2008
DOI: 10.1016/j.fsi.2008.04.001
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Lysozyme gene expression and hemocyte behaviour in the Mediterranean mussel, Mytilus galloprovincialis, after injection of various bacteria or temperature stresses

Abstract: The aim of the present study was to evaluate the expression of the Mytilus galloprovincialis lysozyme gene in different in vivo stress situations, including injection of bacteria Vibrio splendidus LGP32, Vibrio anguillarum or Micrococcus lysodeikticus, as well as heat shock at 30 degrees C and cold stress at 5 degrees C. Injection of V. splendidus LGP32 resulted in: (i) a general down-regulation of lysozyme gene expression, as quantified by Q-PCR; (ii) reduction in the number of circulating hemocytes; (iii) de… Show more

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Cited by 93 publications
(58 citation statements)
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References 49 publications
(58 reference statements)
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“…V. anguillarum is one of the most common pathogens to marine bivalves. The immune responses of marine bivalves to V. anguillarum challenges have been extensively investigated at molecular levels [7,10]. Fig.…”
Section: Effects Of M Luteus and V Anguillarum On The Proteome Of Hmentioning
confidence: 99%
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“…V. anguillarum is one of the most common pathogens to marine bivalves. The immune responses of marine bivalves to V. anguillarum challenges have been extensively investigated at molecular levels [7,10]. Fig.…”
Section: Effects Of M Luteus and V Anguillarum On The Proteome Of Hmentioning
confidence: 99%
“…In addition, as a filter-feeder, M. galloprovincialis can accumulate a large amount of bacteria from the seawater and therefore has been used in immunity studies of marine aquaculture animals [7]. Traditional approaches in the immune innate system in M. galloprovincialis consist of histological studies on the lesions and the identification and characterization of a certain class of molecules in the immune network at gene and protein levels [7][8][9][10]. For example, infection of vibrios (Vibrio splendidus and Vibrio anguillarum) could significantly up-regulated the expression of antioxidant enzymes (catalase and glutathione transferase), lysozymes and metallothioneins which are involved in immune defense systems in M. galloprovincialis [8].…”
Section: Introductionmentioning
confidence: 99%
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“…Hemocyte cDNA samples of mussels challenged with one injection of bacteria (Vibrio splendidus LGP32, Vibrio anguillarum, Micrococcus lysodeikticus) were those previously used for analysis of lysozyme gene expression (Li et al, 2008). Fungal challenge consisted of one injection of 50 ll of PBS-NaCl (2 mM KH 2 HPO 4 , 10 mM Na 2 HPO 4 , 3 mM KCl, 600 mM NaCl in distilled water, pH 7.4) containing 10 4 spores of the filamentous fungus, Fusarium oxysporum, or of the yeast, Candida albicans, collected after 3 days of cultivation at room temperature on PDA (Potato Dextrose Agar, Sigma) in Petri dishes.…”
Section: Challenges With Bacteria and Fungimentioning
confidence: 99%
“…Negative control reactions contained water in place of cDNA template and were included in each run to ensure absence of contamination. House keeping gene expression was represented by 28S ribosomal RNA, using the forward primer 5 0 -AAGCGAGAAAAGAAACTAAC-3 0 and the reverse primer 5 0 -TTTACCTCTAAGCGGTTTCAC-3 0 , as previously validated (Cellura et al, 2007;Li et al, 2008) with an annealing temperature of 65°C. Calibration curves were obtained using 10-fold serial dilutions of MIF or 28S rRNA amplicons in 10 lg/ ml sonicated salmon sperm DNA (Sigma).…”
Section: Q-pcr Using the Sybr Green Chemistrymentioning
confidence: 99%