Lysine-Functionalized Tungsten Disulfide Quantum Dots as Artificial Enzyme Mimics for Oxidative Stress Biomarker Sensing

Abstract

The color generating from the biochemical reaction between 3,3′,5,5′-tetramethylbenzidine and Lysine@WS2 QDs was used a signal for the detection of hydrogen peroxide. The QDs were prepared using a combination of techniques, that is, probe sonication and hydrothermal treatment. Analysis via UV–vis spectroscopy, Fourier transform infrared and Raman spectroscopy, X-ray diffraction, energy-dispersive spectroscopy, and transmission electron microscopy yielded detailed information on the nature and characteristics of these quantum dots. Furthermore, as-synthesized quantum dots were studied for their capability to mimic peroxidase enzyme using 3,3′,5,5′-tetramethylbenzidine as a substrate. Consequently, a colorimetric sensor utilizing Lysine@WS2 QDs could detect hydrogen peroxide in a range of 0.1–60 μM with a response time of 5 min. The same material was used for H2O2 detection using impedance spectroscopy, which yielded a dynamic range of 0.1–350 μM with a response time of 30–40 s.

Nanodots Derived from Layered Materials: Synthesis and Applications

“…[38] Up to now, most of the reported QD-based sensors are based on the PL phenomenon while a few are based on other sensing mechanisms such as colorimetric sensing and electrochemical sensing. [195][196][197][198][199]…”

Advancements in 2D Materials Based Biosensors for Oxidative Stress Biomarkers

“…The robustness of the method was checked using urine samples as well. 193 Tungsten disulfide quantum dots were used by Hang et al for the fluorescent detection of hydrogen peroxide and glucose. The presence of hydrogen peroxide resulted in the quenching of WS 2 QDs fluorescence, proportional to the analyte concentration.…”

“…The peroxidase activity of lysine functionalized tungsten disulfide quantum dots was used for the opto-electrochemical detection of hydrogen peroxide by Garg and co-workers. The robustness of the method was checked using urine samples as well …”

On‐Site Detection for Hazardous Materials in Chemical Accidents

“…68 Interestingly, the authors have developed new nanosensor platforms which aimed for H 2 O 2 monitoring as lysinefunctionalized tungsten disulfide quantum dots. 69 Furthermore, gold nanorods were immobilized on a glassy carbon electrode leading to a plasmon-enhanced electrochemical biosensor device for H 2 O 2 sensoring. 70 These nanosensor system is versatile, their methods of differential sensing could be extended to not only apply for H 2 O 2 detection in many fields but also monitor of other hazardous chemical by modifying the chemistry of the sensing nanomaterials.…”

“…Recently, there are many sensor platforms designed for H 2 O 2 detection which satisfied different applications such as Au@MnO 2 @Raman reporter—three‐in‐one stimulus‐responsive nanoplatform, 66 paper‐based wearable sensor, 67 and self‐referenced optical fiber sensor 68 . Interestingly, the authors have developed new nanosensor platforms which aimed for H 2 O 2 monitoring as lysine‐functionalized tungsten disulfide quantum dots 69 . Furthermore, gold nanorods were immobilized on a glassy carbon electrode leading to a plasmon‐enhanced electrochemical biosensor device for H 2 O 2 sensoring 70 .…”