Mac‐1: a macrophage differentiation antigen identified by monoclonal antibody

Springer, Timothy A.; Galfrè, G.; Secher, David S.; Milstein, C.

doi:10.1002/eji.1830090410

Cited by 1,071 publications

(414 citation statements)

References 21 publications

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“…The present study investigates the influence of AMT on PMN-like cells. It is well known that PMN express Gr-1 (Fleming et al, 1993;Hestdal et al, 1991) and CD11b (Hickstein et al, 1987;Ho and Springer, 1982;Leenen et al, 1994;Rosen and Gordon, 1987;Springer et al, 1979), but both markers are not unique to PMN and the relation of both markers to PMN-resembling cells is therefore ambiguous. Previous findings strongly suggest that the Gr-1+ neutrophils are the predominant infiltrating cells in the corneas with HSK (Stumpf et al, 2002;).…”

Section: Discussionmentioning

confidence: 99%

On the influence of neutrophils in corneas with necrotizing HSV-1 keratitis following amniotic membrane transplantation

Bauer

Wasmuth

Hermans

et al. 2007

Experimental Eye Research

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Necrotizing herpetic stromal keratitis (HSK) in mice rapidly improved after amniotic membrane transplantation (AMT). In this study we determined the fate of polymorphonuclear neutrophils (PMN) after AMT. AMT or tarsorrhaphy (T) was performed in BALB/c mice with ulcerative HSK. After 2 days, corneas were studied histologically and by transmission electron microscopy (TEM). CD11b, Gr-1, and TUNEL-positive cells were identified. Macrophages were depleted by subconjunctival injection of dichloromethylene-diphosphonate-liposomes (Cl 2 MDP-LIP) before AMT. Corneas were studied for interleukin (IL)-1α, IL-2, interferon (IFN)-γ, CXCL1, CXCL2, and tumor necrosis factor (TNF)-α production by ELISA. PMN-enriched cell preparations cocultured with amniotic membrane (AM) or with AM and such recombinant (r) cytokines as rIL-1α, rIL-2, and rTNF-α or supernatants from activated lymphocytes were investigated by flow cytometry (Annexin-V/7-AAD and TUNEL), and a dimethylthiazolyl-diphenyltetrazoliumbromide (MTT)-viability assay. Corneas in the AMT mice had less inflammation, fewer PMN-like cells and fewer CD11b+, and Gr-1+ cells (P < 0.01), but a higher ratio of apoptotic to viable PMN-resembling cells (P < 0.01) than the T mice. Phagocytic removal of apoptotic PMN-like cells by macrophages was evident in the AMT group. After Cl 2 MDP-LIP treatment, the corneas had more cell debris and apoptotic cells with PMN-like morphology. The concentrations of IL-1 α, IL-2, CXCL1, and TNF-α were reduced in corneas of the AMT group as compared to that of the T group, while the concentration of CXCL2 was increased. Apoptosis of PMN-resembling cells was detected following cocultivation with AM, even when proinflammatory cytokines were present. Resolution of corneal inflammation in mice with necrotizing HSK after AMT is associated with increased apoptosis of PMN-like cells, reduction of pro-inflammatory cytokines, an increase of CXCL2, and increased removal of apoptotic PMN-like cells by macrophages.

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Section: Discussionmentioning

confidence: 99%

On the influence of neutrophils in corneas with necrotizing HSV-1 keratitis following amniotic membrane transplantation

Bauer

Wasmuth

Hermans

et al. 2007

Experimental Eye Research

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“…CD11b is the ␣-chain of the ␣ M ␤ 2 or CD11b/CD18 integrin (also known as Mac-1 or Complement receptor 3) and is generally considered a typical myelomonocytic marker involved in intercellular adhesion and phagocytosis (18). It is downregulated when macrophages become resident in many tissues, including the lung (19).…”

Section: Figmentioning

confidence: 99%

Accurate and simple discrimination of mouse pulmonary dendritic cell and macrophage populations by flow cytometry: Methodology and new insights

2004

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BackgroundThe need to accurately discriminate dendritic cells (DCs) and macrophages (Mphs) in mouse lungs is critical given important biological differences. However, a validated flow cytometry–based method is still lacking, resulting in much confusion between both cell types.MethodsSingle‐cell suspensions freshly obtained from collagenase‐digested lung tissue were stained with a CD11c‐specific monoclonal antibody, detected using a PE‐Cy5 or APC‐conjugated secondary reagent. Cellular immunophenotype was simultaneously explored using a panel of PE‐conjugated markers. The FL1 or FITC‐detection channel was reserved for the assessment of autofluorescence.ResultsCD11c‐bright cells were heterogeneous and displayed a bimodal distribution with regard to autofluorescence (AF). CD11c+/low‐AF cells were lineage‐negative and showed features compatible with myeloid DCs. This was confirmed by morphology, potent T‐cell stimulatory function in a mixed‐leukocyte reaction, surface expression of MHCII and costimulatory molecules, and further immunophenotypical criteria, including the expression of Mac‐1 and absence of CD8α. In contrast, CD11c+/high‐AF cells displayed the features of pulmonary Mphs, including typical Mph morphology, very weak induction of T‐cell proliferation, low to absent expression of MHCII and costimulatory molecules, and very low levels of Mac‐1 as well as F4/80. We also show that only CD11c+/high‐AF cells strongly expressed the macrophage marker MOMA‐2, while interestingly Mac‐3 was expressed at high levels by CD11c+/high‐AF and low‐AF alike.ConclusionsThis study shows that the combination of CD11c‐expression and autofluorescence is necessary and sufficient to accurately separate DCs from macrophage subpopulations in mouse lungs. © 2004 Wiley‐Liss, Inc.

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“…The sections were pre-treated with peroxide (H 2 O 2 ) to quench endogenous peroxidase activity. MoAbs specifying the following cell surface markers were employed as primary reagents: CD11b [7] (Seralab, Crawley Down, UK), CD4 [8] and CD8 [9] (Becton Dickinson, Moutain View, CA), CD5 [10], CD25 [11] and CD45R (B220) (all from Pharmingen, San Diego, CA), and biotinylated mouse MoAbs to non-polymorphic murine MHC class II (clone 13/4) [12]. Rat MoAb to murine IL-2 (clone S4B6) [13] and interferon-gamma (IFN-) (clone XMG1.2) [14], gifts from Dr T. Mossman (Calgary, Canada), were used to detect the corresponding cytokine-containing cells.…”

Section: Immunohistochemical Techniquesmentioning

confidence: 99%

Experimental T cell-mediated inflammatory reactions in the murine oral mucosa. II. Immunohistochemical characterization of resident and infiltrating cells

Ahlfors

Jönsson²,

Czerkinsky

1996

Clinical and Experimental Immunology

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SUMMARYThe nature and phenotype of infiltrating cells in DTH-like reactions elicited in the murine oral mucosa have been examined by routine histological and immunohistochemical procedures. During the first few hours that followed buccal challenge with the contact sensitizer oxazolone, a discrete lymphocytic reaction was disclosed in the oral mucosa of animals previously sensitized at skin sites, but was absent in animals that had been sensitized at buccal sites. The early lymphocytic reaction in the oral mucosa of skin-sensitized animals preceded the emergence of CD11 + polymorphonuclear cells (PMN) which was most prominent 8 h after hapten challenge and invaded the whole thickness of the oral epithelium. The PMN rapidly disappeared by 24 h. In contrast, early PMN infiltration was virtually absent in specimens from animals similarly challenged but that had been sensitized at local buccal sites. Irrespective of site of initial sensitization, inflammatory reactions developed in the oral mucosa, being maximal by 24 h. At that stage, CD11 + macrophages were the predominant cell type. Both CD4 and CD8 T lymphocytes were scattered in both lamina propria and epithelium, and their numbers were raised throughout the time period studied (2-168 h). IL-2 receptor expression was maximal 16 h post-challenge, and was paralleled by increased DNA synthesis in CD4 + and CD8 + cells, as demonstrated by paired immunohistoautoradiography. Focal accumulations of mononuclear cells containing IL-2-producing cells were readily detected as early as 2-3 h following local challenge with hapten in animals primed at skin but not at buccal sites. Maximal IL-2 staining was detected at 24 h irrespective of initial sensitization site. Interferon-gamma-producing cells were detected at 8 h post-challenge and remained increased during the first 24 h. MHC class II expression was detected on few oral mucosa cells during the first 4 h following hapten challenge, being mainly confined to dendritic-like cells. Consistent with increased numbers of macrophages, MHC class II expression was most intense in specimens obtained 8-24 h after hapten challenge. Thereafter, MHC class II expression was still observed in specimens obtained as late as 72 h, but was essentially associated with patches of basal keratinocytes. Taken together, these observations support the notion that the murine oral mucosa can serve as the site of expression of locally or remotely induced DTH reactions, but also indicate that the site of initial sensitization can profoundly affect the cellular composition of inflammatory reactions subsequent to local buccal challenge.

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Mac‐1: a macrophage differentiation antigen identified by monoclonal antibody

Cited by 1,071 publications

References 21 publications

On the influence of neutrophils in corneas with necrotizing HSV-1 keratitis following amniotic membrane transplantation

On the influence of neutrophils in corneas with necrotizing HSV-1 keratitis following amniotic membrane transplantation

Accurate and simple discrimination of mouse pulmonary dendritic cell and macrophage populations by flow cytometry: Methodology and new insights

Experimental T cell-mediated inflammatory reactions in the murine oral mucosa. II. Immunohistochemical characterization of resident and infiltrating cells

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