Lycopene Alleviates Titanium Dioxide Nanoparticle-Induced Testicular Toxicity by Inhibiting Oxidative Stress and Apoptosis in Mice

Meng, Xiaojia; Li, Li; An, Hongmei; Deng, Yaxin; Ling, Chunmei; Lu, Tianjiao; Song, Guanling; Wang, Yan

doi:10.1007/s12011-021-02881-1

Cited by 23 publications

(19 citation statements)

References 56 publications

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“…After reviewing the previous studies, we found that the concentration of lycopene in serum and the induction of antioxidant enzymes were inversely U‐shaped with the dose of lycopene given to rats (Breinholt et al, 2000; Wang et al, 2018). Combined with our previous research that lycopene at doses of 20 and 40 mg/kg BW could improve the testicular injury induced by nano‐TiO 2 (Meng et al, 2022), lycopene doses of 0, 5, 20, and 40 mg/kg BW were therefore selected to explore its protective effect on the hepatotoxicity of nano‐TiO 2 . In this study, we observed that different concentrations of lycopene had no effect on the body weight and the liver coefficient of the mice, but mitigated the hepatotoxicity caused by nano‐TiO 2 .…”

Section: Discussionmentioning

confidence: 99%

“…Combined with our previous research that lycopene at doses of 20 and 40 mg/kg BW could improve the testicular injury induced by nano-TiO 2 (Meng et al, 2022), lycopene doses of 0, 5, 20, and 40 mg/ kg BW were therefore selected to explore its protective effect on the hepatotoxicity of nano-TiO 2 . In this study, we observed that different concentrations of lycopene had no effect on the body weight and the liver coefficient of the mice, but mitigated the hepatotoxicity caused by nano-TiO 2 .…”

Section: Discussionmentioning

confidence: 99%

“…Western blot was performed as previously described (Meng et al, 2022). Briefly, the liver tissue samples were fully lysed in RIPA buffer that supplemented with protease inhibitors on ice.…”

Section: Western Blot Analysismentioning

confidence: 99%

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Protective effects of lycopene on TiO₂ nanoparticle‐induced damage in the liver of mice

Chang

Deng

et al. 2023

J of Applied Toxicology

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Titanium dioxide nanoparticles (nano‐TiO2) is one of the most widely used and produced nanomaterials. Studies have demonstrated that nano‐TiO2 could induce hepatotoxicity through oxidative stress, and lycopene has strong antioxidant capacity. The present study aimed to explore if lycopene protects the liver of mice from nano‐TiO2 damage. Ninety‐six ICR mice were randomly divided into eight groups. They were control group, nano‐TiO2‐treated group (50 mg/kg BW), lycopene‐treated groups (5, 20, and 40 mg/kg BW), and 50 mg/kg BW nano‐TiO2‐ and lycopene‐co‐treated groups (nano‐TiO2 + 5 mg/kg BW of lycopene, nano‐TiO2 + 20 mg/kg BW of lycopene, nano‐TiO2 + 40 mg/kg BW of lycopene). After treated by gavage for 30 days, the histopathology of the liver was observed. Liver function was evaluated using changes in serum biochemical indicators of the liver (AST, ALT, ALP); and the level of ROS was indirectly reflected by the level of SOD, GSH‐Px, MDA, GSH, and T‐AOC. TUNEL assay was performed to examine the apoptosis of hepatocytes. Proteins of p53, cleaved‐caspase 9, cleaved‐caspase 3, Bcl‐2, and Bax as well as p38 were detected. Results showed that lycopene alleviated the liver pathological damage and reduced the injury to liver function induced by nano‐TiO2, as well as decreased nano‐TiO2‐induced ROS. Meanwhile, lycopene mitigated apoptosis resulting from nano‐TiO2, accompanied by the reversed expression of apoptosis‐related proteins. Furthermore, lycopene significantly reversed the upregulation of p‐p38 induced by nano‐TiO2. In conclusion, this study demonstrated that nano‐TiO2 resulted in hepatocyte apoptosis through ROS/ROS‐p38 MAPK pathway and led to liver function injury. Lycopene protected mice liver against the hepatotoxicity of nano‐TiO2 through antioxidant property.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Protective effects of lycopene on TiO₂ nanoparticle‐induced damage in the liver of mice

Chang

Deng

et al. 2023

J of Applied Toxicology

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“…Previous study on titanium dioxide and benzopyrene-induced toxicity reported that its testicular toxicity was treated by lycopene through reduction of oxidative stress and suppressing the apoptosis to protect germinal cells [ 42 , 43 ]. Furthermore, lycopene has demonstrated the anti-infertility effect against drug-induced testicular damage caused by gentamicin, cisplatin, and deltamethrin mainly through the reduction of lipid peroxidation and oxidative stress and increased production of testosterone [ 44 ].…”

Section: Discussionmentioning

confidence: 99%

Comparative Potential of Zinc Sulfate, L-Carnitine, Lycopene, and Coenzyme Q10 on Cadmium-Induced Male Infertility

Iftikhar

Akhtar

Saleem

et al. 2022

International Journal of Endocrinology

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The human exposure to toxic chemicals and heavy metals is one of the main predisposing factors contributing to male infertility. Acute exposure to cadmium chloride results in testicular damage and infertility. The purpose of the present study was to investigate and compare the curative effect of coenzyme Q10 (CoQ10), lycopene, L-carnitine (LC), and zinc sulfate against the cadmium-induced infertility in male Wistar rats. Cadmium chloride (0.4 mg/kg/day) was orally administered to rats for three consecutive days. Then, oral administration of different treatments (i.e., LC 100 mg/kg, CoQ10 20 mg/kg, lycopene 4 mg/kg, zinc sulfate 6 mg/kg, and a combination LC-CoQ10 at 500/50 mg/kg) was carried out for 30 days. The impact of different treatments on semen parameters, such as sperm count and motility, testicular antioxidants, and serum testosterone, was determined. Furthermore, the morphology of epididymis sperms and histopathology of rat testes were also assessed. Cadmium exposure decreased the sperm count, progressive sperm motility, testosterone, superoxide dismutase (SOD), and catalase and reduced glutathione (GSH). It also caused banana sperm tail, bent sperm head, vacuolization of seminiferous tubules, and oligospermia in rat testes. All treatments with nutraceuticals improved sperm count, sperm morphology, serum testosterone, vacuolization of seminiferous tubules, and oligospermia in diseased rats. Treatment with lycopene, LC, and LC-CoQ10 improved progressive sperm motility and other parameters and increased SOD, GSH, and CAT in the rat testes. CoQ10 also increased SOD activity in rat testes’ tissue homogenates. It is concluded from the current study that all nutraceuticals partially improved reproductive toxicity of cadmium. The administration of lycopene and a high-dose combination of LC-CoQ10 were more efficacious in treating cadmium-induced infertility than other treatments. Treatment of cadmium-exposed rats with lycopene, LC, CoQ10, and LC-CoQ10 improved sperm count and motility through reduction of testicular oxidative stress and improving serum testosterone.

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“…Among the most commonly described cytotoxic effects induced by nano-TiO 2 are oxidative stress, DNA damage, together with changes of the cell cycle, which could inhibit cell proliferation and cause apoptosis and necrosis. Cell survival and proliferation are the keys to development and homeostasis and also key events linked to cancer.…”

Section: Introductionmentioning

confidence: 99%

Effect of Titanium Dioxide Nanoparticles on Mammalian Cell Cycle In Vitro: A Systematic Review and Meta-Analysis

Chang

Wang

Meng

et al. 2022

Chem. Res. Toxicol.

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Although most studies that explore the cytotoxicity of titanium dioxide nanoparticles (nano-TiO2) have focused on cell viability and oxidative stress, the cell cycle, a basic process of cell life, can also be affected. However, the results on the effects of nano-TiO2 on mammalian cell cycle are still inconsistent. A systematic review and meta-analysis were therefore performed in this research based on the effects of nano-TiO2 on the mammalian cell cycle in vitro to explore whether nano-TiO2 can induce cell cycle arrest. Meanwhile, the impact of physicochemical properties of nano-TiO2 on the cell cycle in vitro was investigated, and the response of normal cells and cancer cells was compared. A total of 33 articles met the eligibility criteria after screening. We used Review Manager 5.4 and Stata 15.1 for analysis. The results showed an increased percentage of cells in the sub-G1 phase and an upregulation of the p53 gene after being exposed to nano-TiO2. Nevertheless, nano-TiO2 had no effect on cell percentage in other phases of the cell cycle. Furthermore, subgroup analysis revealed that the cell percentage in both the sub-G1 phase of normal cells and S phase of cancer cells were significantly increased under anatase-form nano-TiO2 treatment. Moreover, nano-TiO2 with a particle size <25 nm or exposure duration of nano-TiO2 more than 24 h induced an increased percentage of normal cells in the sub-G1 phase. In addition, the cell cycle of cancer cells was arrested in the S phase no matter if the exposure duration of nano-TiO2 was more than 24 h or the exposure concentration was over 50 μg/mL. In conclusion, this study demonstrated that nano-TiO2 disrupted the cell cycle in vitro. The cell cycle arrest induced by nano-TiO2 varies with cell status and physicochemical properties of nano-TiO2.

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Lycopene Alleviates Titanium Dioxide Nanoparticle-Induced Testicular Toxicity by Inhibiting Oxidative Stress and Apoptosis in Mice

Cited by 23 publications

References 56 publications

Protective effects of lycopene on TiO₂ nanoparticle‐induced damage in the liver of mice

Protective effects of lycopene on TiO₂ nanoparticle‐induced damage in the liver of mice

Comparative Potential of Zinc Sulfate, L-Carnitine, Lycopene, and Coenzyme Q10 on Cadmium-Induced Male Infertility

Effect of Titanium Dioxide Nanoparticles on Mammalian Cell Cycle In Vitro: A Systematic Review and Meta-Analysis

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Lycopene Alleviates Titanium Dioxide Nanoparticle-Induced Testicular Toxicity by Inhibiting Oxidative Stress and Apoptosis in Mice

Cited by 23 publications

References 56 publications

Protective effects of lycopene on TiO2 nanoparticle‐induced damage in the liver of mice

Protective effects of lycopene on TiO2 nanoparticle‐induced damage in the liver of mice

Comparative Potential of Zinc Sulfate, L-Carnitine, Lycopene, and Coenzyme Q10 on Cadmium-Induced Male Infertility

Effect of Titanium Dioxide Nanoparticles on Mammalian Cell Cycle In Vitro: A Systematic Review and Meta-Analysis

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Protective effects of lycopene on TiO₂ nanoparticle‐induced damage in the liver of mice

Protective effects of lycopene on TiO₂ nanoparticle‐induced damage in the liver of mice