Luteirhabdus pelagi gen. nov., sp. nov., a novel member of the family Flavobacteriaceae, isolated from the West Pacific Ocean

Abstract

A Gram-stain-negative, aerobic, and yellow-pigmented bacterium, designated A3-108T, was isolated from seawater of the West Pacific Ocean. Cells were non-motile and rod-shaped, with carotenoid-type pigments. Strain A3-108T grew at pH 6.0–8.5 (optimum 6.5) and 15–40 °C (optimum 28 °C), in the presence of 0.5–10% (w/v) NaCl (optimum 1.0%). It possessed the ability to produce H2S. Based on the 16S rRNA gene analysis, strain A3-108T exhibited highest similarity with Aureisphaera salina A6D-50T (90.6%). Phylogenetic analysis shown that strain A3-108T affiliated with members of the family Flavobacteriaceae and represented an independent lineage. The principal fatty acids were iso-C15:0, iso-C17:0 3-OH, iso-C15:1 G, and summed feature 3 (C16:1ω7c and/or C16:1ω6c). The sole isoprenoid quinone was MK-6. The major polar lipids were phosphatidylethanolamine, one unidentified aminophospholipid, one unidentified aminolipid and one unidentified lipid. The ANIb, in silico DDH and AAI values among the genomes of strain A3-108T and three reference strains were 67.3–71.1%, 18.7–22.1%, and 58.8–71.4%, respectively. The G + C content was 41.0%. Distinctness of the phylogenetic position as well as differentiating chemotaxonomic and other phenotypic traits revealed that strain A3-108T represented a novel genus and species of the family Flavobacteriaceae, for which the name Luteirhabdus pelagi gen. nov., sp. nov. is proposed (type strain, A3-108T = CGMCC 1.18821T = KCTC 82563T).

Marixanthotalea marina gen. nov., sp. nov., a bacterium in the family Flavobacteriaceae isolated from seawater

“…The family Flavobacteriaceae accommodates bacteria that are non-motile or motile by gliding, Gram-stain-negative and characterized chemotaxonomically by the presence of menaquinone 6 and the absence of sphingophospholipids [1,2]. The family is found in various habitats, such as marine sediment, tidal flat sediment, Antarctic ocean, Arctic ocean, ice lake and red algae [8][9][10][11][12]. Members of the family occur in marine environments and form a well-defined 'marine clade' , which are shown to be important in degradation of organic matter and the nutrient cycles in marine ecosystems but also comprises some clinically significant species [13,14].…”

“…Members of the family occur in marine environments and form a well-defined 'marine clade' , which are shown to be important in degradation of organic matter and the nutrient cycles in marine ecosystems but also comprises some clinically significant species [13,14]. Most novel genera within this family described recently were isolated from marine environments [8][9][10]. The family Flavobacteriaceae also includes a rather complex group of halophilic organisms, many of which are psychrophilic.…”