Lymphoma cell line (FL-18) and Epstein-Barr virus-carrying cell line (FL-18-EB) obtained from a patient with follicular lymphoma: monoclonal derivation and different properties

Abstract

A novel cell line, FL-18, was established from the pleural effusion of a patient with follicular small cleaved cell lymphoma. At the same time, an Epstein-Barr virus (EBV) nuclear antigen (EBNA)-positive cell line, FL-18-EB, was established from the EBV-infected culture of the same pleural effusion cells. Both cell lines had the same monoclonal surface immunoglobulin (IgG kappa), and they had the same karyotype as that of the fresh pleural effusion cells in which a reciprocal translocation between the long arm of chromosomes 14 and 18 [t(14;18)(q32;q21)] was detected. Gene rearrangement analysis of immunoglobulin heavy-chain gene (JH) and kappa light-chain gene (J kappa) showed the same rearranged configuration in the two cell lines; however, some morphological and phenotypic differences were found. The FL-18-EB cells, which were morphologically similar to common EBNA- positive lymphoblastoid cell lines of normal B cell origin at the initial phase of culture, were larger than the FL-18 cells and contained multinucleated giant cells. The FL-18 cells lacked cytoplasmic immunoglobulin and were positive for common acute lymphoblastic leukemia antigen (CALLA), whereas the FL-18-EB cells had cytoplasmic immunoglobulin and were negative for CALLA. Thus, the phenotype of FL-18-EB seems to be a result of a shift by EBV infection to a more mature stage in the B cell differentiation pathway than that of FL-18. The paired availability of EBV-free and EBV-infected cell lines of a neoplastic clone is unique and valuable in considering EBV infectibility of neoplastic B cells and resultant phenotypic changes.

Establishment and characterization of a novel ‘double‐hit’ follicular lymphoma cell line, FL‐SJC

“…However, as far as we know, there have been only a few published manuscripts describing the establishment and characterization of defined DHFL cell lines 3,13,18‐22 . Some of them are not in full detail in genetic messages 13,18,19 .…”

“…28,29 However, as far as we know, there have been only a few published manuscripts describing the establishment and characterization of defined DHFL cell lines. 3,13,[18][19][20][21][22] Some of them are not in full detail in genetic messages. 13,18,19 In this report, we established a novel 'double-hit' follicular lymphoma cell line, FL-SJC, which we wish as a useful tool for the improved understanding of MYC/BCL2…”

“…3,13,[18][19][20][21][22] Some of them are not in full detail in genetic messages. 13,18,19 In this report, we established a novel 'double-hit' follicular lymphoma cell line, FL-SJC, which we wish as a useful tool for the improved understanding of MYC/BCL2…”

“…Until 2016, nearly 30 cell lines meet the diagnosis of DH lymphoma, bearing both MYC and BCL2 rearrangement. [13][14][15][16][17] Among them, most of them were derived from patients with DLBCL, or Burkitt lymphoma (or B-ALL), while only 4 cell lines were from patients with FL, 13 including FLK-1, 3 FL-18, 18,19 SC-1 20,21 and TAT-1. 22 FLK-1 holding t(2;8)(p12;q24) and t(14;18)(q32;q21), established in 2001, was found to depend on a follicular dendritic cells.…”

“…3 So FLK-1 is unstable and inconvenient as a cell line. 3 FL-18 was established in 1985, in which the translocation [t(8;22)(q24;q13) and t(14;18)(q32;q21)] was not verified by fluorescence in situ hybridization (FISH), Southern blot, polymerase chain reaction (PCR) or other method, 13,18,19 probably due to the previous technical restriction. Only SC1 with t(8;14;18)(q24;q32;q21) 20,21 and TAT-1 22 with t(8;14;18)(q24;q32;q21) had fully documented genetic background.…”