MacGreen mice: a novel tool to investigate inflammation following experimental stroke

Chen, Siyi; McGregor, Ailsa L.

doi:10.4172/1939-067x.1000143

Cited by 3 publications

(6 citation statements)

References 35 publications

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“…The c-fms gene encodes the receptor for macrophage colony-stimulating factor (CSF-1) and is expressed selectively in macrophage and trophoblast cell lineages in the spleen, lung, intestine, brain and thymus (Sasmono et al, 2003). We have shown previously that EGFP expression is upregulated in the ipsilateral hemisphere of MacGreen mice post stroke (Chen & Bennet, 2015). EGFP positive cells colabelled with the microglia/macrophage marker Iba1 and changes in the morphology of these cells from 24 h to 35 days reflect temporal changes in the function of microglia/macrophages within ischaemic regions.…”

Section: Discussionmentioning

confidence: 99%

“…Founder MacGreen mice (strain of origin C57BL/6 × CBA F1) were gifted by the Queensland Brain Institute, University of Queensland, Australia. Generation of the strain has been described elsewhere (Chen et al, 2017; Chen & Bennet, 2015; Sasmono et al, 2003). The MacGreen colony was maintained as homozygote, and all offspring were positive for the transgene.…”

Section: Methodsmentioning

confidence: 99%

“…The c‐fms gene encodes the receptor for macrophage colony‐stimulating factor (CSF‐1) and is expressed selectively in macrophage and monocyte cell lineages (Sasmono et al, 2003). MacGreen mice provide a model system for the study of macrophage development and function in the periphery (Lückoff et al, 2017; Sasmono & Williams, 2012) and offer a unique opportunity to visualise and investigate brain inflammation post stroke in conjunction with functional outcomes (Chen & Bennet, 2015).…”

Section: Methodsmentioning

confidence: 99%

“…MCAo was performed under isoflurane anaesthesia as previously described (Chen et al, 2017; Chen & Bennet, 2015). Briefly, the common carotid (CCA) was exposed through a midline cervical incision, where an 8.0 silicone‐coated monofilament was introduced and advanced 10 mm distal to the carotid bifurcation, occluding the origin of the MCA for 45 min.…”

Section: Methodsmentioning

confidence: 99%

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Delayed citalopram administration reduces brain inflammation and enhances skilled motor function after ischaemic stroke in ‘MacGreen’ mice

Chen

McGregor

2022

Eur J of Neuroscience

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Recent evidence suggests that treatment with antidepressants may promote functional recovery. However, the timeframe in which these pharmacological agents can influence stroke recovery is not well understood. This research investigated whether delayed administration of citalopram, used clinically in the management of post-stroke depression, could improve long-term functional recovery following experimental stroke. MacGreen mice carrying an enhanced green fluorescent protein reporter gene in monocyte and macrophage populations were subjected to 45 min occlusion of the middle cerebral artery. Animals were administered citalopram (10 mg/kg/day, n = 20) or saline (n = 20) starting 3 days after stroke for 28 days. Neurological deficits and skilled motor performance in the staircase task were recorded for 9 weeks post stroke. Grey and white matter structural lesions were quantified at Week 9, and enhanced green fluorescent protein immunohistochemistry was used to evaluate the effect of citalopram on inflammation. Twenty-five animals were included in the final analysis. Citalopram-treated animals (n = 13) showed a significant increase in impaired forepaw use in the staircase task compared with saline-treated animals (n = 12) 2, 3 and 7 weeks post stroke but no difference in neurological score at any time point examined. Citalopram treatment was associated with decreased monocyte/macrophage cell density and increased white matter tract integrity within the ipsilateral cortex. In conclusion, delayed administration of citalopram decreased brain inflammation and produced functional gains in our mouse model of stroke. Beneficial effects on skilled motor functions were long-lasting.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Delayed citalopram administration reduces brain inflammation and enhances skilled motor function after ischaemic stroke in ‘MacGreen’ mice

Chen

McGregor

2022

Eur J of Neuroscience

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“…The MacGreen model has been used to study macrophage function in lesion development (Sasmono et al, 2003). MacGreen mice were engineered to express enhanced GFP in macrophage and monocyte cells stimulated by Colony Stimulating Factor-1 (CSF-1) (Chen et al, 2015). This model was employed for the study of inflammatory pathways involved in lesion induction, but limited to CSF-1 expressing cell types.…”

Section: Green Fluorescencementioning

confidence: 99%

Endometriosis in the Mouse: Challenges and Progress Toward a ‘Best Fit’ Murine Model

et al. 2022

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Endometriosis is a prevalent gynecologic condition associated with pelvic pain and infertility characterized by the implantation and growth of endometrial tissue displaced into the pelvis via retrograde menstruation. The mouse is a molecularly well-annotated and cost-efficient species for modeling human disease in the therapeutic discovery pipeline. However, as a non-menstrual species with a closed tubo-ovarian junction, the mouse poses inherent challenges as a preclinical model for endometriosis research. Over the past three decades, numerous murine models of endometriosis have been described with varying degrees of fidelity in recapitulating the essential pathophysiologic features of the human disease. We conducted a search of the peer-reviewed literature to identify publications describing preclinical research using a murine model of endometriosis. Each model was reviewed according to a panel of ideal model parameters founded on the current understanding of endometriosis pathophysiology. Evaluated parameters included method of transplantation, cycle phase and type of tissue transplanted, recipient immune/ovarian status, iterative schedule of transplantation, and option for longitudinal lesion assessment. Though challenges remain, more recent models have incorporated innovative technical approaches such as in vivo fluorescence imaging and novel hormonal preparations to overcome the unique challenges posed by murine anatomy and physiology. These models offer significant advantages in lesion development and readout toward a high-fidelity mouse model for translational research in endometriosis.

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Delayed Varenicline Administration Reduces Inflammation and Improves Forelimb Use Following Experimental Stroke

Chen

McGregor

2017

Journal of Stroke and Cerebrovascular Diseases

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MacGreen mice: a novel tool to investigate inflammation following experimental stroke

Cited by 3 publications

References 35 publications

Delayed citalopram administration reduces brain inflammation and enhances skilled motor function after ischaemic stroke in ‘MacGreen’ mice

Delayed citalopram administration reduces brain inflammation and enhances skilled motor function after ischaemic stroke in ‘MacGreen’ mice

Endometriosis in the Mouse: Challenges and Progress Toward a ‘Best Fit’ Murine Model

Delayed Varenicline Administration Reduces Inflammation and Improves Forelimb Use Following Experimental Stroke

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