Lymphoid stem cell identification in the developing thymus and bursa of fabricius of the chick

Leene, W.; Duyzings, M. J. M.; Steeg, C. van

doi:10.1007/bf00307368

Cited by 68 publications

(24 citation statements)

References 22 publications

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“…The CAM has long been a favored system for the study of tumor angiogenesis and metastasis (Dagg et al, 1956;Auerbach et al, 1976;Ribatti et al, 1996;Quigley and Armstrong, 1998), because at this stage the chick's immunocompetence system is not fully developed and the conditions for rejection have not been established (Leene et al, 1973). In fact, immunocompetence in birds develops only after hatching (Weber and Mausner, 1977).…”

Section: Employment Of the Cam In The Study Of Tumor Angiogenesis Andmentioning

confidence: 99%

“…Investigation of histological CAM sections would help to detect the presence of a perivascular inflammatory infiltrate, together with a hyperplastic reaction, if any, of the chorionic epithelium. However, a nonspecific inflammatory response is much less likely when the test material is grafted as soon as the CAM begins to develop, while the host's immune system is relatively immature (Leene et al, 1973). There are two other drawbacks to the CAM assay.…”

Section: Limitation Of the Cam Assaymentioning

confidence: 99%

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Chorioallantoic membrane capillary bed: A useful target for studying angiogenesis and anti‐angiogenesis in vivo

et al. 2001

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The chick embryo chorioallantoic membrane (CAM) is an extraembryonic membrane that is commonly used in vivo to study both angiogenesis and anti-angiogenesis. This review 1) summarizes the current knowledge about the structure of the CAM's capillary bed; 2) discusses the controversy about the existence of a single blood sinus or a capillary plexus underlying the chorionic epithelium; 3) describes a new model of the CAM vascular growth, namely the intussusceptive mode; 4) reports findings regarding the role played by endogenous fibroblast growth factor-2 in CAM vascularization; and 5) addresses the use and limitations of the CAM as a model for studying angiogenesis and anti-angiogenesis. Anat Rec 264: [317][318][319][320][321][322][323][324] 2001.

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Section: Employment Of the Cam In The Study Of Tumor Angiogenesis Andmentioning

confidence: 99%

Section: Limitation Of the Cam Assaymentioning

confidence: 99%

Chorioallantoic membrane capillary bed: A useful target for studying angiogenesis and anti‐angiogenesis in vivo

et al. 2001

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“…Surgical sponges are also used as in vivo immune arenas for studying the kinetics, sequence, and magnitude of leukocyte infiltration into sites of immune activation (13,14). They can be used to locally administer chemoattractants and retain the resulting cellular infiltrates for further analyses (15,16). To test our first hypothesis, we examined the effects of acute stress on neutrophil, monocyte, natural killer (NK) cell, and T cell trafficking into a surgical sponge at 6, 24, 48, and 72 h after implantation.…”

mentioning

confidence: 99%

Stress-induced enhancement of leukocyte trafficking into sites of surgery or immune activation

Viswanathan

Dhabhar

2005

Proc. Natl. Acad. Sci. U.S.A.

215

161

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Effective immunoprotection requires rapid recruitment of leukocytes into sites of surgery, wounding, infection, or vaccination. In contrast to immunosuppressive chronic stressors, short-term acute stressors have immunoenhancing effects. Here, we quantify leukocyte infiltration within a surgical sponge to elucidate the kinetics, magnitude, subpopulation, and chemoattractant specificity of an acute stress-induced increase in leukocyte trafficking to a site of immune activation. Mice acutely stressed before sponge implantation showed 200 -300% higher neutrophil, macrophage, natural killer cell, and T cell infiltration than did nonstressed animals. We also quantified the effects of acute stress on lymphotactin-(LTN; a predominantly lymphocyte-specific chemokine), and TNF-␣-(a proinflammatory cytokine) stimulated leukocyte infiltration. An additional stress-induced increase in infiltration was observed for neutrophils, in response to TNF-␣, macrophages, in response to TNF-␣ and LTN, and natural killer cells and T cells in response to LTN. These results show that acute stress initially increases trafficking of all major leukocyte subpopulations to a site of immune activation. Tissue damage-, antigen-, or pathogen-driven chemoattractants subsequently determine which subpopulations are recruited more vigorously. Such stress-induced increases in leukocyte trafficking may enhance immunoprotection during surgery, vaccination, or infection, but may also exacerbate immunopathology during inflammatory (cardiovascular disease or gingivitis) or autoimmune (psoriasis, arthritis, or multiple sclerosis) diseases.chemokine ͉ psychophysiological stress ͉ surgical sponge ͉ wound healing ͉ lymphotactin

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“…CAM is used to investigate tumor angiogenesis and metastasis. However, as the chick's immune system is not completely developed at the early stages of chick embryo formation (12), the CAM assay has limitations including non-specific inflammatory reactions (13) and rearrangement of existing vessels that follows contraction of the membrane (14). These limitations influence the observations of vasoproliferative responses.…”

Section: Introductionmentioning

confidence: 99%

A novel four-step system for screening angiogenesis inhibitors

Zhou

et al. 2013

Molecular Medicine Reports

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Abstract. Angiogenesis exhibits a significant effect on tumor progression. Inhibiting angiogenesis may provide significant advantages over currently available therapeutics for cancer therapies thus, the development of a system of screening angiogenesis is essential. In the present study, a novel available system of screening angiogenesis inhibitors by four steps was developed. The chorioallantoic membrane (CAM), yolk sac membrane and early chick embryo blood island assay were initially performed to obtain possible antitumor compounds. The MMTV-PyMT transgenic breast cancer mouse model was used for final screening and to confirm potential antitumor effects. Four angiogenesis inhibitors were isolated from 480 compounds, which were obtained from ICCB known bioactives library, by a combination of the CAM, yolk sac membrane and early chick embryo blood island assay. The MMTV-PyMT mouse was treated with one of four agents and it was demonstrated that the tumor volume was significantly inhibited. These results demonstrate that the four-step screening system is feasible.

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Lymphoid stem cell identification in the developing thymus and bursa of fabricius of the chick

Cited by 68 publications

References 22 publications

Chorioallantoic membrane capillary bed: A useful target for studying angiogenesis and anti‐angiogenesis in vivo

Chorioallantoic membrane capillary bed: A useful target for studying angiogenesis and anti‐angiogenesis in vivo

Stress-induced enhancement of leukocyte trafficking into sites of surgery or immune activation

A novel four-step system for screening angiogenesis inhibitors

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