Lymphocyte DNA Damage in Chewing Tobacco Users of Coimbatore, Tamilnadu, by Using Comet Assay

Pappuswamy, Manikantan; Balachandar, V.; Sasikala, K.; Mohanadevi, S.

doi:10.1080/09709274.2010.11906297

Cited by 8 publications

(5 citation statements)

References 29 publications

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“…Tail moment is a measure of the DNA damage, which is obtained by combining the amount of DNA in the tail with a distance of migration. The MTM observed in our study, was greater in OLP and OLR when compared to controls which is similar to the higher MTM values observed among smokers in different studies[ 18 19 20 ] [ Table 7 ].…”

Section: Discussionsupporting

confidence: 91%

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A case-control study to detect the extent of DNA damage in oral lichen planus and oral lichenoid reactions using comet assay

Rangdhol

Sitra

et al. 2015

J Pharm Bioall Sci

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Aim:This study aims to quantify the extent of DNA damage in lymphocytes of patients with oral lichen planus (OLP) and oral lichenoid reactions (OLRs) using comet assay.Methodology:Lymphocytes from peripheral blood were subjected to alkaline comet assay. Comet length (CL), head diameter (HD), percentage of DNA in head, tail length (TL), percentage of DNA in tail, tail intensity, tail mean and tail moment were compared between study group (OLP and OLR) and control group using Student's t-test. Pearson's correlation coefficient was used to examine the linear association between the variables.Results:Significantly higher levels of DNA damage was present in study group as reflected by CL, HD and TL, tail intensity and tail moment with P = 0.0001; percentage of DNA in head and tail with P = 0.02 and tail mean with P = 0.012.Conclusion:This study brings out the fact that DNA damage measured by comet assay was greater in the study group when compared to the control group. As a reflection of uniqueness, this study crowns the scenario with respect to early detection and prevention of potentially malignant disorders and the process of malignant transformation.

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Section: Discussionsupporting

confidence: 91%

“…This study was done to detect the extent of DNA damage in OLP and OLRs using comet assay. [ 15 16 17 18 19 20 ]…”

Section: Comet Assay In Detecting Dna Damagementioning

confidence: 99%

A case-control study to detect the extent of DNA damage in oral lichen planus and oral lichenoid reactions using comet assay

Rangdhol

Sitra

et al. 2015

J Pharm Bioall Sci

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“…In addition to oral cancer and leukoplakia, we also observed increased level of serum b2-microglobulin in patients exposed to various carcinogens with no oral lesions (Group III). There are no studies carried out to estimate the b2-microglobulin levels in these individuals so far, but there are studies carried out to evaluate the lymphocyte DNA damage in individuals exposed to carcinogens which suggested that tobacco can induce lymphocyte DNA damage and there is a synergistic effect of carcinogens on DNA damage [16][17][18]. During the course of tumor development, quantitative changes have been shown to occur in a variety of substances in serum, such as various biochemical or tumor markers.…”

Section: Discussionmentioning

confidence: 99%

Estimation of Serum Beta 2-Microglobulin Levels in Individuals Exposed to Carcinogens: Clinical Study in Indian Population

Saddiwal

Hebbale

Sane

et al. 2016

J. Maxillofac. Oral Surg.

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Aim The aim of the study is to evaluate the prognostic value of b2-m as a biochemical parameter for the diagnosis and prognosis of oral squamous cell carcinoma (SCC). Materials and Methods The study included 60 patients (15 oral SCC, 15 leukoplakia, 15 individuals exposed to various carcinogens and without oral cancerous or precancerous lesions, 15 healthy individuals). The levels of b2-m were estimated using chemi-luminescent immunometric assay on Immulite fully automated machine. Statistical analysis of the data was done using Tuckey's test, ANOVA and independent sample t test. Results Results showed that b2-m was increased in individuals exposed to carcinogens without precancerous and cancerous lesion. Conclusion Serum b2-m can be used as a better indicator and can give an early indication of malignant change and therefore malignancy can be detected at an early and treatable stage.

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“…Thus, numerical deviations of chromosome X have been reported to affect carcinogenesis and poor outcome of different tumor types [50]. One X-chromosome increase has been reported to be relatively common with other karyotypic changes in leukemia, lymphoma, and prostate cancer [51,52]. In addition, Y chromosome loss has been reported to be common in the cancer cells and several leukemia, papillary renal cell carcinoma, prostate cancer, male chest carcinoma, and pancreatic adenocarcinoma.…”

Section: Dıscussıonmentioning

confidence: 99%

The Genotoxic Effect of Interventional Cardiac Radiologic Procedures on Human Chromosomes

Demırhan¹

2020

CMRR

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In recent years, an important part of the ionizing radiation (IR) that human have been exposed for diagnostic purposes are interventional radiologic procedures. The X-rays and contrast media are used in angiography. The patients and staff members are exposed to X-ray during these procedures. While it is known that X-rays cause DNA damage and carcinogenesis, the effect of the contrast agent is still unknown. The aim of this study was to investigate the effect of X-rays and contrast agent on chromosomes of human patients. Peripheral blood samples were taken from 50 patients (30 males, 20 females, ages between 38-75 years). Chromosome analysis of peripheral lymphocytes in 50 patients were performed at 3 different periods; before the interventional radiologic procedure, 24 hours and 1-3 months after the procedure. Also, chromosome analysis was performed on 17 staff members working during interventional radiological procedures to investigate the effect of X-rays. Standard cytogenetic analysis techniques were used for this study. The frequency of chromosomal aberrations (CAs) was significantly higher in patients 24 hours after the interventional radiologic procedures than pretreatment (p=0,000). At the same time, CAs after 24 hours compared with those taken 1-3 months later, shown that the CAs were significantly reduced after 1-3 months (p=0,000). We also found that the frequency of CAs was also statistically higher in patients exposed to high radiation doses (p=0,042). Compared with the control group (n=30), CAs were found significantly higher in workers exposed to radiation. Our findings have shown that X-rays and contrast agents that used in interventional radiological cause chromosomal damage. For this reason, the dose of radiation to be given to the patient must be carefully selected. Due to the potentially high genetic damage of patients with coronary artery disease (CAD), the type and amount of medication to be given and the frequency of radiological diagnostic procedures to be performed should be meticulously adjusted.

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Lymphocyte DNA Damage in Chewing Tobacco Users of Coimbatore, Tamilnadu, by Using Comet Assay

Cited by 8 publications

References 29 publications

A case-control study to detect the extent of DNA damage in oral lichen planus and oral lichenoid reactions using comet assay

A case-control study to detect the extent of DNA damage in oral lichen planus and oral lichenoid reactions using comet assay

Estimation of Serum Beta 2-Microglobulin Levels in Individuals Exposed to Carcinogens: Clinical Study in Indian Population

The Genotoxic Effect of Interventional Cardiac Radiologic Procedures on Human Chromosomes

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