Lysozyme activity determination using peptidoglycan from the cell wall of Micrococcus lysodeikticus

Goncharova, A. M.; Ziamko, V.Y.; Окулич, В. К.

doi:10.14427/jipai.2018.1.48

Cited by 2 publications

(1 citation statement)

References 11 publications

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“…In the third group, the combination preparation Echinacea Compositum S and applications under the bases of prostheses 1 % cream Clotrimazole restored the process of epithelialization. To assess the local immunity of the oral mucosa, the humoral factors of immunity were studied -the concentration of S-IgA and lysozyme [23] (Fig. 6).…”

Section: Results and Their Discussionmentioning

confidence: 99%

Influence of Type 2 Diabetes Mellitus on the Prevention and Treatment of Prosthetic Stomatitis

Badalov

Chernyavskaya

2021

PEP

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Purpose: to study the effect of type 2 diabetes mellitus on the state of the mucous membrane and tissues of the oral cavity to optimize the prevention and increase the effectiveness of dentition defects complex treatment in this contingent of patients. Materials and methods. We examined 112 patients with type 2 diabetes mellitus aged 35 to 64 years, who were further divided into three groups. The state of the oral mucosa, morphological changes, local immunity, lysozyme were determined. The work used morphological, microscopic methods, enzyme immunoassay and spectrophotometry. To test the statistical significance of intergroup differences, the nonparametric Mann- Whitney test was used. Results. Oral use in patients with diabetes mellitus type 2, applications of 1% Clotrimazole cream and Echinacea Compositum S had a positive preventive and therapeutic effect, which was accompanied by an increase in capillary resistance, a decrease in the degree of migration of leukocytes, an increase in the functional activity of immune system cells and an increase in the level of regenerative processes. In patients with defects in the dentition against the background of type 2 diabetes mellitus, violations of the oral cavity local immunity were revealed, characterized by a significant increase in the S-IgA content and a decrease in lysozyme.Orthopedic treatment with partial removable denturesled to a significant decrease in the concentration of S-IgA and an increase in the bactericidal activity of saliva due to increased lysozyme synthesis. It was concluded that type 2 diabetes mellitus affects the state of the oral mucosa, which must be taken into account when providing orthopedic and dental treatment.

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Section: Results and Their Discussionmentioning

confidence: 99%

Influence of Type 2 Diabetes Mellitus on the Prevention and Treatment of Prosthetic Stomatitis

Badalov

Chernyavskaya

2021

PEP

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Influence of new antimicrobial peptides of the medicinal leech <i>Hirudo medicinalis</i> on the functional activity of neutrophil granule proteins

Grigorieva

Gorudko

Grafskaia

et al. 2021

Medical academic journal

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BACKGROUND: Resistance of microorganisms caused dangerous to human health infections to traditional antibiotics is a serious problem for healthcare. In this regard, the development of new effective antimicrobial drugs and therapeutic approaches is an urgent task. Antimicrobial peptides (AMPs) are considered a promising alternative to traditional antibiotic in the fight against resistant microorganisms. AIM: The aim of this work is to study the effect of new synthesized AMPs of the medicinal leech Hirudo medicinalis (including under conditions of development of oxidative/halogenative stress) on the functional activity of neutrophils granular proteins the main effector cells of the immune system. MATERIALS AND METHODS: Myeloperoxidase peroxidase activity was assessed by the rate of o-dianisidine oxidation. Neutrophil elastase activity was determined by the fluorescence method using a specific substrate MeOSuc-AAPV-AMC. Lactoferrin iron-binding activity was assessed spectrophotometrically by the change in absorption of protein solution after addition of Fe3+ salt. Lysozyme activity was determined by the rate of M. lysodeikticus bacterial cells lysis. RESULTS: Native AMPs 536_1 and 19347_2 inhibited and 12530 increased myeloperoxidase peroxidase activity, this tendency persisted after these AMPs modification by hypochlorous acid (HOCl). In contrast to the native AMP halogenated AMP 3967_1 acquired the ability to enhance myeloperoxidase enzymatic activity. In the presence of AMP 3967_1 neutrophil elastase amidolytic activity increased insignificantly, while AMP 19347_2 inhibited neutrophil elastase activity. After HOCl modification these AMPs retained their ability to regulate neutrophil elastase activity. Synergistic effects (~20%) against gram-positive bacteria M. lysodeikticus were revealed for combination of lysozyme with AMPs 12530 and 3967_1. Inhibition lysozyme antimicrobial activity was observed in the presence of AMPs 19347_2 and 536_1, however the severity of this effect decreased after AMPs modification by HOCl. After HOCl modification AMP 3967_1 increased, while AMP 12530 on the contrary acquired the ability to inhibit lysozyme mucolytic activity. CONCLUSIONS: The use of drugs based on studied AMPs of medicinal leech will have a beneficial effect on the bodys fight against infectious agents due to the antimicrobial action of AMPs themselves. But in addition studied AMPs are capable to modulate the biological activity of own endogenous antimicrobial proteins and peptides: to enhance it, if it is necessary to eliminate pathogen and to inhibit if it necessary to protect against damage to the bodys own tissues.

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Lysozyme activity determination using peptidoglycan from the cell wall of Micrococcus lysodeikticus

Cited by 2 publications

References 11 publications

Influence of Type 2 Diabetes Mellitus on the Prevention and Treatment of Prosthetic Stomatitis

Influence of Type 2 Diabetes Mellitus on the Prevention and Treatment of Prosthetic Stomatitis

Influence of new antimicrobial peptides of the medicinal leech <i>Hirudo medicinalis</i> on the functional activity of neutrophil granule proteins

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