosterol (~43%), and leaf extracts presented β-amyrin (16.3%). Methanolic leaf extracts showed mostly sugars and some polyols, mainly D-pinitol (74.3%). Compared with the intact plant, dichloromethane and fatty hexane extracts of CSC exhibited percentages of inhibition higher for Sclerotium cepivorum: 71.5% and 62.0%, respectively. The maximum inhibition for Rhizoctonia solani was with fatty hexane extracts of the sapwood (51.4%). Our study suggests that CSC extracts could be used as a possible complementary alternative to synthetic fungicides.

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“…In addition, sterols have been found between 10.2% to 13.0%, mainly β-sitosterol at 6.6% to 12.1% [40]. In a study, we found that in extracts with a mixture of chloroform and methanol (1:1) of Cnidoscolus chayamansa cell suspension cultures, lupeol acetate was obtained (38.1 mg/g extract), and the extracts had antibacterial activity [41]. Nonanedioic acid, β-sitosterol, stigmasterol, stigmasta-3,5-dien-7-one, βamyrin, (Z,Z)-9,12-octadecadienoic acid, D-pinitol, hexadecanoic acid, and other compounds have been reported for Eysenhardtia genus and some Fabaceae species [10,11,42]; however, in this work, the phytochemical screening by GC-MS analysis of plants and cell suspension culture extracts from E. platycarpa is reported for the first time.…”

Section: Antifungal Activitymentioning

confidence: 92%

Establishment of a Cell Suspension Culture of Eysenhardtia platycarpa: Phytochemical Screening of extracts and Evaluation of Antifungal Activity.

Bernabé-Antonio¹,

Sánchez-Sánchez²,

Romero-Estrada³

et al. 2020

Preprint

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Eysenhardtia platycarpa (Fabaceae) is a medicinal plant used in México and it lacks biotechnological studies for its use. The aim of this work was to establish a cell suspension cultures (CSC) of E. platycarpa, determine the phytochemical profile, and evaluate its antifungal activity. Friable callus and CSC were established with 2 mg/L 1-naphthaleneacetic acid plus 0.1 mg/L kinetin. The highest total phenolics of CSC was 15.6 mg GAE/g dry weight and the total flavonoids content ranged from 56.2 to 104.1 µg QE/g dry weight. CG‒MS analysis showed that the dichloromethane extracts of CSC, sapwood and heartwood have a high amount of hexadecanoic acid (22.3 ‒ 35.3 %) and steroids (13.5 ‒ 14.7%). Heartwood and sapwood defatted hexane extracts have the highest amount of stigmasterol (≈ 23.4%) and β-sitosterol (≈ 43%), and leaf extracts presented β-amyrin (16.3%). Methanolic leaves extracts showed mostly sugars and some polyols, mainly D-pinitol (74.3%). Dichloromethane and fatty hexane extracts of CSC exhibited the percentages inhibition higher for Sclerotium cepivorum with 71.5 and 62.0%, respectively. The maximum inhibition for Rhizoctonia solani was with fatty hexane extracts of the sapwood (51.4%). Our study suggest that CSC extracts could be used as a possible complementary alternative to synthetic fungicides.

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Lupeol acetate production and antioxidant activity of a cell suspension culture from Cnidoscolus chayamansa leaves

Cited by 20 publications

References 39 publications

Acrocomia aculeata(Jacq.) Lodd. ex Mart. Leaves Increase SIRT1 Levels and Improve Stress Resistance

Acrocomia aculeata(Jacq.) Lodd. ex Mart. Leaves Increase SIRT1 Levels and Improve Stress Resistance

Establishment of a Cell Suspension Culture of Eysenhardtia platycarpa: Phytochemical Screening of Extracts and Evaluation of Antifungal Activity

Establishment of a Cell Suspension Culture of Eysenhardtia platycarpa: Phytochemical Screening of extracts and Evaluation of Antifungal Activity.

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