Lysozyme uptake into pharmaceutical grade fucoidan/chitosan polyelectrolyte multilayers under physiological conditions

Benbow, Natalie L.; Sebben, Damien A.; Karpiniec, Samuel S.; Stringer, Damien N.; Krasowska, Marta; Beattie, David A.

doi:10.1016/j.jcis.2020.01.030

Cited by 16 publications

(14 citation statements)

References 74 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, it is likely that the incubation of the multilayers into the bacteria solution which is composed of cell culture media is leading to film degradation (mass loss) and possible nisin Z loss from the film. While this has not been confirmed for this system, the same phenomenon has been seen by our group for films composed of chitosan and fucoidan 52 upon their immersion in PBS solution, where pH (and ionic strength changes) caused both swelling and mass loss from the films. S4, SI.…”

Section: Resultssupporting

confidence: 59%

“…The complexation of nisin and chitosan is quite common 6,50 , suggesting that hydrogen bonding likely occurs with CS in the studied system, in addition to the electrostatic interactions between nisin Z and CAR. Given the FTIR data, we hypothesise that the nisin Z, adsorbing onto an oppositely charged polymer and being a relatively small molecule (3.3 kDa), diffuses within the film upon adsorption 51 , a phenomenon that we have observed recently for lysozyme into a similar PEM composed of CS and fucoidan 52 .…”

Section: Resultsmentioning

confidence: 73%

“…Thickness measurements were achieved by scratching the PEM with the tip of a stainless steel needle in order to expose the silicon wafer substrate beneath (previous work by our group has shown no damage to the underlying silicon/native silica layer using this methodology 52 ). The edge of a scratch was identified with an optical microscope and scanned to reveal film thickness (i.e.…”

Section: Atomic Force Microscopymentioning

confidence: 99%

See 2 more Smart Citations

Incorporation and antimicrobial activity of nisin Z within carrageenan/chitosan multilayers

Webber

Namivandi-Zangeneh

Drozdek

et al. 2021

Sci Rep

Self Cite

View full text Add to dashboard Cite

An antimicrobial peptide, nisin Z, was embedded within polyelectrolyte multilayers (PEMs) composed of natural polysaccharides in order to explore the potential of forming a multilayer with antimicrobial properties. Using attenuated total reflection Fourier transform infrared spectroscopy (ATR FTIR), the formation of carrageenan/chitosan multilayers and the inclusion of nisin Z in two different configurations was investigated. Approximately 0.89 µg cm−2 nisin Z was contained within a 4.5 bilayer film. The antimicrobial properties of these films were also investigated. The peptide containing films were able to kill over 90% and 99% of planktonic and biofilm cells, respectively, against Staphylococcus aureus and methicillin-resistant Staphylococcus aureus (MRSA) strains compared to control films. Additionally, surface topography and wettability studies using atomic force microscopy (AFM) and the captive bubble technique revealed that surface roughness and hydrophobicity was similar for both nisin containing multilayers. This suggests that the antimicrobial efficacy of the peptide is unaffected by its location within the multilayer. Overall, these results demonstrate the potential to embed and protect natural antimicrobials within a multilayer to create functionalised coatings that may be desired by industry, such as in the food, biomaterials, and pharmaceutical industry sectors.

show abstract

Section: Resultssupporting

confidence: 59%

Section: Resultsmentioning

confidence: 73%

Section: Atomic Force Microscopymentioning

confidence: 99%

See 1 more Smart Citation

Incorporation and antimicrobial activity of nisin Z within carrageenan/chitosan multilayers

Webber

Namivandi-Zangeneh

Drozdek

et al. 2021

Sci Rep

Self Cite

View full text Add to dashboard Cite

show abstract

“…This technique probes chemical and structural changes in molecules due to adsorption or environmental changes, such as pH, ionic strength, concentration, and temperature. Numerous studies have focused on the adsorption of proteins and the study of their secondary structure, − while others dealt with polysaccharide or polyelectrolyte adsorption − on various solid substrates, including gold nanoparticles, with the aim of ATR–FTIR. To the best of our knowledge, no study focused on the adsorption of such highly complex AGP macromolecules on solid surfaces by in situ ATR–FTIR.…”

Section: Introductionmentioning

confidence: 99%

In Situ ATR Spectroscopy Study of the Interaction of Acacia senegal Gum with Gold Nanoparticles Films at the Solid–Liquid Interface

Davantès,

Nigen,

Sanchez

et al. 2023

Langmuir

View full text Add to dashboard Cite

The adsorption process of Acacia gum (A. senegal), a complex heteropolysaccharide, was followed by using a spectroscopic method to unravel the relative contribution of the protein moieties and the carbohydrate blocks on the adsorption process. In situ ATR−FTIR was used to investigate the kinetics and conformational changes associated with the adsorption of A. senegal gum on gold nanoparticle films (Au-NPs) at different pHs. The results of this thorough study highlighted the adsorption of A. senegal gum through its protein moieties, in particular, AGPs of low molecular weight and high protein content, close to the Au-NPs surface. Isotherm experiments, by gradually increasing the concentration, showed that the gum adsorption was heterogeneous and followed the Freundlich model for the amide part, while the polysaccharide part followed the Langmuir model. In addition, the hydration and structural organization of the gum layer depended on the gum concentration. A. senegal gum adsorbed irreversibly on Au-NPs whatever the pHs, but the adsorbed layer presented a different behavior depending on pH. A more aggregated and less hydrated structure was observed at acidic pH, while a very hydrated and continuous layer was detected at higher pH. The secondary structure analysis through amide III band revealed a change in the gum secondary structure at high pH with the increase in β-turn while random coil decreased.

show abstract

“…Lysozyme is an important antibacterial component, catalytically hydrolyzing β (1→4) glycosidic linkages at the C 4 atom within the N-acetyl-D-glucosamine units present in chitosan. This catalytic hydrolysis justifies the antibacterial nature of lysozyme, as it selectively degrades the cell walls of microorganisms without destroying other tissues [53,54]. More recently, several studies have investigated the effects of chitosan-lysozyme conjugates on bacterial strains [55,56].…”

Section: Introductionmentioning

confidence: 99%

Anti-Biofilm Coatings Based on Chitosan and Lysozyme Functionalized Magnetite Nanoparticles

et al. 2021

View full text Add to dashboard Cite

Biofilms represent a common and increasingly challenging problem in healthcare practices worldwide, producing persistent and difficult to manage infections. Researchers have started developing antibiotic-free treatment alternatives in order to decrease the risk of resistant microbial strain selection and for the efficient management of antibiotic tolerant biofilm infections. The present study reports the fabrication and characterization of magnetite-based nanostructured coatings for producing biofilm-resistant surfaces. Specifically, magnetite nanoparticles (Fe3O4) were functionalized with chitosan (CS) and were blended with lysozyme (LyZ) and were deposited using the matrix-assisted pulsed laser evaporation (MAPLE) technique. A variety of characterization techniques were employed to investigate the physicochemical properties of both nanoparticles and nanocoatings. The biological characterization of the coatings assessed through cell viability and antimicrobial tests showed biocompatibility on osteoblasts as well as antiadhesive and antibiofilm activity against both Gram-negative and Gram-positive bacterial strains and no cytotoxic effect against human-cultured diploid cells.

show abstract

Lysozyme uptake into pharmaceutical grade fucoidan/chitosan polyelectrolyte multilayers under physiological conditions

Cited by 16 publications

References 74 publications

Incorporation and antimicrobial activity of nisin Z within carrageenan/chitosan multilayers

Incorporation and antimicrobial activity of nisin Z within carrageenan/chitosan multilayers

In Situ ATR Spectroscopy Study of the Interaction of Acacia senegal Gum with Gold Nanoparticles Films at the Solid–Liquid Interface

Anti-Biofilm Coatings Based on Chitosan and Lysozyme Functionalized Magnetite Nanoparticles

Contact Info

Product

Resources

About