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Brodersen, Kai; Zimmermann, Bernhard

doi:10.1007/978-3-476-00162-7_13

Cited by 2 publications

(4 citation statements)

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“…The presence of IgE was identified in patients with atopic contact dermatitis and healthy volunteers at concentrations ranging from 1.0–75.5 ng mL −1 . Further, one study suggested that Hepatitis B virus (HBV)‐specific IgGs were present in eccrine sweat and eccrine glands of seropositive patients . This preliminary evidence suggests that IgG and IgA levels in eccrine sweat, in particular, are sufficient to measure the seroconversion of patients with infectious diseases.…”

Section: Resultsmentioning

confidence: 99%

“…Several studies have evaluated the presence of antibody isotypes and cytokines in sweat of healthy individuals. Antibody isotypes found in sweat include IgA, IgE, and IgG . Cytokines found in sweat include interleukin (IL)‐1α, IL‐1β, IL‐6, IL‐8, IL‐31, tumor necrosis factor (TNF)‐α, and transforming growth factor (TGF)‐β .…”

Section: Introductionmentioning

confidence: 99%

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Eccrine Sweat as a Biofluid for Profiling Immune Biomarkers

Katchman

Zhu

Christen

et al. 2018

Proteomics Clinical Apps

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PurposeSweat is a relatively unexplored biofluid for diagnosis and monitoring of disease states. In this study, the proteomic profiling of immune‐related biomarkers from healthy individuals are presented.Experimental DesignEccrine sweat samples are collected from 50 healthy individuals. LC‐MS/MS is performed on two pools of sweat samples from five male and female participants. Individual sweat samples are analyzed by antibody isotyping microarrays (n = 49), human cytokine arrays (n = 30), and quantitative ELISAs for interleukin‐1α (n = 16), epidermal growth factor (n = 6), and angiogenin (n = 7).ResultsIn sweat, 220 unique proteins are identified by shotgun analysis. Detectable antibody isotypes include IgA (100% positive; median 1230 ± 28 700 pg mL−1), IgD (18%; 22.0 ± 119 pg mL−1), IgG1 (96%; 1640 ± 6750 pg mL−1), IgG2 (37%; 292 ± 6810 pg mL−1), IgG3 (71%; 74.0 ± 119 pg mL−1), IgG4 (69%; 43.0 ± 42.0 pg mL−1), and IgM (41%; 69.0 ± 1630 pg mL−1). Of 42 cytokines, three are readily detected in all sweat samples (p < 0.01). The median concentration for interleukin‐1α is 352 ± 521 pg mL−1, epidermal growth factor is 86.5 ± 147 pg mL−1, and angiogenin is 38.3 ± 96.3 pg mL−1. Multiple other cytokines are detected at lower levels.Conclusions and Clinical RelevanceSweat can be used for profiling antibodies and innate immune biomarkers.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Eccrine Sweat as a Biofluid for Profiling Immune Biomarkers

Katchman

Zhu

Christen

et al. 2018

Proteomics Clinical Apps

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“…RD-histone genes have a canonical polyadenylation signal downstream of the CS. Defects in histone mRNA 3 0 -end processing usually cause the usage of this signal, resulting in upregulation in polyA + forms and concomitant downregulation in polyA À forms (Narita et al, 2007;Yang et al, 2009;Romeo et al, 2014;Brodersen et al, 2016). We thus isolated polyA + and polyA À RNAs from Cntl and ALYREF KD cells and carried out RNA-seq separately (Figs 3A and EV3A).…”

Section: Slbp Interacts With Alyref Via Its C-terminal Regionmentioning

confidence: 99%

“…In support of this view, TREX is required for efficient histone mRNA export. Post-translational modifications occur to both ALYREF and SLBP (Borchers et al, 2006;Okada et al, 2008;Hung et al, 2010;Brodersen et al, 2016). It is possible that these modifications could regulate their interaction and modulate histone mRNA export.…”

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confidence: 99%

ALYREF links 3′‐end processing to nuclear export of non‐polyadenylated mRNA s

Fan

Wang

et al. 2019

The EMBO Journal

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The RNA‐binding protein ALYREF plays key roles in nuclear export and also 3′‐end processing of polyadenylated mRNAs, but whether such regulation also extends to non‐polyadenylated RNAs is unknown. Replication‐dependent (RD)‐histone mRNAs are not polyadenylated, but instead end in a stem‐loop (SL) structure. Here, we demonstrate that ALYREF prevalently binds a region next to the SL on RD‐histone mRNAs. SL‐binding protein (SLBP) directly interacts with ALYREF and promotes its recruitment. ALYREF promotes histone pre‐mRNA 3′‐end processing by facilitating U7‐snRNP recruitment through physical interaction with the U7‐snRNP‐specific component Lsm11. Furthermore, ALYREF, together with other components of the TREX complex, enhances histone mRNA export. Moreover, we show that 3′‐end processing promotes ALYREF recruitment and histone mRNA export. Together, our results point to an important role of ALYREF in coordinating 3′‐end processing and nuclear export of non‐polyadenylated mRNAs.

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Cited by 2 publications

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Eccrine Sweat as a Biofluid for Profiling Immune Biomarkers

Eccrine Sweat as a Biofluid for Profiling Immune Biomarkers

ALYREF links 3′‐end processing to nuclear export of non‐polyadenylated mRNA s

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