Macro- and microtranscriptomic evidence of the monocyte recruitment to regenerating liver after partial hepatectomy in mouse model

MARCO+ Macrophage Dynamics in Regenerating Liver after 70% Liver Resection in Mice

“…The lack of pronounced inflammatory cell infiltration in remnant livers has been described in several studies [ 27 , 28 ]. However, with the advance of methods for the observation and detection of infiltrating immune cells, eventually evidence accumulated confirming the migration of certain cells to the liver after partial hepatectomy, notably eosinophils [ 29 ], macrophages and monocytes [ 12 , 13 ], and also neutrophils [ 30 ]. Thus, despite the less conspicuous character of infiltration, the model of liver regeneration after resections in laboratory animals is generally consistent with other cases of tissue repair in vertebrates in terms of definite involvement of the immune system.…”

“…The rates of cell death for Kupffer cells after partial hepatectomy in rodents remain unspecified. Our own data indicate an increase in cell death rates of F4/80 + liver macrophages at 24 h after 70% liver resection in mice [ 13 ]. Specific contributions of the resident Kupffer cells and the arriving monocytic macrophages to this dynamic remain implicit, due to the lack of reliable immunochemical targets to make a distinction between the two subtypes.…”

“…Partial hepatectomies (resections of 70% liver volume) were carried out by the conventional Higgins and Anderson procedure [ 19 ], with two groups of comparison, of 25 intact and 27 sham-operated animals, respectively. The operated animals, housed as previously described [ 13 ], were sacrificed in a CO 2 -chamber at 0 h, 24 h, 72 h, or 7 days, at least five mice of each group for a timepoint. Cell culture experiments were carried out on pools of sorted cells collected from five animals.…”

“…Total proteins isolated from cells or tissues were separated by electrophoresis and transferred to blotting membranes as described elsewhere [ 5 , 13 ]. The membranes were preblocked, incubated overnight with antibodies to MARCO, TIM4, CD68, or GAPDH (anti-MARCO—ab 256822, 1:200; anti-TIM4—ab47637, anti-CD68-ab ab125212, anti-GAPDH—sc-25778, Abcam, Cambridge, UK), washed, and incubated with HRP-conjugated antibodies (Bio-Rad, Hercules, CA, USA).…”

“…Our own experiments demonstrated pronounced immigration of Ly6C + monocytes to the liver after 70% hepatectomy in mice. The immigration was accompanied by proliferation and a modest increase in cell death rates of macrophages within the liver, although ascribing these dynamics to a particular subtype of liver macrophages (migratory or resident) was problematic [ 13 ].…”