Lycopene Estimation in Tomato Lines Using Infrared Absorbance and Tomato Analyzer

Panthee, Dilip R.; Perkins-Veazie, P.; Randall, Dan; Brown, Allan

doi:10.1080/19315260.2012.715324

Cited by 11 publications

(10 citation statements)

References 30 publications

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“…The crimson gene has been reported to increase the level of lycopene in tomato by up to 74% [30]. However, in our previous study, it was not proved to be true [31]. In the present study, a clear association between fruit color and lycopene level was established, which was not new information.…”

Section: Discussionmentioning

confidence: 39%

Diallel Analysis for Lycopene Content in the Hybrids Derived from Different Colored Parents in Tomato

Panthee

Perkins‐Veazie²,

Anderson³

et al. 2015

AJPS

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Lycopene is a red pigment in tomato with purported antioxidant properties. As the amount of lycopene has been reported to differ in different colored tomatoes or even absent in non-red tomatoes, the objective of this study was to investigate the inheritance of lycopene content and the color parameters of hybrids using a 10 parent diallel excluding reciprocals. Parents differed in general combining ability (GCA) for lycopene and color parameters. "Chocolate Stripe" (purple and brown striped) had the highest lycopene content followed by NC 1CS (red colored with the crimson gene) with the best general combining ability among parental lines. Hybrids differed in lycopene content and color parameters across two summer and greenhouse experiments, with no interaction between experiments indicating that the lycopene content was consistent. Narrow-sense heritability for lycopene was only 9% whereas broad-sense heritability was estimated to be 25%. The order of dominance for lycopene content was found as purple-brown > red > blue > yellow > green. Heritability estimates for color parameters were close to those for lycopene. This information may be useful in developing specialty type tomatoes or increasing lycopene content in tomatoes.

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Section: Discussionmentioning

confidence: 39%

Diallel Analysis for Lycopene Content in the Hybrids Derived from Different Colored Parents in Tomato

Panthee

Perkins‐Veazie²,

Anderson³

et al. 2015

AJPS

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“…39 Honest et al 31 reviewed the literature on the relationship between lycopene isomerization and bioavailability and bioactivity properties. Additional studies designed to optimize the analysis of lycopene and its isomers in different milieus are described by Panthee et al 13 and Luterotti et al 40 The availability of C-13 labeled lycopene is expected to facilitate human bioavailability, metabolism, and pharmacokinetic studies. 41,42 Content in Fresh Tomatoes.…”

Section: ■ Lycopenementioning

confidence: 99%

“…Tomato plants (Lycopersicon esculentum) synthesize the bioactive carotenoid pigments lycopene and β-carotene and the glycoalkaloids dehydrotomatine, α-tomatine, and esculeoside A, possibly as a defense against bacteria, fungi, viruses, and insects. About 20 years ago, we initiated studies designed to increase our knowledge of the analysis, composition, and bioactivities of tomato compounds, cited here in chronological order. − …”

Section: Introductionmentioning

confidence: 99%

Anticarcinogenic, Cardioprotective, and Other Health Benefits of Tomato Compounds Lycopene, α-Tomatine, and Tomatidine in Pure Form and in Fresh and Processed Tomatoes

Friedman¹

2013

J. Agric. Food Chem.

220

157

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Tomatoes produce the bioactive compounds lycopene and α-tomatine that are reported to have potential health-promoting effects in animals and humans, but our understanding of the roles of these compounds in the diet is incomplete. Our current knowledge gained from the chemistry and analysis of these compounds in fresh and processed tomatoes and from studies on their bioavailability, bioactivity, and mechanisms of action against cancer cells and other beneficial bioactivities including antibiotic, anti-inflammatory, antioxidative, cardiovascular, and immunostimulating effects in cells, animals, and humans is discussed and interpreted here. Areas for future research are also suggested. The collated information and suggested research might contribute to a better understanding of the agronomical, biochemical, chemical, physiological, molecular, and cellular bases of the health-promoting effects and facilitate and guide further studies needed to optimize the use of lycopene and α-tomatine in pure form and in fresh tomatoes and processed tomato products to help prevent or treat human disease.

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“…Since the tangerine mutation resulted in a non-functional crtiso in NC 1Y [ 17 , 44 ], we compared the relative expression levels of these upstream genes in HLYs as a group with that of crtiso in NC 1Y at both stages (0.0177 ± 0.0129 and 0.0058 ± 0.0033, respectively). We found that most of these upstream genes at either stage as a group had significantly higher relative expression levels than that of crtiso in NC 1Y ( Table S1 ).…”

Section: Resultsmentioning

confidence: 99%

“…Moneymaker is a red tomato line and has wild-type alleles for hp-2 j / hp-2 j . NC 1Y has the tangerine mutation in the encoding region of CrtISO and produces a significant amount of prolycopene at the expense of lycopene synthesis [ 17 , 44 ].…”

Section: Methodsmentioning

confidence: 99%

Coordinated transcriptional regulation of the carotenoid biosynthesis contributes to fruit lycopene content in high-lycopene tomato genotypes

Duduit

Kosentka

Miller

et al. 2022

Horticulture Research

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Lycopene content in tomato fruit is largely under genetic control and varies greatly among genotypes. Continued improvement of lycopene content in elite varieties with conventional breeding has become challenging, in part because little is known about the underlying molecular mechanisms in high-lycopene tomatoes (HLYs). We collected 42 HLYs with different genetic backgrounds worldwide. High-performance liquid chromatography (HPLC) analysis revealed lycopene contents differed among the positive control wild tomato Solanum pimpinellifolium, HLYs, the normal lycopene cultivar “Moneymaker”, and the non-lycopene cultivar NC 1Y at the pink and red ripe stages. Real-time RT-PCR analysis of expression of the 25 carotenoid biosynthesis pathway genes of each genotype showed a significantly higher expression in nine upstream genes (GGPPS1, GGPPS2, GGPPS3, TPT1, SSU II, PSY2, ZDS, CrtISO and CrtISO-L1 but not the well-studied PSY1, PDS and Z-ISO) at the breaker and/or red ripe stages in HLYs compared to Moneymaker, indicating a higher metabolic flux flow into carotenoid biosynthesis pathway in HLYs. Further conversion of lycopene to carotenes may be prevented via the two downstream genes (β-LCY2 and ε-LCY), which had low-abundance transcripts at either or both stages. Additionally, the significantly higher expression of four downstream genes (BCH1, ZEP, VDE, and CYP97C11) at either or both ripeness stages leads to significantly lower fruit lycopene content in HLYs than in the wild tomato. This is the first systematic investigation of the role of the complete pathway genes in regulating fruit lycopene biosynthesis across many HLYs, and enables tomato breeding and gene editing for increased fruit lycopene content.

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Lycopene Estimation in Tomato Lines Using Infrared Absorbance and Tomato Analyzer

Cited by 11 publications

References 30 publications

Diallel Analysis for Lycopene Content in the Hybrids Derived from Different Colored Parents in Tomato

Diallel Analysis for Lycopene Content in the Hybrids Derived from Different Colored Parents in Tomato

Anticarcinogenic, Cardioprotective, and Other Health Benefits of Tomato Compounds Lycopene, α-Tomatine, and Tomatidine in Pure Form and in Fresh and Processed Tomatoes

Coordinated transcriptional regulation of the carotenoid biosynthesis contributes to fruit lycopene content in high-lycopene tomato genotypes

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