MACI - a new era?

Jacobi, Matthias; Villa, Vincent; Magnussen, Robert A.; Neyret, Philippe

doi:10.1186/1758-2555-3-10

Cited by 65 publications

(82 citation statements)

References 64 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…4. Микрофрактурирование Необходимо отметить, что упомянутые варианты стимуляции костного мозга позволяют получить хо-рошие результаты, но лишь на короткий период вре-мени [11,12].…”

Section: рис 3 спонгиализацияunclassified

“…Данный метод пользуется большой популярностью ввиду получения хороших кли-нических результатов [23,24,25]. Тем не менее, неко-торые авторы негативно высказываются о "мозаичной" аутохондропластике, сообщая о наличии таких недо-статков как появление болезненности в донорской зоне, отсутствие надежной интеграции пересаженного блока и невозможности покрытия больших дефектов, частич-ный лизис с трансформацией трансплантатов в фиброз-ную ткань [11,15]. Интересной представляется работа S. Ulstein [16], который, сравнивая результаты аутохон-дропластики с результатами микрофрактурирования, не увидел существенной разницы.…”

Section: рис 3 спонгиализацияunclassified

“…Хондроциты вводят шприцом под мембрану. Данный метод позволил получить хорошие результаты, однако осталась проблема неравномерного распределения хондроцитов в дефекте [11].…”

Section: рис 3 спонгиализацияunclassified

“…В дальнейшем эту методику модифи-цировали до более глубокой краевой резекции корти-кальной кости, вплоть до спонгиозной. Авторы счита-ют, что использование методики позволяет добиться ремиссии деградации хряща сроком до 5 лет у 70 % пациентов с лучшими результатами у молодых людей [11]. К преимуществам абразии относят равномер-ное образование регенерата и лучшее восстановление формы суставной поверхности по сравнению с тунне-лизацией [12].…”

Section: Introductionunclassified

See 3 more Smart Citations

Surgical methods of focal hyaline cartilage defect management in large joints (literature review)

Airapetov¹,

Воротников²,

Konovalov³

2017

View full text Add to dashboard Cite

Section: рис 3 спонгиализацияunclassified

Section: Introductionunclassified

See 2 more Smart Citations

Surgical methods of focal hyaline cartilage defect management in large joints (literature review)

Airapetov¹,

Воротников²,

Konovalov³

2017

View full text Add to dashboard Cite

“…However, the collagen scaffold used in the MACI technique does not provide the appropriate mechanical and biological signals for the formation of articular cartilage. MACI technique treatments have been shown to cause tissue hypertrophy, incomplete filling and limited integration with surrounding normal cartilage [20]. This shows that scaffolds with properties similar to native articular cartilage (high swelling, similar chemistry and compression properties, etc.)…”

Section: Surgical Treatmentsmentioning

confidence: 99%

Protein-based injectable hydrogels towards the regeneration of articular cartilage

Poveda-Reyes¹

View full text Add to dashboard Cite

A mis seres queridos, con ellos todo es posible "The future belongs to those who believe in the beauty of their dreams"Eleanor Roosevelt Acknowledgements 7 AcknowledgementsAfter reaching this point I would like to thank everybody that in one way or another has helped me through these four years of work.First of all, I gratefully acknowledge the financial support from the Spanish Ministry of Economy and Competitiveness through the DPI2010-20399-C04-03 and MAT2013-46467-C4-1-R projects, my FPI fellowship BES-2011-046144 and the EEBB-I-13-06179 and EEBB-I-14-08725 research stay grants.I would especially like to thank my thesis supervisor, Dr. Gloria Gallego Ferrer, for helping me in everything I needed through these four years, for giving me this opportunity and for all her efforts in my assistance. Also thanks to José Luis Gómez Ribelles for his valuable help and brilliant ideas.Thanks to the staff of the Microscopy Service at the Universitat Politècnica de València, for their guidance and help during the SEM sessions.I would also like to express my gratitude to my supervisors during my stays at other Research Centers. Thanks to Prof. Ulrica Edlund, who during my stay at the Fiber and Polymer Technology Department (KTH) in Stockholm, helped me with everything I could need and to all the people in the center (Laleh, Anas, Soheil, Bekir, Parmida, Robertus, Arturo, etc.) that helped me in the lab and made my stay easier. Thanks to Prof. Manuel Salmerón Sánchez at the Microenvironments for Medicine group (MiMe) (University of Glasgow) for giving me the opportunity to learn the basics of cell culture in his lab, his guidance and valuable help. I would also like to thank Dr. Vladimira Moulisova for teaching me everything I know of cell biology work and being so patient and friendly. And to all the people of the group (Marco, Sara, Eleni, Mark, Alex, Jake, Elie, Frankie, etc.), as they say "People make Glasgow" and they made me feel at home. I cannot forget all the people at the Center for Biomaterials and Tissue Engineering: to Laura Teruel, for being so patient with everyone, so helpful and keeping the lab in a perfect state, and all the lecturers for their help and advice, especially to Ana Vallés and Manuel Monleón, who introduced me to the wonderful field of Tissue Engineering.I would like to especially thank Tatiana C. Gamboa, who guided me in my first years in the lab. To Suhail, Rocío, Marta, Félix, Petra, Leonardo, Rebeca and Esther, I helped them in their first steps in the lab but I learnt a lot from them and they helped me a lot, both professional and personally. Also thanks to Alex Rodrigo who helped me a lot in my first cell cultures even if he had a million things to do.Thanks to all the people who have been in the "Sala de Reuniones" office: Manu, María, Line, Amparo G, Roberto, Guillermo, Álvaro, Laia, Rubén, etc., thanks for the break times, your laughs, your help and being the way you are.Special thanks to my friends, they gave me support through all these years, with them life is better.Finally, I w...

show abstract

Pericellular Matrix Formation and Atomic Force Microscopy of Single Primary Human Chondrocytes Cultured in Alginate Microgels

Fredrikson,

Brahmachary,

June

et al. 2023

Advanced Biology

View full text Add to dashboard Cite

One of the main components of articular cartilage is the chondrocyte's pericellular matrix (PCM), which is critical for regulating mechanotransduction, biochemical cues, and healthy cartilage development. Here, individual primary human chondrocytes (PHC) are encapsulated and cultured in 50 µm diameter alginate microgels using drop‐based microfluidics. This unique culturing method enables PCM formation and manipulation of individual cells. Over ten days, matrix formation is observed using autofluorescence imaging, and the elastic moduli of isolated cells are measured using AFM. Matrix production and elastic modulus increase are observed for the chondrons cultured in microgels. Furthermore, the elastic modulus of cells grown in microgels increases ≈ten‐fold over ten days, nearly reaching the elastic modulus of in vivo PCM. The AFM data is further analyzed using a Gaussian mixture model and shows that the population of PHCs grown in microgels exhibit two distinct populations with elastic moduli averaging 9.0 and 38.0 kPa. Overall, this work shows that microgels provide an excellent culture platform for the growth and isolation of PHCs, enabling PCM formation that is mechanically similar to native PCM. The microgel culture platform presented here has the potential to revolutionize cartilage regeneration procedures through the inclusion of in vitro developed PCM.

show abstract

MACI - a new era?

Cited by 65 publications

References 64 publications

Surgical methods of focal hyaline cartilage defect management in large joints (literature review)

Surgical methods of focal hyaline cartilage defect management in large joints (literature review)

Protein-based injectable hydrogels towards the regeneration of articular cartilage

Pericellular Matrix Formation and Atomic Force Microscopy of Single Primary Human Chondrocytes Cultured in Alginate Microgels

Contact Info

Product

Resources

About