Lymphocyte reactivity during spaceflight

Cogoli, Augusto; Tschopp, Alexander

doi:10.1016/0167-5699(85)90151-3

Cited by 76 publications

(26 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Parabolic flight induces an increase in PMN number and the capability of PMNs to produce hydrogen peroxide (H 2 O 2 ) in response to soluble stimuli (Kaufmann et al 2009). These works seemingly indicate that the action of microgravity has enhanced the immune response of PMNs, which is inconsistent with the reduced capability of lymphocytes (Cogoli and Tschopp 1985;Konstantinova et al 1993;Meshkov and Rykova 1995;Mills et al 2002;Mills et al 2008Mills et al , 2013, monocytes (Kaur et al 2005(Kaur et al , 2008Crucian et al 2011), NK cells (Rykova et al 1992;Konstantinova et al 1993Konstantinova et al , 1995Mehta et al 2001), and macrophages (Armstrong et al 1995;Hsieh et al 2005;Ortega et al 2012;Adrian et al 2013;Wang et al 2014Wang et al , 2015Brungs et al 2015;Paulsen et al 2015) under microgravity. The underlying mechanisms for such the difference remain unclear.…”

Section: Introductionmentioning

confidence: 85%

Effects of Simulated Microgravity on Functions of Neutrophil-like HL-60 Cells

Wang

Zhang

et al. 2015

Microgravity Sci. Technol.

View full text Add to dashboard Cite

Altered gravity, especially microgravity affects cellular functions of immune cells and can result in immune dysfunction for long-term, manned spaceflight and space exploration. The underlying mechanism, however, of sensing and responding to the gravity alteration is poorly understood. Here, a rotary cell culture system (RCCS) bioreactor was used to elucidate the effects of simulated microgravity on polymorphonuclear neutrophils (PMN)-like HL-60 cells. Alteration of cell morphology, up-regulation of (nitric oxide) NO production, enhancement of interleukin-6 (IL-6), interleukin-8 (IL-8), and monocyte chemotactic protein 1 (MCP-1) secretion, and diversity of cellular adhesion molecule expression were observed for the cells cultured in RCCS, leading to the up-regulated inflammatory immune responses and host defense. It was also indicated that such alterations in biological responses of PMNs mediated the reduced rolling velocity and decreased adhesion of PMNlike HL-60 cells on endothelial cells under shear flow. This work furthers the understandings in the effects and mechanism of microgravity on PMN functions, which are Chengzhi Wang and Ning Li, both authors contributed equally to this work. Electronic supplementary material

show abstract

Section: Introductionmentioning

confidence: 85%

Effects of Simulated Microgravity on Functions of Neutrophil-like HL-60 Cells

Wang

Zhang

et al. 2015

Microgravity Sci. Technol.

View full text Add to dashboard Cite

show abstract

“…Immunological studies performed postflight on astronauts and cosmonauts have consistently shown alterations in the numbers and proportions of circulating leukocytes [4] as well as depressed in vitro mitogen-induced T cell activation [5][6][7]. In addition, reduced delayed-type hypersensitivity to a panel of recall antigens during short duration (4-to 10-day) space missions was reported by Taylor and Janney [8].…”

Section: Introductionmentioning

confidence: 99%

Leukocyte subsets and neutrophil function after short-term spaceflight

Stowe

Sams

Mehta³

et al. 1999

Journal of Leukocyte Biology

137

102

View full text Add to dashboard Cite

Changes in leukocyte subpopulations and function after spaceflight have been observed but the mechanisms underlying these changes are not well defined. This study investigated the effects of short-term spaceflight (8-15 days) on circulating leukocyte subsets, stress hormones, immunoglobulin levels, and neutrophil function. At landing, a 1.5-fold increase in neutrophils was observed compared with preflight values; lymphocytes were slightly decreased, whereas the results were variable for monocytes. No significant changes were observed in plasma levels of immunoglobulins, cortisol, or adrenocorticotropic hormone. In contrast, urinary epinephrine, norepinephrine, and cortisol were significantly elevated at landing. Band neutrophils were observed in 9 of 16 astronauts. Neutrophil chemotactic assays showed a 10-fold decrease in the optimal dose response after landing. Neutrophil adhesion to endothelial cells was increased both before and after spaceflight. At landing, the expression of MAC-1 was significantly decreased while L-selectin was significantly increased. These functional alterations may be of clinical significance on long-duration space missions. J. Leukoc. Biol. 65: 179-186; 1999.

show abstract

“…Immunological studies performed after spaceflight have repeatedly shown decreases in T-lymphocyte mitogen stimulation, altered cytokine production, and altered distribution of leukocyte and lymphocyte subsets in peripheral blood [1][2][3][4][5][6]. Relatively few but important inflight studies demonstrating decreased delayed-type hypersensitivity have underscored the need to determine the extent of immune suppression [2,7].…”

Section: Introductionmentioning

confidence: 99%

Stress-Induced Reactivation of Epstein-Barr Virus in Astronauts

Stowe

Pierson

Feeback

et al. 2000

Neuroimmunomodulation

View full text Add to dashboard Cite

Herpesviruses are leading causes of infectious blindness and death in immunocompromised individuals. Impaired cellular immunity, which is known to result in increased frequency and severity of herpesvirus infections, has been demonstrated both during and after spaceflight. Therefore, we examined whether Epstein-Barr virus (EBV), a well-characterized latent herpesvirus, undergoes reactivation in astronauts. Sera from Shuttle astronauts, taken before and after spaceflight, were examined for evidence of EBV reactivation. The geometric mean antibody titer to EBV viral capsid antigen (VCA) was significantly increased prior to flight compared to baseline (p = 0.0001). After spaceflight, evidence of acute lytic replication was found in which 8- to 64-fold increases in EBV early antigen (EA) antibodies occurred without significant increases in antibodies to measles virus. Additionally, stress-induced shifts in circulating leukocytes and elevated levels of urinary cortisol and epinephrine were found. Overall, significant increases in EA or high VCA/EA antibody titers were found in 8 of 23 (35%) male astronauts and 3 of 5 (60%) female astronauts. These results indicate that stress reactivates EBV prior to flight and suggest that acute lytic replication of EBV occurs during spaceflight.

show abstract

Lymphocyte reactivity during spaceflight

Cited by 76 publications

References 7 publications

Effects of Simulated Microgravity on Functions of Neutrophil-like HL-60 Cells

Effects of Simulated Microgravity on Functions of Neutrophil-like HL-60 Cells

Leukocyte subsets and neutrophil function after short-term spaceflight

Stress-Induced Reactivation of Epstein-Barr Virus in Astronauts

Contact Info

Product

Resources

About