Lymphocytes with multiple chromosomal damages in a large cohort of West Siberia residents: Results of long-term monitoring

Дружинин, В. Г.; Баканова, М. Л.; Fučić, Aleksandra; Головина, Т.А.; Savchenko, Yana; Sinitsky, M. Yu.; Volobaev, Valentin P.

doi:10.1016/j.mrfmmm.2015.12.003

Cited by 6 publications

(4 citation statements)

References 59 publications

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“…Interestingly, Table 1 shows that lab DCA_3 also observed a multiaberrant cell, with two dicentrics, in the control sample taken from the sample individual. 'Rogue cells' are observed in both exposed and unexposed populations and there are several potential causes (Rozgaj et al 2002(Rozgaj et al , M'kacher et al 2010Druzhinin et al 2016). The history of this volunteer was reassessed in response to this observation but no recent radiation exposures (X-ray, CT or otherwise) or other potential contributing factors were identified.…”

Section: The Aims Of This Inter-comparison Werementioning

confidence: 96%

Integration of new biological and physical retrospective dosimetry methods into EU emergency response plans – joint RENEB and EURADOS inter-laboratory comparisons

Ainsbury

Badie

Barnard

et al. 2016

International Journal of Radiation Biology

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Purpose: RENEB, 'Realising the European Network of Biodosimetry and Physical Retrospective Dosimetry,' is a network for research and emergency response mutual assistance in biodosimetry within the EU. Within this extremely active network, a number of new dosimetry methods have recently been proposed or developed. There is a requirement to test and/or validate these candidate techniques and inter-comparison exercises are a well-established method for such validation. Materials and methods: The authors present details of inter-comparisons of four such new methods: dicentric chromosome analysis including telomere and centromere staining; the gene expression assay carried out in whole blood; Raman spectroscopy on blood lymphocytes, and detection of radiationinduced thermoluminescent signals in glass screens taken from mobile phones. Results: In general the results show good agreement between the laboratories and methods within the expected levels of uncertainty, and thus demonstrate that there is a lot of potential for each of the candidate techniques. INTERNATIONAL JOURNAL OF RADIATION BIOLOGY, 2017 VOL. 93, NO. 1, 99-109 http://dx.doi.org/10.1080/09553002.2016 Conclusions: Further work is required before the new methods can be included within the suite of reliable dosimetry methods for use by RENEB partners and others in routine and emergency response scenarios.

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Section: The Aims Of This Inter-comparison Werementioning

confidence: 96%

Integration of new biological and physical retrospective dosimetry methods into EU emergency response plans – joint RENEB and EURADOS inter-laboratory comparisons

Ainsbury

Badie

Barnard

et al. 2016

International Journal of Radiation Biology

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“…The correlation between the incidence of rogue cells and the poor prognosis of these populations was demonstrated (Lazutka, ; M'Kacher et al, ). Recently, the presence of rogue cells was reported in subjects exposed to radon and its decay daughter products (Druzhinin et al, ). The presence of rogue cells after paraben exposure requires further investigation.…”

Section: Discussionmentioning

confidence: 99%

Genotoxic risk of ethyl-paraben could be related to telomere shortening

Finot¹,

Kaddour

Morat

et al. 2016

J. Appl. Toxicol.

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The ability of parabens to promote the appearance of multiple cancer hallmarks in breast epithelium cells provides grounds for regulatory review of the implication of the presence of parabens in human breast tissue. It is well documented that telomere dysfunction plays a significant role in the initiation of genomic instability during carcinogenesis in human breast cancer. In the present study, we evaluated the genotoxic effect of ethyl 4-hydroxybenzoate (ethyl-paraben), with and without metabolic activation (S9), in studies following OECD guidelines. We observed a significant increase in genotoxic damage using the Mouse Lymphoma Assay and in vitro micronucleus (MN) tests in the L5178Y cell line in the presence of S9 only after a short exposure. A high frequency of MN was observed in the TK6 cells after a short exposure (3 h) in the presence of S9 and a long exposure (26 h) without S9. We found significant increases in the MN frequency and induced chromosomal aberrations in the lymphocytes of only one donor after ethyl-paraben exposure in the presence of S9 after a short exposure. Cytogenetic characterization of the paraben-treated cells demonstrated telomere shortening associated with telomere loss and telomere deletions in L5178Y and TK6 cells and lymphocytes of the paraben sensitive-donor. In a control cohort of 68 human lymphocytes, telomere length and telomere aberrations were age-dependent and showed high inter-individual variation. This study is the first to link telomere shortening and the genotoxic effect of ethyl paraben in the presence of S9 and raises the possibility that telomere shortening may be a proxy for underlying inter-individual sensitivity to ethyl-paraben. Copyright © 2016 John Wiley & Sons, Ltd.

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“…Recent publications on rogue cells in different populations strongly suggest that they are biomarker of internal exposure to high LET radiation, primarily by alpha particles, incorporated inside the body (Popova et al 2004;Aseeva et al 2010;Druzhinin et al 2016). Rogue cells were reported in cancer patients for different cancer types, both in children and adults, showing that such cells may be specific biomarkers of cancerogenesis (Minina et al 2016;Leonard et al 1995).…”

Section: Children Living In Territories Contaminated By Radionuclidesmentioning

confidence: 99%

Follow-up studies on genome damage in children after Chernobyl nuclear power plant accident

et al. 2016

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As children are more susceptible to ionizing radiation than adults, each nuclear accident demands special attention and care of this vulnerable population. The Chernobyl nuclear disaster occurred in a region populated with a large number of children, but despite all efforts and expertise of nuclear specialists, it was not possible to avoid casualties. As vast regions of Ukraine, Belarus and Russia were exposed to doses of ionizing radiation, which are known to be related with different diseases, shortly after the accident medical surveillance was launched, which also included analysis of genome damage. Child population affected by internal and external radiation consisted of subjects exposed prenatally, postnatally (both evacuated and non-evacuated), born by irradiated fathers who worked as liquidators, and parents exposed environmentally. In all groups of children during the last 30 years who were exposed to doses which were significantly higher than that recommended for general population of 1 mSv per year, increased genome damage was detected. Increased genome damage includes statistically higher frequency of dicentric and ring chromosomes, chromated and chromosome breaks, acentric fragments, translocations, and micronuclei. The presence of rogue cells confirmed internal contamination. Genome instability and radiosensitivity in children was detected both in evacuated and continuously exposed children. Today the population exposed to ionizing radiation in 1986 is in reproductive period of life and follow-up of this population and their offspring is of great importance. This review aims to give insight in results of studies, which reported genome damage in children in journals without language restrictions.

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Lymphocytes with multiple chromosomal damages in a large cohort of West Siberia residents: Results of long-term monitoring

Cited by 6 publications

References 59 publications

Integration of new biological and physical retrospective dosimetry methods into EU emergency response plans – joint RENEB and EURADOS inter-laboratory comparisons

Integration of new biological and physical retrospective dosimetry methods into EU emergency response plans – joint RENEB and EURADOS inter-laboratory comparisons

Genotoxic risk of ethyl-paraben could be related to telomere shortening

Follow-up studies on genome damage in children after Chernobyl nuclear power plant accident

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