Lymphocytic Clusters in Peripheral Blood: An Atypical Morphologic Pattern of Chronic Lymphocytic Leukemia

Abstract

A case of chronic lymphocytic leukemia is described in which peripheral blood and bone marrow films showed lymphocytes arranged in aggregates resembling epithelial cell clusters. Due to this morphological conformation, automatic hemogram screening was not able to detect the presence of chronic lymphocytic leukemia. Correct interpretation of a typical histogram pattern led to the final diagnosis.

Regulation of leukemic cell adhesion, proliferation, and survival by β-catenin

“…Adhesion of leukemic cells to each other occurs in vitro, both spontaneously and in response to mitogens and certain drugs, and in vivo, in certain pathological states. 39,40 We triggered homotypic cell adhesion with PHA in Jurkat cells transiently transfected with expression vectors including wild-type ␤-catenin or full-length ␤-catenin antisense. The PHA-mediated homotypic adhesion of Jurkat cells, which occurs very rapidly and was analyzed after a 30-minute exposure, was increased by expression of wild-type ␤-catenin and, conversely, decreased by expression of full-length ␤-catenin antisense (Figure 4).…”

“…Leukemic cells undergo homotypic adhesion in response to various stimuli both in vitro and in vivo. 39,40 To analyze whether ␤-catenin is involved in homotypic aggregation after Jurkat cell activation, cells were transfected with control constructs or constructs encoding full-length ␤-catenin sense or antisense and cotransfected with a construct encoding GFP. After flow cytometric isolation of GFP-positive cells, the transfected cells were treated with PHA, 38 and the effect of ␤-catenin antisense was determined.…”

Caso de linfoma No Hodgkin detectado por aglutinación de linfocitos en sangre periférica

“…consecuentemente podría ser considerado como un hallazgo precoz de alerta sobre la presencia de linfocitos neoplásicos en sangre periférica, en especial en pacientes adultos mayores donde este tipo de patologías es más frecuente [7][8][9].…”

“…Este hallazgo y su asociación a linfomas ya ha sido informado en varias publicaciones previas lo que eventualmente, podría ser considerado como una manifestación precoz que podría alertar sobre la posible presencia de linfocitos neoplásicos en sangre periférica, en especial si se trata de pacientes adultos de edad avanzada donde este ogías es más frecuente [7][8][9].…”

Bulky plasmacytoma of the skull with intracranial involvement

“…However, we previously reported normal plasma fibrinogen, Plasma ACTH (pg/ml) 5 6 9-52 antithrombin III, protein C, tissue plasminogen activator [2], thrombin/ Serum interleukin-6 (pg/ml) Ͼ1,000 405 Ͻ4.0 antithrombin III complex (TAT), and prothrombin fragment 1.2 (F1.2) [3], but lower D-dimer and higher plasminogen activator inhibitor (PAI) [2] *CRP, C-reactive protein; ACTH, adrenocorticotropin. bly related to abnormalities in the fibrinolytic system due to lung damage 6. thrombin as can be bound with bovine protein Z.…”