Macro- and microstructural descriptions of the zebrafish (Danio rerio) liver

Oliveira, Letícia Batelli de; Leonardo, André Santos; Lima, Eduardo Maurício Mendes de

doi:10.5603/fm.a2016.0009

Cited by 7 publications

(5 citation statements)

References 10 publications

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“…This structure, comprising two layers of hepatocytes encircling sinusoidal blood vessels, is a defining characteristic of zebrafish liver histology. The present study found variations in cytoplasmic vacuolation of hepatocytes across different groups, which supports the recognized standards of zebrafish liver histology 33 . Interestingly, mild liver steatosis, as evidenced by the accumulation of fat in hepatocytes, was identified in groups subjected to high-fat diets, with noticeable variation depending on the duration of supplementation.…”

Section: Resultssupporting

confidence: 88%

Innovative high fat diet establishes a novel zebrafish model for the study of visceral obesity

Smolińska,

Sobczyński,

Szopa

et al. 2024

Sci Rep

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Obesity is a complex chronic condition associated with multiple health risks, including visceral obesity, which is particularly detrimental. To gain insight into the mechanisms underlying obesity and its associated pathologies, a novel zebrafish model was established using an innovative high-fat diet (HFD). The primary goal was to induce visceral obesity in zebrafish and study the associated structural changes. To achieve this, a unique HFD consisting of 40% beef fat (HFD40) was developed and supplemented with magnesium aluminometasilicate to improve stability in a high humidity environment. Feeding regimens were initiated for both juvenile (starting at 2 weeks post-fertilization, lasting 18 weeks) and adult zebrafish (3 months post-fertilization, 8 weeks feeding duration). The innovative dietary approach successfully induced visceral obesity in both juvenile and adult zebrafish. This new model provides a valuable tool to study obesity-related pathologies, metabolic syndrome, and potential therapeutic interventions. Most importantly, the low-cost and easy-to-prepare composition of HFD40 was seamlessly incorporated into the water without the need for separation, was readily absorbed by the fish and induced rapid weight gain in the zebrafish population. In conclusion, this study presents a novel HFD40 composition enriched with a high beef fat concentration (40%), which represents a significant advance in the development of an experimental zebrafish model for the study of visceral obesity and associated metabolic changes.

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Section: Resultssupporting

confidence: 88%

Innovative high fat diet establishes a novel zebrafish model for the study of visceral obesity

Smolińska,

Sobczyński,

Szopa

et al. 2024

Sci Rep

View full text Add to dashboard Cite

show abstract

“…One-year-old adult fish were chosen for two purposes: to retrieve enough plasma for biochemical analyses and because they can be employed to mimic human diet-induced dyslipidemia. A high vacuolization tendency has been observed in the liver of 16 to 22-month-old zebrafish fed 5% dietary lipid ( 99 ). Furthermore, lipid and glycogen vacuolization has also been observed in previous studies wherein zebrafish were fed 11 to 13% lipids ( 100 , 101 ).…”

Section: Discussionmentioning

confidence: 99%

Management of Hypercholesterolemia Through Dietary ß-glucans–Insights From a Zebrafish Model

et al. 2022

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Consumption of lipid-rich foods can increase the blood cholesterol content. β-glucans have hypocholesterolemic effect. However, subtle changes in their molecular branching can influence bioactivity. Therefore, a comparative investigation of the cholesterol-lowering potential of two β-glucans with different branching patterns and a cholesterol-lowering drug, namely simvastatin was undertaken employing the zebrafish (Danio rerio) model of diet-induced hypercholesterolemia. Fish were allocated to 5 dietary treatments; a control group, a high cholesterol group, two β-glucan groups, and a simvastatin group. We investigated plasma total cholesterol, LDL and HDL cholesterol levels, histological changes in the tissues, and explored intestinal transcriptomic changes induced by the experimental diets. Dietary cholesterol likely caused the suppression of endogenous cholesterol biosynthesis, induced dysfunction of endoplasmic reticulum and mitochondria, and altered the histomorphology of the intestine. The two β-glucans and simvastatin significantly abated the rise in plasma cholesterol levels and restored the expression of specific genes to alleviate the endoplasmic reticulum-related effects induced by the dietary cholesterol. Furthermore, the distinct patterns of transcriptomic changes in the intestine elicited by the oat and microalga β-glucans impacted processes such as fatty acid metabolism, protein catabolic processes, and nuclear division. Oat and microalgal β-glucans also altered the pattern of lipid deposition in the liver. Our study provides insights into the effectiveness of different β-glucans to alleviate dysfunctions in lipid metabolism caused by dietary cholesterol.

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“…Blood and blood cells are frequently seen in hepatic blood vessels. The muralium duplex suggested a possible mechanism for facilitating the nutrition of hepatocytes and releasing their products conveniently into the bloodstream (de Oliveira et al, 2016). The white vacuoles observed in HE stain indicate the existence of glycogen in hepatocytes.…”

Section: Livermentioning

confidence: 99%

Assessment of reproductive and developmental effects of graphene oxide on Japanese medaka (Oryzias latipes)

2020

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Macro- and microstructural descriptions of the zebrafish (Danio rerio) liver

Abstract: (Folia Morphol 2016; 75, 3: 382-387)

Cited by 7 publications

References 10 publications

Innovative high fat diet establishes a novel zebrafish model for the study of visceral obesity

Innovative high fat diet establishes a novel zebrafish model for the study of visceral obesity

Management of Hypercholesterolemia Through Dietary ß-glucans–Insights From a Zebrafish Model

Assessment of reproductive and developmental effects of graphene oxide on Japanese medaka (Oryzias latipes)

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