Luteolin is an Effective Component of Platycodon grandiflorus in Promoting Wound Healing in Rats with Cutaneous Scald Injury

Abstract

Background Platycodon grandiflorus could significantly improve the pathological results of cutaneous scald injury, reduce the release of inflammatory factors and promote angiogenesis. This study investigated the wound healing effect of luteolin, an active component of P. grandiflorus , on induced cutaneous scald injury in Sprague-Dawley (SD) rats. Methods The protein expression levels of TNF-α and IL-6 were detected by ELISA. QRT-PCR was adopted to detect the expression of TGF-β1 and VEGF. Histopathological changes of scald wounds were analyzed by hematoxylin-eosin staining. Cell viability and migration ability were detected by CCK-8 assay and scratch assay. Results Both in vivo and in vitro experiments showed that luteolin promoted wound healing of cutaneous scald injury. Gene Oncology (GO) functional analysis and rescue experiments showed that endothelial nitric oxide synthase 3 (NOS3) was the critical target of luteolin in treating cutaneous scald. Conclusion This study demonstrated that luteolin is an effective component of P. grandiflorus and is effective in the treatment of cutaneous scald injury.

Ethnopharmacology, phytochemistry, pharmacology and product application of Platycodon grandiflorum: A review

“…Modern pharmacological studies have revealed that the main active ingredients in Jiegeng are triterpene saponins, flavonoids, and polysaccharides, exhibiting diverse biological activities such as anti-tumor, anti-tussive, expectorant, anti-inflammatory, anti-bacterial, and anti-oxidant properties ( Lee, Yoon, Kim, & Lim, 2004 ). Jiegeng also contains fatty acids, amino acids, vitamins, and other beneficial nutrients ( Wang et al, 2022a ). Beyond its medicinal applications, Jiegeng is widely incorporated as an edible ingredient in pickles, assorted dishes, sausages, preserved fruits, and health beverages in China, Japan, and Korea.…”